Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system

  • Dandan Hou
  • Weiting YuEmail author
  • Demeng Zhang
  • Lili Zhao
  • Xiudong LiuEmail author
  • Xiaojun Ma


Immobilization biocatalysis is a potential technology to improve the activity and stability of biocatalysts in nonaqueous systems for efficient industrial production. Alginate-chitosan (AC) microcapsules were prepared as immobilization carriers by emulsification-internal gelation and complexation reaction, and their contribution on facilitating the growth and metabolism of yeast cells were testified successfully in culture medium-solvent biphasic systems. The cell growth in AC microcapsules is superior to that in alginate beads, and the cells in both immobilization carriers maintain much higher activity than free cells, which demonstrates AC microcapsules can confer yeast cells the ability to resist the adverse effect of solvent. Moreover, the performance of AC microcapsules in biphasic systems could be improved by adjusting the formation of outer polyelectrolyte complex (PEC) membrane to promote the cell growth and metabolic ability under the balance of resisting solvent toxicity and permitting substrate diff usion. Therefore, these findings are quite valuable for applying AC microcapsules as novel immobilization carriers to realize the biotransformation of value-added products in aqueous-solvent biphasic systems.


alginate-chitosan (AC) microcapsules immobilization biocatalysis aqueous-solvent biphasic system cell growth 


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We thank Dr. Xin Xiong (Natural and Medical Sciences Institute at University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany), and Prof. Dr. Rumen Krastev (University Reutlingen, Alteburgstr. 150, 72762 Reutlingen, Germany) for the constructive discussion and communication about biocatalysis-related issues.


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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Environment and Chemical EngineeringDalian UniversityDalianChina
  2. 2.Affiliated Zhongshan Hospital of Dalian UniversityDalianChina
  3. 3.State Key Laboratory of Bioactive Seaweed SubstancesQingdao Brightmoon Seaweed Group Co. Ltd.QingdaoChina
  4. 4.Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina

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