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Applied Physics A

, 123:31 | Cite as

Viability assessment of bacteria using long-range surface plasmon waveguide biosensors

  • Paul Béland
  • Pierre BeriniEmail author
Article
Part of the following topical collections:
  1. Advanced Metamaterials and Nanophotonics

Abstract

We demonstrate that long-range surface plasmon waveguide biosensors are useful to monitor the quiver of immobilized live bacteria in buffer and in human urine. First, the biosensor captures bacteria selectively, based on gram, using antibodies against gram adsorbed on the surface of the waveguide through Protein G coupling. Then, analysis of the noise present on the optical output signal reveals quiver of bacteria immobilized on the waveguide. Live bacteria produce a noisy signature compared to baseline levels. The standard deviation over time of the optical power output from the biosensor increased by factors of 3–60 over that of the baseline level for Staphylococcus epidermidis and Escherichia coli immobilized selectively on waveguides.

Keywords

Human Urine Live Bacterium Output Optical Power Bacterium Immobilization Local Refractive Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We are grateful to the Ontario Centres of Excellence (OCE) for funding this work under project number 21107. We are grateful to Canadian Blood Services (Sandra Ramirez, sandra.ramirez@blood.ca) for donating two bacteria strains: Escherichia coli (E. coli) XL1 Blue and Staphylococcus epidermidis (S. epi) ATCC 12228. We are grateful to Oleksiy Krupin for assistance with the application of the surface chemistries.

Supplementary material

Supplementary material 1 (MP4 9863 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.School of Electrical Engineering and Computer ScienceUniversity of OttawaOttawaCanada
  2. 2.Department of PhysicsUniversity of OttawaOttawaCanada
  3. 3.Centre for Research in PhotonicsUniversity of OttawaOttawaCanada

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