Comparative proteomic analysis of olfactory rosettes in anadromous Coilia nasus and resident Coilia nasus

  • Li Zheng
  • Wen-Qiao TangEmail author
  • Ya Zhang
  • Hongyi Guo


The Japanese grenadier anchovy ( Coilia nasus ) undergoes upstream migration to spawning annually but can also be observed in freshwater resident populations. It has been hypothesized that anadromous adult C. nasus may utilize olfactory cues to locate spawning grounds. We firstly performed a comparative proteome analysis about olfactory rosettes in two populations to hunt for the proteomic changes. Among 5 408 identified proteins, 1 515 proteins (629 up-regulated and 886 down-regulated) were differentially expressed. Especially, several proteins and pathways associated with olfactory signaling were found to be significantly differential. Compared with resident C. nasus, the expressions of Golf protein and the sodium/calcium exchanger were significantly up-regulated in anadromous C. nasus. The expression of adenylate cyclase and regulator of G-protein signaling (RGS) were decreased. Our findings suggest a decrease in the expression of cGMP-dependent protein kinase (PKG) in anadromous C. nasus compared to resident C. nasus. The expression of Calmodulin (CaM) was increased and CaM-dependent protein kinase II (CaMKII) was decreased. In addition, KEGG pathway enrichment analysis of up-regulated proteins indicated statistically significant difference not only in olfactory transduction but also in the cGMP-PKG signal pathway. Furtherly, we sought out some proteins expressed in the same trend occurring in DEGs (differentially expressed genes) and DEPs (differentially expressed proteins) by doing the integrative analysis of proteome and transcriptome in olfactory rosettes of C. nasus.


Coilia nasus olfaction spawning migration proteome 


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We thank Mr. ZHAO Zhengguan and Dr. SONG Xiaojing for help for sample collection, and Dr. ZHU Guoli for completing transcriptome analysis of Japanese grenadier anchovy.

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Li Zheng
    • 1
    • 2
  • Wen-Qiao Tang
    • 1
    • 2
    • 3
    Email author
  • Ya Zhang
    • 1
  • Hongyi Guo
    • 1
  1. 1.Shanghai Universities Key Laboratory of Marine Animal Taxonomy and EvolutionShanghaiChina
  2. 2.National Demonstration Center for Experimental Fisheries Science EducationShanghaiChina
  3. 3.Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of AgricultureShanghai Ocean UniversityShanghaiChina

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