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Journal of Oceanology and Limnology

, Volume 36, Issue 3, pp 882–891 | Cite as

Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity

  • Yuhao Sun (孙雨豪)
  • Xiaolin Chen (陈晓琳)
  • Song Liu (刘松)
  • Huahua Yu (于华华)
  • Rongfeng Li (李荣锋)
  • Xueqin Wang (王雪芹)
  • Yukun Qin (秦玉坤)
  • Pengcheng Li (李鹏程)
Article

Abstract

Heparin has been used as an anticoagulant drug for many years, but it has significant side effects. In the search for good substitutes, low molecular weight (MW) polysaccharides from Sargassum fusiforme have been examined and confirmed to possess biological activities. Here, S. fusiforme polysaccharides (SFP) were extracted and subjected to a hydrogen peroxide (H2O2) oxidation method for the preparation of low-MW SFP (LSFP). The effects of temperature, pH, and H2O2 concentration on the degradation process were also examined. Several LSFP of 36, 9, 5.7, and 2.7 kDa were obtained under different conditions, and their anticoagulant activities studied in vitro. The results showed that SFP and LSFP prolonged activated partial thromboplastin (APTT), prothrombin (PT) and thrombin times (TT) significantly, indicating that these low MW polysaccharides possessed anticoagulant activity in the intrinsic, extrinsic, and common coagulation pathways. As these effects were related to the MW of the polysaccharides in APTT and TT but not in PT, the contents of the monosaccharide fucose and sulfate and the polysaccharide MW could have exerted combined effects. The details of this mechanism require further verification.

Keyword

anticoagulant activity Sargassum fusiforme low molecular weight degradation 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yuhao Sun (孙雨豪)
    • 1
    • 2
  • Xiaolin Chen (陈晓琳)
    • 1
  • Song Liu (刘松)
    • 1
  • Huahua Yu (于华华)
    • 1
  • Rongfeng Li (李荣锋)
    • 1
  • Xueqin Wang (王雪芹)
    • 1
  • Yukun Qin (秦玉坤)
    • 1
  • Pengcheng Li (李鹏程)
    • 1
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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