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Polar Biology

, Volume 42, Issue 3, pp 541–553 | Cite as

A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12

  • Clemente Michael Vui Ling WongEmail author
  • Sook Yee Boo
  • Christopher Lok Yung Voo
  • Nursyafiqi Zainuddin
  • Nazalan Najimudin
Original Paper
  • 127 Downloads

Abstract

Glaciozyma antarctica PI12, a psychrophilic yeast from Antarctica, grows well at low temperatures. However, it is not clear how it responds and adapts to cold and freeze stresses. Hence, this project was set out to determine the cold-adaptation strategies and mechanisms of G. antarctica PI12 using a transcriptomic analysis approach. G. antarctica PI12 cells, grown in rich medium at 12 °C, were exposed to freeze stress at 0 and − 12 °C for 6 h and 24 h. Their transcriptomes were sequenced and analyzed. A hundred and sixty-eight genes were differentially expressed. The yeast gene expression patterns were found to be dependent on the severity of the cold with more genes being differentially expressed at − 12 °C than at 0 °C. Glaciozyma antarctica PI12 was found to share some common adaptation strategies with other yeasts, Saccharomyces cerevisiae and Mrakia spp., but at the same time, found to have some of its own unique strategies and mechanisms. Among the unique mechanisms was the production of antifreeze protein to prevent ice-crystallization inside and outside the cell. In addition, several molecular chaperones, detoxifiers of reactive oxygen species (ROS), and transcription and translation genes were constitutively expressed in G. antarctica PI12 to enable the cells to endure the fluctuating freezing temperatures. Interestingly, G. antarctica PI12 used nitrite as an alternative terminal acceptor of electrons when the oxygen level was low to minimize disruption of energy production in the cell. These mechanisms coupled with several other common mechanisms ensured that G. antarctica PI12 adapted well to the cold temperatures.

Keywords

RNA-sequencing Differentially expressed gene (DEG) RT-qPCR Cold adaptation Antarctica 

Notes

Acknowledgments

The funding supports from the Ministry of Science, Technology, and Innovation (MOSTI), Malaysia, under the Antarctica Flagship Programme (Sub-Project 1: FP1213E036) and Science Fund Project No: 08–05-MGI-GMB001 are gratefully acknowledged. We would also like to thank Miss Teoh Chui Peng for helping us to deposit the transcriptomic data to the NCBI GeneBank, and Michelle Wong for checking and editing the language in the final draft of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019
corrected publication January 2019

Authors and Affiliations

  1. 1.Biotechnology Research InstituteUniversiti Malaysia SabahKota KinabaluMalaysia
  2. 2.School of Biological ScienceUniversiti Sains MalaysiaPenangMalaysia

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