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Ectopic expression of LoSVP, a MADS-domain transcription factor from lily, leads to delayed flowering in transgenic Arabidopsis

  • Xiaoli Tang
  • Meixia Liang
  • Junjie Han
  • Jieshan Cheng
  • Hongxia Zhang
  • Xiaohua LiuEmail author
Original Article

Abstract

Key message

A MADS-domain transcription factor LoSVP , which could delay flowering through vernalization pathway, was isolated from lily.

Abstract

MADS-domain transcription factors play important roles in plant growth and development, especially in the transition from vegetative phase to reproductive phase. However, their functions in bulbous flowering plants are largely unknown. In this work, a SHORT VEGETATIVE PHASE (SVP) encoding genes LoSVP from oriental lily was isolated. Bioinformatic analyses demonstrated that LoSVP encodes a type II MADS-box protein containing a conserved MADS-box, as well as a conserved K-box domain. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed ubiquitous expression of LoSVP in various tissues, including petals, stamens, pistils, leaves and scales. Real-time polymerase chain reaction (PCR) analyses demonstrated that LoSVP was predominantly expressed in the early stage of developing flowers. Constitutive expression of LoSVP in Arabidopsis led to significantly delayed flowering of transgenic plants. These results suggest that LoSVP is involved in plant flowering and could be used as a potential candidate gene for the genetic regulation of flowering time in higher plants.

Keywords

MADS-box Lily LoSVP Transgenic plant 

Notes

Acknowledgements

We thank Ms. Jessie Zhang (Faculty of Health Sciences, McMaster University, Canada) for her critical reading and editing of this paper.

Author contribution statement

XT, ML, JH, and JC performed the experiments and analyzed the data. HZ and XL conceived the study. HZ and XL wrote the manuscript. All authors read and agreed at the last version of the manuscript.

Funding

This work has been jointly supported by the following grants: the National Mega Project of GMO Crops of China [2016ZX08004-002-006]; the Key R & D project of Shandong Province [2017NC210012, 2018GNC110007]; The Modern Agricultural Industry Technology System Innovation Team of Shandong Province of China (SDAIT-02-05); the National Natural Science Foundation of China (31870576).

Compliance with ethical standards

Conflict of interest

We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Xiaoli Tang
    • 1
    • 3
  • Meixia Liang
    • 1
    • 3
  • Junjie Han
    • 2
  • Jieshan Cheng
    • 1
    • 3
  • Hongxia Zhang
    • 1
    • 3
    • 4
  • Xiaohua Liu
    • 1
    • 3
    Email author
  1. 1.College of AgricultureLudong UniversityYantaiChina
  2. 2.Yantai Academy of Agricultural SciencesYantaiChina
  3. 3.Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of ShandongLudong UniversityYantaiChina
  4. 4.Institute for Advanced Study of Coastal EcologyLudong UniversityYantaiChina

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