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Current Genetics

, Volume 64, Issue 5, pp 1071–1087 | Cite as

Cell size is regulated by phospholipids and not by storage lipids in Saccharomyces cerevisiae

  • Monala Jayaprakash Rao
  • Malathi Srinivasan
  • Ram Rajasekharan
Original Article

Abstract

Cell size and morphology are key adaptive features that influence almost all aspects of cellular physiology such as cell cycle and lipid metabolism. Here we report the role of a transcription factor Suppressor Phenotype of Ty elements insertion 10 (SPT10) of Saccharomyces cerevisiae in regulating cell cycle, cell size and lipid metabolism in concert, in addition to its defined role of histone gene expression. Morphological and biochemical analyses of spt10Δ strain show an abnormal cell size, cell cycle and lipid levels. The expression of Spt10p in spt10Δ strain helps the cell revert to typical wild-type phenotypes. SPT10 controls lipid metabolism by negatively regulating the expression of lipid biosynthetic genes, and positively regulating the expression of the lipid hydrolyzing genes. Spt10p helps in maintaining the cell size by regulating the amount of carbon flux into the phospholipid constituents of the cell membranes. On the contrary, storage lipids have no role in regulating the cell size. An exogenous supply of phosphatidic acid increases the cell size, proving the positive impact of the phospholipids on cell size modulation. SPT10 affects cell cycle, cell size and lipid metabolism by an orchestrated transcriptional regulation of the corresponding genes.

Keywords

SPT10 Phosphatidic acid Phosphatidylcholine Phosphatidylethanolamine Cell size Lipid metabolism H1246 RH1246 

Notes

Acknowledgements

This study was supported by the Council of Scientific and Industrial Research (CSIR), New Delhi, under the 12th 5-year plan project LIPIC (BSC0401). M. Jayaprakash Rao was supported by a fellowship from CSIR, New Delhi. The corresponding author is a recipient of the JC Bose national fellowship. We are grateful to the Department of Biochemistry, Indian Institute of Science, and C-CAMP, Bangalore for help with the radioactive study and Cell sorting facility, respectively.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

294_2018_821_MOESM1_ESM.docx (871 kb)
Supplementary material 1 (DOCX 871 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Lipid Science, Lipidomics CenterCSIR-Central Food Technological Research InstituteMysoreIndia
  2. 2.Academy of Scientific and Innovative ResearchCSIR-Central Food Technological Research InstituteMysoreIndia

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