Not quite the SSAme: unique roles for the yeast cytosolic Hsp70s
- 284 Downloads
The Heat Shock Protein 70s (Hsp70s) are an essential family of proteins involved in folding of new proteins and triaging of damaged proteins for proteasomal-mediated degradation. They are highly conserved in all organisms, with each organism possessing multiple highly similar Hsp70 variants (isoforms). These isoforms have been previously thought to be identical in function differing only in their spatio-temporal expression pattern. The model organism Saccharomyces cerevisiae (baker’s yeast) expresses four Hsp70 isoforms Ssa1, 2, 3 and 4. Here, we review recent findings that suggest that despite their similarity, Ssa isoforms may have unique cellular functions.
KeywordsChaperone Hsp70 Ssa Isoform Evolution
This work was supported by NCI R15CA208773 (AWT).
- Boorstein WR, Craig EA (1990) Structure and regulation of the SSA4 HSP70 gene of Saccharomyces cerevisiae. J Biol Chem. 265(31):18912–18921Google Scholar
- Boorstein WR, Ziegelhoffer T, Craig EA (1994) Molecular evolution of the HSP70 multigene family. J Mol Evo 38(1):1–17Google Scholar
- Gao BC, Biosca J, Craig EA, Greene LE, Eisenberg E (1991) Uncoating of coated vesicles by yeast hsp70 proteins. J Biol Chem 266(29):19565–19571Google Scholar
- Jones GW, Masison DC (2003) Saccharomyces cerevisiae Hsp70 mutations affect [PSI+] prion propagation and cell growth differently and implicate Hsp40 and tetratricopeptide repeat cochaperones in impairment of [PSI+]. Genetics 163(2):495–506Google Scholar
- Jung G, Jones G, Wegrzyn RD, Masison DC (2000) A role for cytosolic hsp70 in yeast [PSI(+)] prion propagation and [PSI(+)] as a cellular stress. Genetics 156(2):559–570Google Scholar
- Sager RA, Woodford MR, Backe SJ, Makedon AM, Baker-Williams AJ, DiGregorio BT, et al. (2019) Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90. Cell Rep 26(5), 1344–56 e5.Google Scholar
- Truman AW, Millson SH, Nuttall JM, Mollapour M, Prodromou C, Piper PW (2007) In the yeast heat shock response, Hsf1-directed induction of Hsp90 facilitates the activation of the Slt2 (Mpk1) mitogen-activated protein kinase required for cell integrity. Eukaryot Cell 6(4):744–752CrossRefGoogle Scholar
- van Leeuwen J, Pons C, Mellor JC, Yamaguchi TN, Friesen H, Koschwanez J, et al. (2016) Exploring genetic suppression interactions on a global scale. Science 354(6312).Google Scholar