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Plant Cell Reports

, Volume 38, Issue 8, pp 951–963 | Cite as

The tomato MADS-box gene SlMBP9 negatively regulates lateral root formation and apical dominance by reducing auxin biosynthesis and transport

  • Anzhou Li
  • Guoping Chen
  • Xiaohui Yu
  • Zhiguo Zhu
  • Lincheng Zhang
  • Shengen Zhou
  • Zongli HuEmail author
Original Article

Abstract

Key message

Overexpression of SlMBP9 reduced auxin biosynthesis and transport, and negatively regulated lateral root formation and apical dominance.

Abstract

MADS-box transcription factors play a critical role in plant development. In this study, we describe SlMBP9, a novel MADS-box gene that is expressed in the roots of tomato plants. Tomato lines that over- or under-expressed SlMBP9 were generated using a transgenic approach. The number of lateral roots (LRs) were reduced in SlMBP9-overexpressing lines but slightly increased in SlMBP9-silenced lines. A physiological index revealed that the auxin content significantly decreased in the root maturation zone of the overexpression lines. In addition, gene expression analysis revealed that the expression of the polar auxin transporter genes PIN1 and ABCB19/MDR1 and genes involved in auxin biosynthesis was downregulated in the stems of overexpression lines, which is consistent with the reduced accumulation of auxin in the root maturation zone. Exogenous indole-3-acetic acid (auximone) rescued the lateral root phenotypes of the SlMBP9-overexpressing lines. Overexpression of SlMBP9 resulted in dwarf plants, enhanced lateral buds and reduced the gibberellin content in the stems. Together, these results suggest that SlMBP9 plays a negative role in the process of auxin biosynthesis and transport.

Keywords

Lateral root SlMBP9 Tomato Dwarf plant Lateral bud 

Abbreviations

IAA

Indole-3-acetic acid

LR

Lateral root

GA

Gibberellin

Notes

Acknowledgements

This work was supported by National Natural Science Foundation of China (No. 31572129), and the National Natural Science Foundation of Chongqing of China (No. cstc2015jcyjA80026).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

299_2019_2417_MOESM1_ESM.docx (596 kb)
Supplementary material 1 (DOCX 595 kb)
299_2019_2417_MOESM2_ESM.docx (25 kb)
Supplementary material 2 (DOCX 25 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Anzhou Li
    • 1
  • Guoping Chen
    • 1
  • Xiaohui Yu
    • 1
  • Zhiguo Zhu
    • 1
  • Lincheng Zhang
    • 1
  • Shengen Zhou
    • 1
  • Zongli Hu
    • 1
    Email author
  1. 1.Laboratory of Molecular Biology of Tomato, Bioengineering CollegeChongqing UniversityChongqingPeople’s Republic of China

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