Journal of Oceanology and Limnology

, Volume 36, Issue 6, pp 2358–2367 | Cite as

Effects of feeding time on complement component C7 expression in Pelteobagrus vachellii subject to bacterial challenge

  • Ting Shao (邵婷)
  • Chuanjie Qin (覃川杰)Email author
  • Huiguo Duan (段辉国)
  • Dengyue Yuan (袁登越)
  • Zhengyong Wen (文正勇)
  • Jun Wang (王钧)
  • Fanglan Ge (葛芳兰)


Shifting the feeding time for fish from daytime to nighttime could alter their digestive behavior, disturb their metabolism, and may affect immune-related genes. This study aimed to clone complement component C7 and analyze the different expression of C7 mRNA in fish fed during either the day or at night and then challenged with Aeromonas hydrophila infection. The Pv-C7 cDNA of Pelteobagrus vachellii contained 2 647 bp with an open reading frame encoding a protein of 818 amino acids. Multiple sequences analysis indicated that Pv-C7 included eight domains, which was similar to results for other species. Quantitative PCR analysis showed that Pv-C7 was mainly expressed in the liver, spleen, intestine and head kidney tissues of healthy P. vachellii. Quantitative PCR analysis showed that C7 mRNA transcript in the liver, spleen and head kidney also increased significantly when the fish were fed at nighttime (20:00). In addition, the expression of Pv-C7 mRNA significantly increased with A. hydrophila challenge in the liver (48–96 h), spleen and head kidney (12–96 h) tissues of P. vachellii. Pv-C7 mRNA expression of the fish fed at nighttime showed significant higher than that in the fish fed at day time at 12–48 h in head kidney and 12–24 h in spleen. This study indicates that altering the feeding time from daytime to nighttime could increase Pv-C7 mRNA expression, and feeding time may affect the immune response involving C7.


Pelteobagrus vachellii complement component C7 shifting feeding time clock 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arjona A, Sarkar D K. 2006. Evidence supporting a circadian controlof natural killer cell function. Brain, Behavior, and Immunity, 20 (5): 469–476.CrossRefGoogle Scholar
  2. Bossi F, Rizzi L, Bulla R, Debeus A, Tripodo C, Picotti P, Betto E, Macor P, Pucillo C, Würzner R, Tedesco F. 2009. C7 is expressed on endothelial cells as a trap for the assembling terminal complement complex and may exert antiinflammatory function. Blood, 113 (15): 3 640–3 648.CrossRefGoogle Scholar
  3. Bozek K, Relógio A, Kielbasa S M, Heine M, Dame C, Kramer A, Herzel H. 2009. Regulation of clock–controlled genes in mammals. PLoS One, 4 (3): e4882.CrossRefGoogle Scholar
  4. Castanon–Cervantes O, Wu M W, Ehlen J C, Paul K, Gamble K L, Johnson R L, Besing R C, Menaker M, Gewirtz A T, Davidson A J. 2010. Dysregulation of inflammatory responses by chronic circadian disruption. The Journal of Immunology, 185 (10): 5 796–5 805.CrossRefGoogle Scholar
  5. del Pozo A, Montoya A, Vera L M, Sánchez–Vázquez F J. 2012. Daily rhythms of clock gene expression, glycaemia and digestive physiology in diurnal/nocturnal European seabass. Physiology & Behavior, 106 (4): 446–450.CrossRefGoogle Scholar
  6. Fisheries and Fisheries Administration Bureau of the Ministry of Agriculture. 2017. China Fishery Statistical Yearbook. China Agriculture Press, Beijing. p.25.Google Scholar
  7. Fujito N T, Sugimoto S, Nonaka M. 2010. Evolution of thioester–containing proteins revealed by cloning and characterization of their genes from a cnidarian sea anemone, Haliplanella lineate. Developmental & Comparative Immunology, 34 (7): 775–784.CrossRefGoogle Scholar
  8. Gao B, Radaeva S, Park O. 2009. Liver natural killer and natural killer T cells: immunobiology and emerging roles in liver diseases. J ournal of Leukoc yte Biology, 86 (3): 513–528.CrossRefGoogle Scholar
  9. González S, Martínez–Borra J, López–Larrea C. 2003. Cloning and characterization of human complement component C7 promoter. Genes and Immunity, 4 (1): 54–59.CrossRefGoogle Scholar
  10. Guo B Y, Wu C W, Lv Z M, Liu C L. 2016. Characterisation and expression analysis of two terminal complement components: C7 and C9 from large yellow croaker, Larimichthys crocea. Fish & Shell fish Immunology, 51: 211–219.CrossRefGoogle Scholar
  11. Kalsbeek A, Scheer F A, Perreau–Lenz S, La Fleur S E, Yi C X, Fliers E, Buijs R M. 2011. Circadian disruption and SCN controlof energy metabolism. FEBS Letters, 585 (10): 1 412–1 426.CrossRefGoogle Scholar
  12. Katagiri T, Hirono I, Aoki T. 1999. Molecular analysis of complement component C8β and C9 cDNAs of Japanese flounder, Paralichthys olivaceus. Immunogenetics, 50 (1–2): 43–48.CrossRefGoogle Scholar
  13. Knutsson A. 2003. Health disorders of shift workers. Occupational Medicine, 53 (2): 103–108.CrossRefGoogle Scholar
  14. Lazado C C, Skov P V, Pedersen P B. 2016. Innate immune defenses exhibit circadian rhythmicity and differential temporal sensitivity to a bacterial endotoxin in Nile tilapia ( Oreochromis niloticus ). Fish & Shellfish Immunology, 55: 613–622.CrossRefGoogle Scholar
  15. Li L, Chang M X, Nie P. 2007. Molecular cloning, promoter analysis and induced expression of the complement component C9 gene in the grass carp Ctenopharyngodon idella. Veterinary Immunology and Immunopathology, 118 (3–4): 270–282.CrossRefGoogle Scholar
  16. Li M F. 2010. Progress on study on biology of Pelteeobaagrus fulvidraco (Richardson). Modern Fisheries Information, 25 (9): 16–22. (in Chinese with English abstract)Google Scholar
  17. Liu J G, Malkani G, Shi X Y, Meyer M, Cunningham–Runddles S, Ma X J, Sun Z S. 2006. The circadian clock Period 2 gene regulates gamma interferon production of NK cells in host response to lipopolysaccharide–induced endotoxic shock. Infection and Immunity, 74 (8): 4 750–4 756.CrossRefGoogle Scholar
  18. Liu W, Jiang L H, Dong X L, Liu X X, Kang L S, Wu C W. 2016. Molecular characterization and expression analysis of the large yellow croaker ( Larimichthys crocea ) complement component C6 after bacteria challenge. Aquaculture, 458: 107–112.CrossRefGoogle Scholar
  19. Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using Q real–time quantitative PCR and the 2–ΔΔC T method. Methods, 25 (4): 402–408.CrossRefGoogle Scholar
  20. Logan R W, Sarkar D K. 2012. Circadian nature of immune function. Molecular and Cellular Endocrinology, 349 (1): 82–90.CrossRefGoogle Scholar
  21. Montoya A, López–Olmeda J F, Garayzar A B S, Sánchez–Vázquez F J. 2010a. Synchronization of daily rhythms of locomotor activity and plasma glucose, cortisoland thyroid hormones to feeding in Gilthead seabream ( Sparus aurata ) under a light–dark cycle. Physiology & Behavior, 101 (1): 101–107.CrossRefGoogle Scholar
  22. Montoya A, López–Olmeda J F, Yúfera M, Sánchez–Muros M J, Sánchez–Vázquez F J. 2010b. Feeding time synchronises daily rhythms of behaviour and digestive physiology in gilthead seabream ( Sparus aurata ). Aquaculture, 306 (1–4): 315–321.CrossRefGoogle Scholar
  23. Morgan B P, Marchbank K J, Longhi M P, Harris C L, Gallimore A M. 2005. Complement: central to innate immunity and bridging to adaptive responses. Immunology Letters, 97 (2): 171–179.CrossRefGoogle Scholar
  24. Mukherji A, Kobiitaa A, Chambona P. 2015. Shifting the feeding of mice to the rest phase creates metabolic alterations, which, on their own, shift the peripheral circadian clocks by 12 hours. Proceedings of the National Academy of Sciences of the Unite States of America, 112 (48): e6 683–E6 690.CrossRefGoogle Scholar
  25. Muller–Eberhard H J. 1986. The membrane attack complex of complement. Annual Review of Immunology, 4 (1): 503–528.CrossRefGoogle Scholar
  26. Niroshana Wickramaarachchi W D, Whang I, Kim E, Lim B S, Jeong H–B, De Zoysa M, Oh M–J, Jung S–J, Yeo S–Y, Yeon K S, Park H–C, Lee J. 2013. Genomic characterization and transcriptional evidence for the involvement of complement component 7 in immune response of rock bream ( Oplegnathus fasciatus ). Developmental & Comparative Immunology, 41 (1): 44–49.CrossRefGoogle Scholar
  27. Oishi K, Ohkura N, Kadota K, Kasamatsu M, Shibusawa K, Matsuda J, Machida K, Horie S, Ishida N. 2006. Clock mutation affects circadian regulation of circulating blood cells. Journal of Circadian Rhythms, 4: 13.CrossRefGoogle Scholar
  28. Phillips D J, Savenkova M I, Karatsoreos I N. 2015. Environmental disruption of the circadian clock leads to altered sleep and immune responses in mouse. Brain, Behavior, and Immunity, 47: 14–23.CrossRefGoogle Scholar
  29. Qin C J, Gong Q, Wen Z Y, Yuan D Y, Shao T, Wang J, Li H T. 2017. Transcriptome analysis of the spleen of the darkbarbel catfish Pelteobagrus vachellii in response to Aeromonas hydrophila infection. Fish & Shell fish Immunology, 70: 498–506.CrossRefGoogle Scholar
  30. Qin C J, Shao T. 2015. The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli. Chinese Journal of Oceanology and Limnology, 33 (3): 597–603.CrossRefGoogle Scholar
  31. Scheidereit C. 2006. IκB kinase complexes: gateways to NF–κB activation and transcription. Oncogene, 25 (51): 6 685–6 705.CrossRefGoogle Scholar
  32. Shen Y B, Zhang J B, Xu X Y, Fu J J, Li J L. 2012. Expression of complement component C7 and involvement in innate immune responses to bacteria in grass carp. Fish & Shell fish Immunology, 33 (2): 448–454.CrossRefGoogle Scholar
  33. Sun Y Y, Wang R X, Xu T J. 2013. Conserved structural complement component C3 in miiuy croaker Miichthys miiuy and their involvement in pathogenic bacteria induced immunity. Fish & Shellfish Immunology, 35 (1): 184–187.CrossRefGoogle Scholar
  34. Wang S C, Gao Y H, Shu C, Xu T J. 2015. Characterization and evolutionary analysis of duplicated C7 in miiuy croaker. Fish & Shellfish Immunology, 45 (2): 672–679.CrossRefGoogle Scholar
  35. Wang S C, Wang R X, Xu T J. 2013. The evolutionary analysis on complement genes reveals that fishes C3 and C9 experience different evolutionary patterns. Fish & Shellfish Immunology, 35 (6): 2 040–2 045.CrossRefGoogle Scholar
  36. Witzel–Schlomp K, Rittner C, Schneider P M. 2001. The human complement C9 gene: structural analysis of the 5’ gene region and genetic polymorphism studies. European Journal of Immunogenetics, 28 (5): 515–522.CrossRefGoogle Scholar
  37. Yang R B, Xie C X, Wei K J, Zheng W Y, Lei C S, Feng K. 2006. The daily feeding rhythms of juvenile yellow catfish, Pelteobagrus fulvidraco at different feeding frequencies. Journal of Huazhong Agricultural University, 25 (3): 274–276. (in Chinese with English abstract)Google Scholar
  38. Zheng K K, Zhu X M, Han D, Yang Y X, Lei W, Xie S Q. 2010. Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae. Aquaculture, 299 (1–4): 121–127.CrossRefGoogle Scholar

Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ting Shao (邵婷)
    • 1
    • 2
  • Chuanjie Qin (覃川杰)
    • 2
    Email author
  • Huiguo Duan (段辉国)
    • 2
  • Dengyue Yuan (袁登越)
    • 2
  • Zhengyong Wen (文正勇)
    • 2
  • Jun Wang (王钧)
    • 2
  • Fanglan Ge (葛芳兰)
    • 1
  1. 1.College of Life ScienceSichuan Normal UniversityChengduChina
  2. 2.College of Life Science, Neijiang Normal UniversityKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze RiverNeijiangChina

Personalised recommendations