Journal of Oceanology and Limnology

, Volume 36, Issue 6, pp 2351–2357 | Cite as

Cryopreservation of strip spawned sperm using programmable freezing technique in the blue mussel Mytilus galloprovincialis

  • Yibing Liu (刘一兵)
  • Shiwen Liu (刘诗文)
  • Bingli Liu (刘冰莉)
  • Jianguang Qin (秦建光)
  • Tong Xu (许通)
  • Xiaoxu Li (李孝绪)Email author


In this study, a programmable freezing technique has been developed for strip spawned sperm in the blue mussel, Mytilus galloprovincialis. The optimized key parameters include cooling rate, endpoint temperature, thawing temperature, sugar addition and sperm to oocyte ratio. The sperm quality was assessed by the fertilization rate or the integrity of sperm component and organelle. The highest post-thaw sperm fertilization rate was 91%, which was produced with sperm cryopreserved in 8% dimethyl sulfoxide at the cooling rate of -4°C/min from 2°C to -30°C before being plunged into liquid nitrogen for at least 12 h, thawed in a 20°C seawater bath and fertilized at sperm to egg ratio of 50 000:1. The addition of glucose, sucrose or trehalose to 8% dimethyl sulfoxide could not further improve fertilization rates. The fluorescent assessments showed that the post-thaw sperm plasma membrane integrity and acrosome integrity were significantly damaged in comparison with fresh sperm.


blue mussel Mytilus galloprovincialis strip spawning sperm cryopreservation 


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We thank Mr Andy Dyle for the provision of blue mussel broodstock and Mr Mark Gluis of SARDI for technical support.


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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yibing Liu (刘一兵)
    • 1
    • 2
  • Shiwen Liu (刘诗文)
    • 3
  • Bingli Liu (刘冰莉)
    • 3
  • Jianguang Qin (秦建光)
    • 2
  • Tong Xu (许通)
    • 3
  • Xiaoxu Li (李孝绪)
    • 4
    Email author
  1. 1.Liaoning Ocean and Fisheries Science Research InstituteDalianChina
  2. 2.College of Science and EngineeringThe Flinders University of South AustraliaAdelaideAustralia
  3. 3.College of Fisheries and Life ScienceDalian Ocean UniversityDalianChina
  4. 4.South Australian Research and Development InstituteWest BeachSouth AustraliaAustralia

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