Journal of Oceanology and Limnology

, Volume 36, Issue 6, pp 2322–2332 | Cite as

Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803

  • Jun Chen (陈军)
  • Wenjun Shi (史文军)
  • Wenjun Li (李文军)
  • Gao Chen (陈高)Email author
  • Song Qin (秦松)Email author


Synechocystis sp. PCC 6803 is a model organism widely used in cyanobacterium biology and biotechnology. To know the genetic background of substrains of Synechocystis sp. PCC 6803 is important for further research and application. In this study, we reported the genome sequences of two non-motile wild-type substrains of Synechocystis sp. PCC 6803 using whole genome resequencing. 55/56 putative single nucleotide polymorphisms (SNPs) and 8/9 Indels (insertion and deletion) were identified. Among these, 47 SNPs were found in both the GT-AR and GT-CH strains, and 8 were unique to GT-AR and 9 were unique to GT-CH. All of these variations were annotated in metabolism pathway referred to KEGG database. Meanwhile, the deletion in slr0332 gene was detected in these two strains, which attributed to the non-motile phenotype of them and suggested that the insertion in spkA gene was not essential for non-motile phenotype. These resequencing data provide the genetic background information of these two strains and highlighted the microevolution over decades of laboratory cultivation.


Synechocystis sp. PCC 6803 genome resequencing non-motile genetic background 


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Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803


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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jun Chen (陈军)
    • 1
    • 2
  • Wenjun Shi (史文军)
    • 1
    • 2
  • Wenjun Li (李文军)
    • 1
  • Gao Chen (陈高)
    • 3
    Email author
  • Song Qin (秦松)
    • 1
    Email author
  1. 1.Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantaiChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Biotechnology Research CentreShandong Academy of Agricultural SciencesJinanChina

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