Help or hindrance: how do microtubule-based forces contribute to genome damage and repair?

  • Cassi Estrem
  • Jeffrey K. MooreEmail author


Forces generated by molecular motors and the cytoskeleton move the nucleus and genome during many cellular processes, including cell migration and division. How these forces impact the genome, and whether cells regulate cytoskeletal forces to preserve genome integrity is unclear. We recently demonstrated that, in budding yeast, mutants that stabilize the microtubule cytoskeleton cause excessive movement of the mitotic spindle and nucleus. We found that increased nuclear movement results in DNA damage and increased time to repair the damage through homology-directed repair. Our results indicate that nuclear movement impairs DNA repair through increased tension on chromosomes and nuclear deformation. However, the previous studies have shown genome mobility, driven by cytoskeleton-based forces, aids in homology-directed DNA repair. This sets up an apparent paradox, where genome mobility may prevent or promote DNA repair. Hence, this review explores how the genome is affected by nuclear movement and how genome mobility could aid or hinder homology-directed repair.


DNA damage HDR Dynein Microtubule Cytoskeleton Nucleus 



This work was supported by National Institutes of Health Grant no. R01GM-112893 (to J.K.M.).


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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Cell and Developmental BiologyUniversity of Colorado School of MedicineAuroraUSA
  2. 2.Department of Brain and Cognitive SciencesMassachusetts Institute of TechnologyCambridgeUSA

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