Escaping nuclear decay: the significance of mRNA export for gene expression
- 343 Downloads
In this perspective, we discuss the regulatory impact of nuclear RNA export and decay on messenger RNA (mRNA) functionality. It is well established that control of protein-coding gene expression in eukaryotes employs the regulated production of mRNA, its intra-cellular transfer to cytoplasmic ribosomes and final transcript degradation. Despite a rich body of literature on these events, an involvement of nuclear RNA decay systems remains largely unexplored. Instead, nuclear RNA degradation is often considered a quality control precaution engaged primarily in ridding cells of aberrantly processed transcripts and spurious non-coding RNA. Recent research from human and budding yeast cells, however, demonstrates that even protein-coding transcripts fall prey to nuclear decay and that this is countered by their nuclear export. Here, we outline the potential of nuclear polyA-binding proteins in tuning levels of cellular mRNA to maintain transcript homeostasis.
KeywordsNuclear RNA degradation Transcription termination Nuclear RNA export Nab2 PABPN1
- Meola N, Domanski M, Karadoulama E, Chen Y, Gentil C, Pultz D, Vitting-Seerup K, Lykke-Andersen S, Andersen JS, Sandelin A, Jensen TH (2016) Identification of a nuclear exosome decay pathway for processed transcripts. Mol Cell 64:520–533. https://doi.org/10.1016/j.molcel.2016.09.025 CrossRefGoogle Scholar
- Ogami K, Richard P, Chen Y, Hoque M, Li W, Moresco JJ, Yates JR 3rd, Tian B, Manley JL (2017) An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. Genes Dev 31(3):1257–1271. https://doi.org/10.1101/gad.302604.117 CrossRefGoogle Scholar
- Satoh R, Matsumura Y, Tanaka A, Takada M, Ito Y, Hagihara K, Inari M, Kita A, Fukao A, Fujiwara T, Hirai S, Tani T, Sugiura R (2017) Spatial regulation of the KH domain RNA-binding protein Rnc1 mediated by a Crm1-independent nuclear export system in Schizosaccharomyces pombe. Mol Microbiol 104:428–448. https://doi.org/10.1111/mmi.13636 CrossRefGoogle Scholar
- Soucek S, Zeng Y, Bellur DL, Bergkessel M, Morris KJ, Deng Q, Duong D, Seyfried NT, Guthrie C, Staley JP, Fasken MB, Corbett AH (2016) The evolutionarily-conserved polyadenosine RNA binding protein, Nab2, cooperates with splicing machinery to regulate the fate of pre-mRNA. Mol Cell Biol 36:2697–2714. https://doi.org/10.1128/MCB.00402-16 CrossRefGoogle Scholar