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Comparative proteomic analysis of olfactory rosettes in anadromous Coilia nasus and resident Coilia nasus

  • Li Zheng
  • Wen-Qiao TangEmail author
  • Ya Zhang
  • Hongyi Guo
Article
  • 5 Downloads

Abstract

The Japanese grenadier anchovy ( Coilia nasus ) undergoes upstream migration to spawning annually but can also be observed in freshwater resident populations. It has been hypothesized that anadromous adult C. nasus may utilize olfactory cues to locate spawning grounds. We firstly performed a comparative proteome analysis about olfactory rosettes in two populations to hunt for the proteomic changes. Among 5 408 identified proteins, 1 515 proteins (629 up-regulated and 886 down-regulated) were differentially expressed. Especially, several proteins and pathways associated with olfactory signaling were found to be significantly differential. Compared with resident C. nasus, the expressions of Golf protein and the sodium/calcium exchanger were significantly up-regulated in anadromous C. nasus. The expression of adenylate cyclase and regulator of G-protein signaling (RGS) were decreased. Our findings suggest a decrease in the expression of cGMP-dependent protein kinase (PKG) in anadromous C. nasus compared to resident C. nasus. The expression of Calmodulin (CaM) was increased and CaM-dependent protein kinase II (CaMKII) was decreased. In addition, KEGG pathway enrichment analysis of up-regulated proteins indicated statistically significant difference not only in olfactory transduction but also in the cGMP-PKG signal pathway. Furtherly, we sought out some proteins expressed in the same trend occurring in DEGs (differentially expressed genes) and DEPs (differentially expressed proteins) by doing the integrative analysis of proteome and transcriptome in olfactory rosettes of C. nasus.

Keyword

Coilia nasus olfaction spawning migration proteome 

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Notes

Acknowledgement

We thank Mr. ZHAO Zhengguan and Dr. SONG Xiaojing for help for sample collection, and Dr. ZHU Guoli for completing transcriptome analysis of Japanese grenadier anchovy.

Supplementary material

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References

  1. Bandoh H, Kida I, Ueda H. 2011. Olfactory responses to natal stream water in sockeye salmon by BOLD fMRI. PLoS One, 6(1): e16051.Google Scholar
  2. Barbin G P, Parker S J, McCleave J D. 1998. Olfactory clues play a critical role in the estuarine migration of silverphase American eels. Environmental Biology of Fishes, 53(3): 283–291.Google Scholar
  3. Breer H, Boekhoff I, Tareilus E. 1990. Rapid kinetics of second messenger formation in olfactory transduction. Nature, 345(6270): 65–68.Google Scholar
  4. Dittman A H, Quinn T P, Nevitt G A, Hacker B, Storm D R. 1997. Sensitization of olfactory guanylyl cyclase to a specific imprinted odorant in coho salmon. Neuron, 19(2): 381–389.Google Scholar
  5. Døving K B, Westerberg H, Johnsen P B. 1985. Role of olfaction in the behavioral and neuronal responses of Atlantic salmon, Salmo salar, to hydrographic stratification. Canadian Journal of Fisheries and Aquatic Sciences, 42(10): 1 658–1 667.Google Scholar
  6. Hirotsu T, Saeki S, Yamamoto M, Iino Y. 2000. The Ras–MAPK pathway is important for olfaction in Caenorhabditis elegans. Nature, 404(6775): 289–293.Google Scholar
  7. Hu G J, Koh J, Yoo M J, Grupp K, Chen S X, Wendel J F. 2013. Proteomic profiling of developing cotton fibers from wild and domesticated Gossypium barbadense. New Phytologist, 200(2): 570–582.Google Scholar
  8. Jiang T, Yang J, Liu H B, Shen X Q. 2012. Life history of Coilia nasus from the Yellow Sea inferred from otolith Sr:Ca ratios. Environmental Biology of Fishes, 95(4): 503–508.Google Scholar
  9. Johnstone K A, Lubieniecki K P, Koop B F, Davidson W S. 2011. Expression of olfactory receptors in different life stages and life histories of wild Atlantic salmon( Salmo salar ). Molecular Ecology, 20(19): 4 059–4 069.Google Scholar
  10. Kaupp U B. 2010. Olfactory signalling in vertebrates and insects: differences and commonalities. Nature Reviews Neuroscience, 11(3): 188–200.Google Scholar
  11. Mashukova A, Spehr M, Hatt H, Neuhaus E M. 2006. β–arrestin2–mediated internalization of mammalian odorant receptors. The Journal of Neuroscience, 26(39): 9 902–9 912.Google Scholar
  12. Mombaerts P. 1999. Molecular biology of odorant receptors in vertebrates. Annual Review of Neuroscience, 22: 487–509.Google Scholar
  13. Moon C, Jaberi P, Otto–Bruc A, Baehr W, Palczewski K, Ronnett G V. 1998. Calcium–sensitive particulate guanylyl cyclase as a modulator of cAMP in olfactory receptor neurons. The Journal of Neuroscience, 18(9): 3 195–3 205.Google Scholar
  14. Muers M. 2011. Gene expression: transcriptome to proteome and back to genome. Nature Reviews Genetics, 12(8): 518.Google Scholar
  15. Nakamura T, Gold G H. 1987. A cyclic nucleotide–gated conductance in olfactory receptor cilia. Nature, 325(6103): 442–444.Google Scholar
  16. Nickell W T, Kleene N K, Kleene S J. 2007. Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium. The Journal of Physiology, 583: 1 005–1 020.Google Scholar
  17. Noé J, Tareilus E, Boekhoff I, Breer H. 1997. Sodium/calcium exchanger in rat olfactory neurons. Neurochemistry International, 30(6): 523–531.Google Scholar
  18. Pace U, Hanski E, Salomon Y, Lancet D. 1985. Odorantsensitive adenylate cyclase may mediate olfactory reception. Nature, 316(6025): 255–258.Google Scholar
  19. Peppel K, Boekhoff I, McDonald P, Breer H, Caron M G, Lefkowitz R J. 1997. G protein–coupled receptor kinase 3(GRK3) gene disruption leads to loss of odorant receptor desensitization. The Journal of Biological Chemistry, 272(41): 25 425–25 428.Google Scholar
  20. Reisert J, Lai J, Yau K W, Bradley J. 2005. Mechanism of the excitatory Cl–response in mouse olfactory receptor neurons. Neuron, 45(4): 553–561.Google Scholar
  21. Reisert J, Matthews H R. 1998. Na +–dependent Ca 2+ extrusion governs response recovery in frog olfactory receptor cells. The Journal of General Physiology, 112(5): 529–535.Google Scholar
  22. Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. 2011. Global quantification of mammalian gene expression control. Nature, 473(7347): 337–342.Google Scholar
  23. Sinnarajah S, Dessauer C W, Srikumar D, Chen J, Yuen J, Yilma S, Dennis J C, Morrison E E, Vodyanoy V, Kehrl J H. 2001. RGS2 regulates signal transduction in olfactory neurons by attenuating activation of adenylyl cyclase III. Nature, 409(6823): 1 051–1 055.Google Scholar
  24. Vogel C, Marcotte E M. 2012. Insights into the regulation of protein abundance from proteomic and transcriptomic analyses. Nature Reviews Genetics, 13(4): 227–232.Google Scholar
  25. Watt W C, Storm D R. 2001. Odorants stimulate the ERK/mitogen–activated protein kinase pathway and activate cAMP–response element–mediated transcription in olfactory sensory neurons. The Journal of Biological Chemistry, 276(3): 2 047–2 052.Google Scholar
  26. Yamamoto Y, Hino H, Ueda H. 2010. Olfactory imprinting of amino acids in lacustrine sockeye salmon. PLoS One, 5(1): e8633, https://doi.org/10.1371/journal.pone.0008633.Google Scholar
  27. Yano K, Nakamura A. 1992. Observations on the effect of visual and olfactory ablation on the swimming behavior of migrating adult chum salmon, Oncorhynchus keta. Japanese Journal of Ichthyology, 39(1): 67–83.Google Scholar
  28. Zhu G L, Tang W Q, Wang L J, Wang C, Wang X M. 2016. Identification of a uniquely expanded V1R(ORA) gene family in the Japanese grenadier anchovy(C oilia nasus ). Marine Biology, 163: 126.Google Scholar
  29. Zhu G L, Wang L J, Tang W Q, Liu D, Yang J Q. 2014. De novo transcriptomes of olfactory epithelium reveal the genes and pathways for spawning migration in Japanese grenadier anchovy( Coilia nasus ). PLoS One, 9(8): e103832.Google Scholar

Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Li Zheng
    • 1
    • 2
  • Wen-Qiao Tang
    • 1
    • 2
    • 3
    Email author
  • Ya Zhang
    • 1
  • Hongyi Guo
    • 1
  1. 1.Shanghai Universities Key Laboratory of Marine Animal Taxonomy and EvolutionShanghaiChina
  2. 2.National Demonstration Center for Experimental Fisheries Science EducationShanghaiChina
  3. 3.Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of AgricultureShanghai Ocean UniversityShanghaiChina

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