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Journal of Oceanology and Limnology

, Volume 36, Issue 6, pp 2288–2296 | Cite as

Immunohistochemical identification of dynorphin A and Kappa opioid receptor-1 in the digestive system of scallop Chlamys farreri

  • Hushan Sun (孙虎山)Email author
  • Yiyan Wang (王宜艳)
  • Xiaoli Liu (刘小莉)
  • Dongwu Liu (刘东武)
Article
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Abstract

Little is known about the roles of dynorphin and Kappa opioid receptor (KOR) in mollusks. In this study, we aim to determine the distribution of dynorphin A and KOR-1 in the digestive system of the scallop Chlamys farreri. Using immunohistochemical staining, we confirmed the expression of dynorphin A and KOR-1 in the digestive system of C. farreri. Dynorphin A immunopositive cells were identified in intestine and hepatopancreas. In intestine, small and spherical dynorphin A immunopositive cells (4–9 μm in diameter) were scattered among the long columnar epithelial cells (ECs). In hepatopancreas, cells containing masses (5–14 μm in diameter) of dynorphin A immunopositive products were observed in epithelium of acinis. These immunopositive cells may be synthetic and/or secretory cells of dynorphin A. Dynorphin A immunoreactive products were commonly observed in epithelium and connective tissue (CT) of labial palps, mouth labia and stomach, which presented in forms of grains, fibers or flakes. KOR-1 immunoreactive material was observed in ECs and CTs of labial palps, mouth labia and stomach, intestine and hepatopancreas. The distribution of both dynorphin A and KOR-1 in the digestive organs suggests an involvement of dynorphin via KOR-1 in the functional regulation of the digestive system of C. farreri.

Keyword

Chlamys farreri dynorphin digestive system kappa opioid receptors immunohistochemistry 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hushan Sun (孙虎山)
    • 1
    Email author
  • Yiyan Wang (王宜艳)
    • 1
  • Xiaoli Liu (刘小莉)
    • 1
  • Dongwu Liu (刘东武)
    • 2
  1. 1.School of Life ScienceLudong UniversityYantaiChina
  2. 2.Analysis and Testing CenterShandong University of TechnologyZiboChina

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