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Banana MaBZR1/2 associate with MaMPK14 to modulate cell wall modifying genes during fruit ripening

  • Wei Shan
  • Yu-Fan Guo
  • Wei Wei
  • Jian-Ye Chen
  • Wang-Jin Lu
  • De-Bao Yuan
  • Xin-Guo SuEmail author
  • Jian-Fei KuangEmail author
Original Article

Abstract

Key message

Banana MaBZR1/2 interact with MaMPK14 to enhance the transcriptional inhibition of cell wall modifying genes including MaEXP2, MaPL2 and MaXET5.

Abstract

Fruit ripening and softening, the major attributes to perishability in fleshy fruits, are modulated by various plant hormones and gene expression. Banana MaBZR1/2, the central transcription factors of brassinosteroid (BR) signaling, mediate fruit ripening through regulation of ethylene biosynthesis, but their possible roles in fruit softening as well as the underlying mechanisms remain to be determined. In this work, we found that MaBZR1/2 directly bound to and repressed the promoters of several cell wall modifying genes such as MaEXP2, MaPL2 and MaXET5, whose transcripts were elevated concomitant with fruit ripening. Moreover, yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated that MaBZR1/2 physically interacted with a mitogen-activated protein kinase MaMPK14, and this interaction strengthened the MaBZR1/2-mediated transcriptional inhibitory abilities. Collectively, our study provides insight into the mechanism of MaBZR1/2 contributing to fruit ripening and softening, which may have potential for banana molecular improvement.

Keywords

Banana fruit BZR Cell wall modification Protein–protein interaction Transcriptional regulation 

Abbreviations

1-MCP

1-Methylcyclopropene

BAK1

BRI1-associated receptor kinase 1

BES1

Brassinosteroid Insensitive 1-EMS-Suppressor 1

BiFC

Bimolecular fluorescence complementation

BIN2

Brassinosteroid insensitive 2

BR

Brassinosteroid

BRI1

Brassinosteroid insensitive 1

BZR1

Brassinazole resistant 1

EMSA

Electrophoretic mobility shift assay

EXP

Expansin

MAPK

Mitogen-activated protein kinase

MAPKK

Mitogen-activated protein kinase kinase

MAPKKK

Mitogen-activated protein kinase kinase kinase

PCR

Polymerase chain reaction

PL

Pectate lyase

TF

Transcription factor

XET

Xyloglucan endotransglycosylase

Y2H

Yeast two-hybrid

Notes

Acknowledgements

This work was funded by the National Natural Science Foundation of China (Grant no. 31401922, 31772021), Guangdong Special Support Program (Grant no. 2017TQ04N512), China Agriculture Research System (Grant no. CARS-31-11), and Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams.

Author contribution statement

JK and XS conceived and designed the research. WS and YG carried out most of the experiments. WW performed some of the experiments. JC, WL, and DY analyzed the data. JK, XS, JC and WL wrote the manuscript. All the authors read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

Supplementary material

299_2019_2471_MOESM1_ESM.doc (44 kb)
Supplementary material 1 (DOC 44 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of HorticultureSouth China Agricultural UniversityGuangzhouPeople’s Republic of China
  2. 2.Guangdong Food and Drug Vocational CollegeGuangzhouPeople’s Republic of China
  3. 3.Hainan Key Laboratory of Banana Genetic Improvement, Haikou Experimental StationChinese Academy of Tropical Agricultural SciencesHaikouPeople’s Republic of China

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