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Genome-wide identification, phylogeny, and expression analysis of Sec14-like PITP gene family in sugarcane

  • Mao Hua-ying
  • Wang Wen-ju
  • Su Wei-hua
  • Su Ya-chun
  • Liu Feng
  • Li Cong-na
  • Wang Ling
  • Zhang Xu
  • Xu Li-ping
  • Que You-xiongEmail author
Original Article

Abstract

Key message

Six Sec14-like PITP genes from sugarcane were identified, two of them were cloned, and their biological functions were characterized indicating their involvement in plant defense against biotic and abiotic stresses.

Abstract

Sec14, a phosphatidylinositol transfer protein (PITP) is widely present in eukaryotes. In this study, the structure and expression patterns of six Sec14-like PITP genes (ScSEC14-1, ScSEC14p, ScSFH1, ScSFH2, ScPATL1, and ScPATL2) from sugarcane were analyzed, and two of them (ScSEC14-1 and ScSEC14p) were cloned and functionally verified. Phylogenetic analysis divided these genes into four groups, including group I (ScSFH1 and ScSFH2), group II (ScPATL1 and ScPATL2), Group III (ScSEC14p), and group V (ScSEC14-1). qRT-PCR analysis showed tissue-specific expression of these genes, primarily in the root, leaf, and bud tissues. They responded differently to SA, MeJA, and ABA stresses. ScSEC14-1, ScSEC14p, and ScSFH2 were upregulated by CuCl2 and CdCl2, while ScSEC14-1, ScSFH1, ScSFH2, and ScPATL1 were upregulated by PEG and NaCl. When infected by Sporisorium scitamineum, the transcripts of ScSFH1, ScSFH2, ScPATL1, and ScPATL2 were upregulated in the resistant genotype Yacheng 05-179, while those of ScSEC14-1 and ScSEC14p were upregulated in the susceptible genotype ROC22. Subcellular localization showed that ScSEC14-1 and ScSEC14p were mainly localized in the plasma membrane and cytoplasm. Enhanced growth of Escherichia coli BL21 cells expressing ScSEC14-1 and ScSEC14p showed high tolerance to NaCl and mannitol stresses. The transient overexpression of ScSEC14-1 and ScSEC14p in Nicotiana benthamiana leaves enhanced its resistance to the infection of tobacco pathogens Ralstonia solanacearum and Fusarium solani var. coeruleum. We can conclude the involvement of ScSEC14-1 and ScSEC14p in the defense against biotic and abiotic stresses, which should facilitate further research on Sec14-like PITP gene family, especially its regulatory mechanisms in sugarcane.

Keywords

Sugarcane Sporisorium scitamineum Phosphatidylinositol transfer protein Salt and drought stresses Transient expression 

Notes

Acknowledgements

This work was funded by the Natural Science Foundation of Fujian Province, China (2015J06006), the National Natural Science Foundation of China (31871688, 31671752, and 31101196), Microarray Analysis and Key Gene Mining of Sugarcane in response to smut pathogen (KFA17267A), the Earmarked Fund for the China Agricultural Research System (CARS-17), and the Program for New Century Excellent Talents in Fujian Province University (JA14095).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Mao Hua-ying
    • 1
  • Wang Wen-ju
    • 1
  • Su Wei-hua
    • 1
  • Su Ya-chun
    • 1
  • Liu Feng
    • 1
  • Li Cong-na
    • 1
  • Wang Ling
    • 1
  • Zhang Xu
    • 1
  • Xu Li-ping
    • 1
  • Que You-xiong
    • 1
    Email author
  1. 1.Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of AgricultureFujian Agriculture and Forestry UniversityFuzhouChina

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