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Journal of Oceanology and Limnology

, Volume 36, Issue 6, pp 2351–2357 | Cite as

Cryopreservation of strip spawned sperm using programmable freezing technique in the blue mussel Mytilus galloprovincialis

  • Yibing Liu (刘一兵)
  • Shiwen Liu (刘诗文)
  • Bingli Liu (刘冰莉)
  • Jianguang Qin (秦建光)
  • Tong Xu (许通)
  • Xiaoxu Li (李孝绪)Email author
Article
  • 16 Downloads

Abstract

In this study, a programmable freezing technique has been developed for strip spawned sperm in the blue mussel, Mytilus galloprovincialis. The optimized key parameters include cooling rate, endpoint temperature, thawing temperature, sugar addition and sperm to oocyte ratio. The sperm quality was assessed by the fertilization rate or the integrity of sperm component and organelle. The highest post-thaw sperm fertilization rate was 91%, which was produced with sperm cryopreserved in 8% dimethyl sulfoxide at the cooling rate of -4°C/min from 2°C to -30°C before being plunged into liquid nitrogen for at least 12 h, thawed in a 20°C seawater bath and fertilized at sperm to egg ratio of 50 000:1. The addition of glucose, sucrose or trehalose to 8% dimethyl sulfoxide could not further improve fertilization rates. The fluorescent assessments showed that the post-thaw sperm plasma membrane integrity and acrosome integrity were significantly damaged in comparison with fresh sperm.

Keyword

blue mussel Mytilus galloprovincialis strip spawning sperm cryopreservation 

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Notes

Acknowledgement

We thank Mr Andy Dyle for the provision of blue mussel broodstock and Mr Mark Gluis of SARDI for technical support.

References

  1. Adams S L, Smith J F, Roberts R D, Janke A R, Kaspar H F, Tervit H R, Pugh P A, Webb S C, King N G. 2004. Cryopreservation of sperm of the Pacific oyster ( Crassostrea gigas ): development of a practical method for commercial spat production. Aquaculture, 242 (1–4): 271–282.CrossRefGoogle Scholar
  2. Anchordoguy T, Crowe J H, Griffin F J, Clark W H. 1988. Cryopreservation of sperm from the marine shrimp sicyonia ingentis. Cryobiology, 25 (3): 238–243.CrossRefGoogle Scholar
  3. Cabrita E, Sarasquete C, Martínez–Páramo S, Robles V, Beirão J, Pérez–Cerezales S, Herráez M P. 2010. Cryopreservation of fish sperm: applications and perspectives. Journal of Applied Ichthyology, 26 (5): 623–635.CrossRefGoogle Scholar
  4. Clulow J R, Mansfield L J, Morris L H A, Evans G, Maxwell W M C. 2008. A comparison between freezing methods for the cryopreservation of stallion spermatozoa. Animal Reproduction Science, 108 (3–4): 298–308.CrossRefGoogle Scholar
  5. Di Matteo O, Langellotti A L, Masullo P, Sansone G. 2009. Cryopreservation of the Mediterranean mussel ( Mytilus galloprovincialis ) spermatozoa. Cryobiology, 58 (2): 145–150.CrossRefGoogle Scholar
  6. Dong Q X, Eudeline B, Huang C J, Allen S K, Tiersch T R. 2005. Commercial–scale sperm cryopreservation of diploid and tetraploid pacific oysters, Crassostrea gigas. Cryobiology, 50 (1): 1–16.CrossRefGoogle Scholar
  7. Dupré E, Guerrero A. 2011. Cryopreservation of Macha surf clam spermatozoa. in: Tiersch T R, Green C C eds. Cryopreservation in Aquatic Species. 2 nd edn. World Aquaculture Society, Baton Rouge. p.574–580.Google Scholar
  8. Gao D Y, Critser J K. 2000. Mechanisms of cryoinjury in living cells. ILAR Journal, 41 (4): 187–196.CrossRefGoogle Scholar
  9. Gómez–Fernández J, Gómez–Izquierdo E, Tomás C, Mocé E, De Mercado E. 2012. Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation. Animal Reproduction Science, 133 (1–2): 109–116.CrossRefGoogle Scholar
  10. Gwo J C, Chen C W, Cheng H Y. 2002. Semen cryopreservation of small abalone ( Haliotis diversicolor supertexa ). Theriogenology, 58 (8): 1 563–1 578.CrossRefGoogle Scholar
  11. Gwo J C. 2008. Cryopreservation of small abalone ( Haliotis diversicolor supertexa ) semen. in: Cabrita E, Robles V, Herráez P eds. Methods in Reproductive Aquaculture: Marine and freshwater Species. CRC Press, New York. 480p.Google Scholar
  12. Hassan M M, Qin J G, Li X X. 2015. Sperm cryopreservation in oysters: a review of its current status and potentials for future application in aquaculture. Aquaculture, 438: 24–32.CrossRefGoogle Scholar
  13. Herráez P, Cabrita E, Robles V. 2012. Fish gamete and embryo cryopreservation: state of the art. in: Fletcher G L, Rise M L eds. Aquaculture Biotechnology. Wiley–Blackwell, Oxford, UK. p.303–317.Google Scholar
  14. Hopkins B K, Herr C. 2010. Factors affecting the successful cryopreservation of honey bee ( Api s mellifera ) spermatozoa. Apidologie, 41 (5): 548–556.CrossRefGoogle Scholar
  15. Ieropoli S, Masullo P, Santo M D E, Sansone G. 2004. Effects of extender composition, cooling rate and freezing on the fertilisation viability of spermatozoa of the Pacific oyster ( Crassostrea gigas ). Cryobiology, 49 (3): 250–257.CrossRefGoogle Scholar
  16. Jafaroghli M, Khalili B, Farshad A, Zamiri M J. 2011. The effect of supplementation of cryopreservation diluents with sugars on the post–thawing fertility of ram semen. Small Ruminant Research, 96 (1): 58–63.CrossRefGoogle Scholar
  17. Kang K H, Kim J M, Kim Y H. 2004. Short–term storage and cryopreservation of abalone ( Haliotis discus hannai ) sperm. The Korean Journal of Malacology, 20 (1): 17–26.Google Scholar
  18. Kawamoto T, Narita T, Isowa K, Aoki H, Hayashi M, Komaru A, Ohta H. 2007. Effects of cryopreservation methods on post–thaw motility of spermatozoa from the Japanese pearl oyster, Pinctada fucata martensii. Cryobiology, 54 (1): 19–26.CrossRefGoogle Scholar
  19. Lazo C S, Pita I M. 2012. Effect of temperature on survival, growth and development of Mytilus galloprovincialis larvae. Aquaculture Research, 43 (8): 1 127–1 133.CrossRefGoogle Scholar
  20. Li C, Li J, Xue Q Z. 2000. Cryopreservation of the spermatozoa of Chlamys ( Azumapecten ) farreri. Marine Fisheries Research, 21 (1): 57–62. (in Chinese with English abstract)Google Scholar
  21. Liu B L, Liu Y B, Liu S W, Xu T, Liu Q, Li X X. 2016a. Cryopreservation of strip spawned sperm using nonprogrammable freezing technique in the blue mussel Mytilus galloprovincialis. Aquaculture Research, 47 (12): 3 888–3 898.CrossRefGoogle Scholar
  22. Liu Y B, Li X X, Robinson N, Qin J G. 2015a. Sperm cryopreservation in marine mollusk: a review. Aquaculture International, 23 (6): 1 505–1 524.CrossRefGoogle Scholar
  23. Liu Y B, Li X X, Xu T, Robinson N, Qin J G. 2014a. Improvement in non–programmable sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata. Aquaculture, 434: 362–366.CrossRefGoogle Scholar
  24. Liu Y B, Li X X, Xu T, Robinson N, Qin J G. 2016b. Greenlip abalone ( Haliotis laevigata Donovan, 1808) sperm cryopreservation using a programmable freezing technique and testing the addition of amino acid and vitamin. Aquaculture Research, 47 (5): 1 499–1 510.CrossRefGoogle Scholar
  25. Liu Y B, Xu T, Robinson N, Qin J G, Li X X. 2014b. Cryopreservation of sperm in farmed Australian greenlip abalone Haliotis laevigata. Cryobiology, 68 (2): 185–193.CrossRefGoogle Scholar
  26. Liu Y B, Xu T, Robinson N, Qin J G, Li X X. 2015b. Cryopreservation of sperm in farmed blacklip abalone ( Haliotis rubra Leach, 1814). Aquaculture Research, 46 (11): 2 628–2 636.CrossRefGoogle Scholar
  27. Lyons L, Jerry D R, Southgate P C. 2005. Cryopreservation of black–lip pearl oyster ( Pinctada margaritifera, L.) spermatozoa: effects of cryoprotectants on spermatozoa motility. Journal of Shellfish Research, 24 (4): 1 187–1 190.CrossRefGoogle Scholar
  28. Paniagua–Chavez C G, Buchanan J T, Tiersch T R. 1998. Effect of extender solutions and dilution on motility and fertilizing ability of eastern oyster sperm. Journal of Shellfish Research, 17: 231–237.Google Scholar
  29. Pettersen A K, Turchini G M, Jahangard S, Ingram B A, Sherman C D H. 2010. Effects of different dietary microalgae on survival, growth, settlement and fatty acid composition of blue mussel ( Mytilus galloprovincialis ) larvae. Aquaculture, 309 (1–4): 115–124.CrossRefGoogle Scholar
  30. Purdy P H. 2006. A review on goat sperm cryopreservation. Small Ruminant Research, 63 (3): 215–225.CrossRefGoogle Scholar
  31. Rota A, Rota A, Martini M, Milani C, Romagnoli S. 2005. Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing. Reproduction Nutrition Development, 45 (1): 29–37.CrossRefGoogle Scholar
  32. Salinas–Flores L, Paniagua–Chavez C G, Jenkins J A, Tiersch T R. 2005. Cryopreservation of sperm of red abalone ( Haliotis rufescens ). Journal of Shellfish Research, 24 (2): 415–420.CrossRefGoogle Scholar
  33. Stanic P, Tandara M, Sonicki Z, Simunic V, Radakovic B, Suchanek E. 2000. Comparison of protective media and freezing techniques for cryopreservation of human semen. European Journal of Obstetrics & Gynecology and Reproductive Biology, 91 (1): 65–70.CrossRefGoogle Scholar
  34. Suquet M, Dreanno C, Fauvel C, Cosson J, Billard R. 2000. Cryopreservation of sperm in marine fish. Aquaculture Research, 31 (3): 231–243.CrossRefGoogle Scholar
  35. Tiersch T R. 2008. Strategies for commercialization of cryopreserved fish semen. Revista Brasileira de Zootecnia, 37 suplemento especial: 15–19.Google Scholar
  36. Vitiello V, Carlino P A, Del Prete F, Langellotti A L, Sansone G. 2011. Effects of cooling and freezing on the motility of Ostrea edulis (L., 1758) spermatozoa after thawing. Cryobiology, 63 (2): 118–124.CrossRefGoogle Scholar
  37. Viveiros A T M, Lock E J, Woelders H, Komen J. 2001. Influence of cooling rates and plunging temperatures in an interrupted slow–freezing procedure for semen of the African catfish, Clarias gariepinus. Cryobiology, 43 (3): 276–287.CrossRefGoogle Scholar
  38. Zhang X M, Li X X, Clarke S, Li X. 2012. The development of Pacific oysters Crassostrea gigas produced using cryopreserved sperm. In: Qin J G ed. Oysters: Physiology, Ecological Distribution and Mortality. Nova Science, New York. p.1–18.Google Scholar

Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yibing Liu (刘一兵)
    • 1
    • 2
  • Shiwen Liu (刘诗文)
    • 3
  • Bingli Liu (刘冰莉)
    • 3
  • Jianguang Qin (秦建光)
    • 2
  • Tong Xu (许通)
    • 3
  • Xiaoxu Li (李孝绪)
    • 4
    Email author
  1. 1.Liaoning Ocean and Fisheries Science Research InstituteDalianChina
  2. 2.College of Science and EngineeringThe Flinders University of South AustraliaAdelaideAustralia
  3. 3.College of Fisheries and Life ScienceDalian Ocean UniversityDalianChina
  4. 4.South Australian Research and Development InstituteWest BeachSouth AustraliaAustralia

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