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Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis

  • Thomas LacourEmail author
  • Philippe-Israël Morin
  • Théo Sciandra
  • Natalie Donaher
  • Douglas A. Campbell
  • Joannie Ferland
  • Marcel Babin
Original Paper

Abstract

During winter in the Arctic marine ecosystem, diatoms have to survive long periods of darkness caused by low sun elevations and the presence of sea ice covered by snow. To better understand how diatoms survive in the dark, we subjected cultures of the Arctic diatom Chaetoceros neogracilis to a prolonged period of darkness (1 month) and to light resupply. Chaetoceros neogracilis was not able to grow in the dark but cell biovolume remained constant after 1 month in darkness. Rapid resumption of photosynthesis and growth recovery was also found when the cells were transferred back to light at four different light levels ranging from 5 to 154 µmol photon m−2 s−1. This demonstrates the remarkable ability of this species to re-initiate growth over a wide range of irradiances even after a prolonged period in the dark with no apparent lag period or impact on survival. Such recovery was possible because C. neogracilis cells preserved their Chl a content and their light absorption capabilities. Carbon fixation capacity was down-regulated (ninefold dark decrease in \(P_{\text{m}}^{\text{C}}\)) much more than was the photochemistry in PSII (2.3-fold dark decrease in ETRm). Rubisco content, which remained unchanged after one month in the dark, was not responsible for the decrease in \(P_{\text{m}}^{\text{C}}\). The decrease in PSII activity was partially related to the induction of sustained non-photochemical quenching (NPQ) as we observed an increase in diatoxanthin content after one month in the dark.

Keywords

Arctic microalgae Polar night Diatom Darkness Photosynthesis Growth rate Temperature 

Notes

Acknowledgements

We thank a joint contribution to the research programs of UMI Takuvik, ArcticNet (Network Centres of Excellence of Canada), the Canada Excellence Research Chair in Remote Sensing of Canada’s New Arctic Frontier, and the Canada Research Chair program.

Supplementary material

300_2019_2507_MOESM1_ESM.docx (1.9 mb)
Supplementary material 1 (DOCX 1907 kb)

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Takuvik Joint International Laboratory, CNRS (France) & ULaval (Canada), Département de BiologieUniversité LavalQuébecCanada
  2. 2.IFREMER, Physiol & Biotechnol Algae LabsNantes Cedex 03France
  3. 3.Department of BiologyMount Allison UniversitySackvilleCanada

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