Opportunities for new studies of nuclear DNA replication enzymology in budding yeast
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Three major eukaryotic DNA polymerases, Polymerases α, δ, and ε (Pols α, δ, and ε), perform the fundamental process of DNA synthesis at the replication fork both accurately and efficiently. In trying to understand the necessity and flexibility of the polymerase usage, we recently reported that budding yeast cells lacking Pol ε exonuclease and polymerase domains (pol2-16) survive, but have severe growth defects, checkpoint activation, increased level of dNTP pools as well as significant increase in the mutation rates. Herein, we suggest new opportunities to distinguish the roles of Pol ε from those of two other eukaryotic B-family DNA polymerases, Pols δ and ζ.
KeywordsGenome stability DNA replication DNA polymerases Polymerase epsilon (Pol ε) Polymerase delta (Pol δ) Polymerase zeta (Pol ζ)
We thank Mercy Arana and Jessica Williams for critical reading of and thoughtful comments on the manuscript.
Division of Intramural Research of the NIH, NIEHS [Z01 ES065070 to T.A.K.].
- Burgers PMJ, Kunkel TA (2017) Eukaryotic DNA replication fork. Annu Rev Biochem 86:417–438. https://doi.org/10.1146/annurev-biochem-061516-044709 CrossRefGoogle Scholar
- Loeb LA, Springgate CF, Battula N (1974) Errors in DNA replication as a basis of malignant changes. Cancer Res 34:2311–2321Google Scholar
- Pelosini C, Martinelli S, Ceccarini G et al (2014) Identification of a novel mutation in the polymerase delta 1 (POLD1) gene in a lipodystrophic patient affected by mandibular hypoplasia, deafness, progeroid features (MDPL) syndrome. Metabolism 63:1385–1389. https://doi.org/10.1016/j.metabol.2014.07.010 CrossRefGoogle Scholar