Transient overexpression of E2Fb triggers cell divisions in pavement cells of Nicotiana benthamiana leaves
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Agrobacterium-mediated transient overexpression of E2Fb triggers new cell divisions in pavement cells of Nicotiana benthamiana leaves.
Transient transformation in Nicotiana benthamiana enables the study of multiple biological processes in a simple and fast manner. Here, we describe that, upon A. tumefaciens-mediated transient overexpression of the cell cycle regulator E2Fb from either Arabidopsis thaliana or N. benthamiana, cell divisions occur in epidermal pavement cells in N. benthamiana leaves, following a sequence of events that encompasses the nucleus taking a central position and being surrounded by chloroplasts, nuclear division, and formation of a new wall that divides the initial cell in two. Our results indicate that transient expression in N. benthamiana can be used to study cell division in plants, from DNA replication to cell wall formation, in a simple, controlled, and rapid manner.
KeywordsNicotiana bentamiana E2Fb Cell division Pavement cell Transient expression
The authors would like to thank Shingo Nagawa and Li Tan at the Cell Biology Core Facility at the Shanghai Center for Plant Stress Biology for useful advice and excellent technical assistance.
Author contribution statement
RL-D and TJ-G conceived the project; TJ-G and HT performed experiments and analyzed data; RL-D wrote the manuscript, with input from all authors.
Research in the RL-D’s lab is funded by the Shanghai Center for Plant Stress Biology of the Chinese Academy of Sciences and the 100 Talent program of the Chinese Academy of Sciences. TJ-G was sponsored by a CAS-TWAS President’s Fellowship for International Ph.D. students.
Compliance with ethical standards
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Supplementary material 2 (WMV 9791 kb) Supplementary video 1. A dividing epidermal pavement cell of N. benthamiana transiently co-expressing YFP-AtE2Fb and the nuclear marker AtHTR5-CFP.
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