Effect of temperature on bacterial community in petroleum hydrocarbon-contaminated and uncontaminated Antarctic soil

  • C. W. Chong
  • S. Silvaraj
  • Y. Supramaniam
  • I. Snape
  • I. K. P. Tan
Original Paper

Abstract

It is generally accepted that bacterial diversity in a community confers resistance to environmental perturbation. Communities with high bacterial diversity are less likely to be impacted by environmental changes such as warming. As such, hydrocarbon-contaminated Antarctic soil that are typically characterised by low bacterial diversity and highly selective taxonomic composition are expected to be more sensitive to changes in temperature than uncontaminated Antarctic soil. To test this hypothesis, we evaluated the response of bacterial community structure to warming of hydrocarbon-contaminated and uncontaminated soil collected from Casey Station, Windmill Island, East Antarctica by using microcosms incubated at 5, 10 and 15 °C over a period of 12 weeks. Our results showed that shifts occurred in the bacterial community in relation to the incubation temperatures in both the hydrocarbon-contaminated and uncontaminated soil, with a stronger response observed in the contaminated soil. Taxa referred as comprising hydrocarbon-degrading genera such as Rhodococcus, was the most prevalent genus in the contaminated soil after incubation at 15 °C, accounting for approximately 32–50% of the total detected genera. However, there were no significant differences in the selected functional genes, potentially suggesting high levels of metabolic plasticity in the studied soil bacterial communities. Overall, we showed that hydrocarbon contamination in soil might lead to lower bacterial community stability against environmental perturbation such as temperature variation.

Keywords

Antarctic soil Bacterial community plasticity Functional gene abundance Soil microcosm 

Notes

Acknowledgements

The HC and UC Antarctic soils were kindly provided by the Australian Antarctic Division through Australian Antarctic Science Project #4036 (Remediation of Petroleum Contaminants in the Antarctic and subantarctic). This work was funded by UMRG (RP007-2012B) and YPASM fellowship (IMUR121/12). The authors thank University of Malaya and International Medical University for providing research facility and support.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

300_2018_2316_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 23 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Life Sciences, School of PharmacyInternational Medical UniversityKuala LumpurMalaysia
  2. 2.Centre of Translational Research, Institute for Research, Development and InnovationInternational Medical UniversityKuala LumpurMalaysia
  3. 3.National Antarctic Research Centre, Institute of Graduate StudiesUniversity of MalayaKuala LumpurMalaysia
  4. 4.Faculty of Science, Institute of Biological SciencesUniversity of MalayaKuala LumpurMalaysia
  5. 5.Australian Antarctic DivisionKingstonAustralia

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