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Single and multiple gene knockouts by CRISPR–Cas9 in maize

  • Nicolas M. Doll
  • Laurine M. Gilles
  • Marie-France Gérentes
  • Christelle Richard
  • Jeremy Just
  • Yannick Fierlej
  • Virginia M. G. Borrelli
  • Ghislaine Gendrot
  • Gwyneth C. Ingram
  • Peter M. Rogowsky
  • Thomas WidiezEmail author
Original Article

Abstract

Key message

The analysis of 93 mutant alleles in 18 genes demonstrated that CRISPR–Cas9 is a robust tool for targeted mutagenesis in maize, permitting efficient generation of single and multiple knockouts.

Abstract

CRISPR–Cas9 technology is a simple and efficient tool for targeted mutagenesis of the genome. It has been implemented in many plant species, including crops such as maize. Here we report single- and multiple-gene mutagenesis via stably transformed maize plants. Two different CRISPR–Cas9 vectors were used allowing the expression of multiple guide RNAs and different strategies to knockout either independent or paralogous genes. A total of 12 plasmids, representing 28 different single guide RNAs (sgRNAs), were generated to target 20 genes. For 18 of these genes, at least one mutant allele was obtained, while two genes were recalcitrant to sequence editing. 19% (16/83) of mutant plants showed biallelic mutations. Small insertions or deletions of less than ten nucleotides were most frequently observed, regardless of whether the gene was targeted by one or more sgRNAs. Deletions of defined regions located between the target sites of two guide RNAs were also reported although the exact deletion size was variable. Double and triple mutants were created in a single step, which is especially valuable for functional analysis of genes with strong genetic linkage. Off-target effects were theoretically limited due to rigorous sgRNA design and random experimental checks at three potential off-target sites did not reveal any editing. Sanger chromatograms allowed to unambiguously class the primary transformants; the majority (85%) were fully edited plants transmitting systematically all detected mutations to the next generation, generally following Mendelian segregation.

Keywords

CRISPR Gene editing Maize SDN1 Mutagenesis Zea mays 

Abbreviations

bp

Base pairs

Cas9

CRISPR-associated protein 9

CRISPR

Clustered regularly interspaced short palindromic repeats

DSB

Double-strand break

EMS

Ethyl methanesulfonate

ESR

Embryo surrounding region

HR

Homologous recombination

MMEJ

Microhomology-mediated end joining

NHEJ

Non-homologous end joining

PAM

Protospacer adjacent motif

SDN1

Site-directed nuclease 1

sgRNA

Single guide RNA

shRNA

Short hairpin RNA, also referring to scaffold RNA

T-DNA

Transfer DNA

Notes

Acknowledgements

We thank Bing Yang (Iowa State University) for sharing the Iowa vectors prior to publication, Jean-Philippe Pichon (Biogemma SA) for providing unpublished genomic sequences of genotype A188, Justin Berger, Patrice Bolland and Alexis Lacroix for maize culture, Isabelle Desbouchages and Hervé Leyral for buffers and media preparation, as well as Sandrine Chaignon, Jérôme Laplaige and Edwige Delahaye for technical assistance. We acknowledge support by the Investissement d’Avenir program of the French National Agency of Research for the project GENIUS (ANR-11-BTBR-0001_GENIUS) to PMR and by the INRA Plant Science and Breeding Division for the project SeedCom to TW. NMD is funded by a PhD fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche. LMG and YF are supported by CIFRE fellowships of the ANRT (Grants 2015/0777 and 2018/0480). VMGB is supported by the Doctoral School on the Agro-Food System (Agrisystem) of Università Cattolica del Sacro Cuore (Italy).

Compliance with ethical standards

Conflict of interest

LMG is employed by Limagrain Europe. YF is employed by MAS seed. PMR is part of the GIS-BV (“Groupement d’Intérêt Scientifique Biotechnologies Vertes”).

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratoire Reproduction et Développement des PlantesUniv Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRALyonFrance
  2. 2.Limagrain Europe SAS, Research CentreChappesFrance
  3. 3.MAS SeedsHaut-MaucoFrance
  4. 4.Department of Sustainable Crop ProductionUniversità Cattolica del Sacro CuorePiacenzaItaly

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