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Journal of Oceanology and Limnology

, Volume 37, Issue 2, pp 767–776 | Cite as

Physiological responses in vitamin C system during hibernation in juvenile Chinese soft-shelled turtle Pelodiscus sinensis

  • Bojian Chen
  • Cuijuan NiuEmail author
  • Lin Yuan
  • Wenyi Zhang
Aquaculture and Fisheries

Abstract

Vitamin C (Vc) is an important antioxidant that helps turtles tolerating stressful environment. This work quantified changes in tissue Vc levels during winter hibernation in the soft-shelled turtle Pelodiscus sinensis, to reveal the stress response pattern of tissue Vc during hibernation and contribute basic data for turtle culture. We sampled juvenile soft-shelled turtles at pre-hibernation (17.0°C mud temperature; MT), during hibernation (5.8°C MT) and after arousal (20.1°C MT) in the field. The transcript levels of the gene encoding L-gulonolactone oxidase (GLO), the key enzyme for Vc synthesis, decreased significantly during hibernation. However, GLO activity did not match the GLO transcription patterns and remained stable during hibernation, and showed temperature-dependent kinetic characteristics. Vitamin C levels in the brain, liver, kidney, and spleen (but not muscle) all decreased significantly during hibernation, but recovered to pre-hibernation levels or even higher levels after arousal. The soft-shelled turtle endured 5 months of hibernation with no significant oxidative damage in most tissues, except in the spleen. Splenic Vc was nearly exhausted during hibernation, accompanied by a significantly elevated malonaldehyde (MDA) level. Although the high level of oxidative damage quickly decreased after arousal, the potential tissue damage in the spleen during hibernation might account for the weakened immune capacity of turtles after hibernation.

Keyword

Chinese soft-shelled turtle hibernation reactive oxygen species (ROS) L-gulonolactone oxidase oxidative stress 

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Notes

Acknowledgement

This study was approved by the Ethic and Animal Welfare Committee (EAWC) of Beijing Normal University. We are very grateful to Mr. CAO Zhendong, Dr. LIU Kai and Dr. ZHANG Zuobing for their help (technical assistance) in this study.

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Bojian Chen
    • 1
    • 2
  • Cuijuan Niu
    • 2
    Email author
  • Lin Yuan
    • 2
  • Wenyi Zhang
    • 2
  1. 1.College of Animal Science and TechnologyNorthwest Agriculture and Forestry UniversityYanglingChina
  2. 2.Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life SciencesBeijing Normal UniversityBeijingChina

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