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Transcription factor VqERF114 regulates stilbene synthesis in Chinese wild Vitis quinquangularis by interacting with VqMYB35

  • Lan Wang
  • Yuejin WangEmail author
Original Article

Abstract

Key message

VqERF114 regulates stilbene synthesis by interacting with VqMYB35.

Abstract

Resveratrol is a stilbene, an important class of secondary metabolites that accumulates in some plant species, including grapevine. In the plant, these are involved in the response to attack by plant pathogens and, as a component of the human diet, they offer a range of significant health benefits. Stilbene synthase (STS), the key enzyme responsible for resveratrol synthesis, has been characterised in a small number of plant species. However, the regulatory mechanisms for stilbene synthesis are uncertain. Here, an ERF family transcription factor from Chinese wild Vitis quinquangularis, VqERF114, was characterised as an indirect regulator of stilbene synthesis. A transient overexpression assay of VqERF114 in grapevine leaves led to increased STS expression and stilbene accumulation. However, VqERF114 did not bind to the promoters of VqSTSs but the MYB transcription factor, VqMYB35, did interact with VqERF114. This interaction was confirmed by a yeast two-hybrid assay and bimolecular fluorescence complementation. Furthermore, VqMYB35 showed activation effects on the expressions of VqSTS15, VqSTS28, VqSTS42 and VqSTS46 by binding directly to the MBS elements in their promoters. Co-overexpression of VqERF114 and VqMYB35 resulted in higher VqSTSs expression and more stilbene synthesis. These results demonstrate that VqERF114 regulates stilbene synthesis by interacting with VqMYB35.

Keywords

Chinese wild Vitis VqERF114 VqMYB35 Stilbene synthase Resveratrol 

Notes

Acknowledgements

This work was funded by the National Natural Science Foundation of China (Grant No. 31672129). The research was carried out by the Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China. The authors specifically thank Dr. Alexander (Sandy) Lang from RESCRIPT Co. (New Zealand) for useful comments and language editing, which have greatly improved the manuscript.

Author contribution statement

YW designed and initiated this study. LW carried out the experiments and analysed the results. LW wrote the manuscript and YW revised the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2019_2456_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 15 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of HorticultureNorthwest A&F UniversityYanglingChina
  2. 2.Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest ChinaMinistry of AgricultureYanglingChina
  3. 3.State Key Laboratory of Crop Stress Biology in Arid AreasNorthwest A&F UniversityYanglingChina

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