Plant Cell Reports

, Volume 37, Issue 11, pp 1451–1470 | Cite as

Thidiazuron-induced abnormalities in plant tissue cultures

  • Yaser Hassan DewirEmail author
  • NurmansyahEmail author
  • Yougasphree NaidooEmail author
  • Jaime A. Teixeira da SilvaEmail author


Thidiazuron (TDZ) is a proven effective and potent synthetic plant growth regulator for organogenic, regeneration, and developmental pathways, including axillary and adventitious shoot proliferation, somatic embryogenesis, and in vitro flowering. TDZ has facilitated the establishment of in vitro cultures for several plant species, especially woody and recalcitrant plants, which has enabled their genetic transformation and improvement. Despite the effectiveness and advantages of using TDZ, several drawbacks are associated with its application in plant tissue culture. This review addresses the morphological, physiological, and cytogenetic abnormalities associated with the use of TDZ in vitro, and provides a summary of these abnormalities in several plant species.


Albinism DNA polymorphism In vitro culture Micropropagation Somaclonal variations Physiological disorders 



Y.H. Dewir and Y. Naidoo extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University for funding this research work through ISPP# 0064.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Plant Production Department, College of Food and Agriculture SciencesKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Department of Horticulture, Faculty of AgricultureKafrelsheikh UniversityKafr El-SheikhEgypt
  3. 3.School of Life SciencesUniversity of KwaZulu-NatalDurbanSouth Africa
  4. 4.IkenobeJapan

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