This study designed to investigate the properties of antibacterial nanofiber scaffolds of polyurethane-Cinnamomum zeylanicum against virulence gene expression inhibition of Pseudomonas aeruginosa and Staphylococcus aureus that are important in burn wounds. With attention to burn wound infections in hospitals and mortality increase in patients, it is necessary to design nanodressing. Clinical isolates were confirmed by biochemical and microbiological tests. DNA of isolates was extracted and PCR used to confirm the alp gene of P. aeruginosa and Pv gene of S. aureus. Polyurethane nanofiber and cinnamon polymers were used to prepare the scaffold under the electrospinning process. Infrared spectroscopy, electron microscopy, and mechanical tensile tests were used to confirm the scaffolds. The susceptibility testing and minimum inhibitory concentration of polyurethane-cinnamon nanofiber scaffold were determined against P. aeruginosa and S. aureus. For confirmation of polyurethane-cinnamon nanofiber scaffold were used the cytotoxicity test (MTT), FTIR, mechanical tensile test, and a scanning electron microscope. The expression of virulence genes was investigated using the real-time RT-PCR technique. The results of the susceptibility testing indicated that P. aeruginosa and S. aureus were susceptible to polyurethane-cinnamon nanofiber scaffold. The MTT, FTIR, mechanical tensile test, and SEM confirmed the different features of the polyurethane-cinnamon nanofiber scaffold. Results of real-time PCR demonstrated that the expression levels of p–v and alp genes after treatment decreased, respectively, 2.71- and 1.06-fold. Results indicated that the electrospun polyurethane-cinnamon nanofiber scaffold for the first time could inhibit both important pathogens of the hospital and the expression of the virulence genes. Considering the susceptibility of P. aeruginosa and S. aureus to and its inhibitory effect on an alp and p–v genes, this system could probably be a candidate in wound dressing for commercial purposes to burn healing and infection inhibition.
This is a preview of subscription content, log in to check access.
Buy single article
Instant unlimited access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Availability of data and materials
The datasets analyzed during the current study are available from the corresponding author on reasonable request.
Urbanowski ML, Lykken GL, Yahr TL (2005) A secreted regulatory protein couples transcription to the secretory activity of the Pseudomonas aeruginosa type III secretion system. Proc Natl Acad Sci 102:9930–9935
Japoni A, Alborzi A, Kalani M, Nasiri J, Hayati M, Farshad S (2006) Susceptibility patterns and cross-resistance of antibiotics against Pseudomonas aeruginosa isolated from burn patients in the South of Iran. Burns 32:343–347
Barbieri J, Sun J (2004) Pseudomonas aeruginosa exoS and exoT. In: Cordat E, Barber D, Leipziger J, Pardo L, Stock C, Schmitt N, O’Donnell ME (eds) Reviews of physiology, biochemistry, and pharmacology. Springer, New York, pp 79–92
Hosseini SMJ, Naeini NS, Khaledi A, Daymad SF, Esmaeili D (2016) Evaluate the relationship between class 1 integrons and drug resistance genes in clinical isolates of Pseudomonas aeruginosa. Open Microbiol J 10:188
Abbasi A, Maddah SM, Mahboubi A, Khaledi A, Vazini H, Esmaeili D (2017) Investigate the inhibitory effects of Satureja khuzestanica essential oil against housekeeping fabD and exoA genes of Pseudomonas aeruginosa from Hospital Isolates using RT-PCR technique. Ann Med Health Sci Res
Karatuna O, Yagci A (2010) Analysis of quorum sensing-dependent virulence factor production and its relationship with antimicrobial susceptibility in Pseudomonas aeruginosa respiratory isolates. Clin Microbiol Infect 16:1770–1775
Arora D, Jindal N, Kumar R, Romit M (2011) Emerging antibiotic resistance in Pseudomonas-A challenge. Int J Pharm Pharm Sci 3:82–84
Moore NM, Flaws ML (2011) Antimicrobial resistance mechanisms in Pseudomonas aeruginosa. Clin Lab Sci 24:47
Bowersox J (1999) Experimental staph vaccine broadly protective in animal studies. NIH News 27
Khademi F, Ghanbari F, Mellmann A, Najafzadeh MJ, Khaledi A (2016) Phylogenetic relationships among Staphylococcus aureus isolated from clinical samples in Mashhad, Iran. J Infect Public Health 9:639–644
Argudín MÁ, Mendoza MC, Rodicio MR (2010) Food poisoning and Staphylococcus aureus enterotoxins. Toxins 2:1751–1773
Jayaprakasha GK, Jagan Mohan Rao L, Sakariah KK (2003) Volatile constituents from Cinnamomum zeylanicum fruit stalks and their antioxidant activities. J Agric Food Chem 51:4344–4348
Du W-X, Avena-Bustillos RJ, Woods R, Breksa AP, McHugh TH, Friedman M et al (2012) Sensory evaluation of baked chicken wrapped with antimicrobial apple and tomato edible films formulated with cinnamaldehyde and carvacrol. J Agric Food Chem 60:7799–7804
Todd J, Friedman M, Patel J, Jaroni D, Ravishankar S (2013) The antimicrobial effects of cinnamon leaf oil against multi-drug resistant Salmonella Newport on organic leafy greens. Int J Food Microbiol 166:193–199
Li M, Guo Y, Wei Y, MacDiarmid AG, Lelkes PI (2006) Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications. Biomaterials 27:2705–2715
Agarwal S, Wendorff JH, Greiner A (2008) Use of electrospinning technique for biomedical applications. Polymer 49:5603–5621
Lu P, Ding B (2008) Applications of electrospun fibers. Recent Patents Nanotechnol 2:169–182
You Y, Lee SJ, Min BM, Park WH (2006) Effect of solution properties on the nanofibrous structure of electrospun poly (lactic-co-glycolic acid). J Appl Polym Sci 99:1214–1221
Metzke M, O’Connor N, Maiti S, Nelson E, Guan Z (2005) Saccharide–peptide hybrid copolymers as biomaterials. Angew Chem 117:6687–6691
Pham QP, Sharma U, Mikos AG (2006) Electrospinning of polymeric nanofibers for tissue engineering applications: a review. Tissue Eng 12:1197–1211
Duan B, Yuan X, Zhu Y, Zhang Y, Li X, Zhang Y et al (2006) A nanofibrous composite membrane of PLGA–chitosan/PVA prepared by electrospinning. Eur Polym J 42:2013–2022
Kenawy E-R, Bowlin GL, Mansfield K, Layman J, Simpson DG, Sanders EH et al (2002) Release of tetracycline hydrochloride from electrospun poly(ethylene-co-vinyl acetate), poly (lactic acid), and a blend. J Control Rel 81:57–64
Wang S, Zhang Y, Wang H, Yin G, Dong Z (2009) Fabrication and properties of the electrospun polylactide/silk fibroin-gelatin composite tubular scaffold. Biomacromolecules 10:2240–2244
Patel JB, Tenover FC, Turnidge JD, Jorgensen JH (2011) Susceptibility test methods: dilution and disk diffusion methods. In: Carroll KC, Pfaller MA, Landry ML, McAdam AJ, Patel R, Richter SS, Warnock DW (eds) Manual of clinical microbiology, 10th edn. American Society of Microbiology, Washington, pp 1122–1143
Wayne P (2007) Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing, p 17
Yousefzadi M, Riahi-Madvar A, Hadian J, Rezaee F, Rafiee R, Biniaz M (2014) Toxicity of essential oil of Satureja khuzistanica: in vitro cytotoxicity and anti-microbial activity. J Immunotoxicol 11:50–55
Hiep NT, Lee B-T (2010) Electro-spinning of PLGA/PCL blends for tissue engineering and their biocompatibility. J Mater Sci Mater Med 21:1969–1978
Aoki S, Hirakata Y, Kondoh A, Gotoh N, Yanagihara K, Miyazaki Y et al (2004) Virulence of Metallo-β-lactamase-producing Pseudomonas aeruginosa in vitro and in vivo. Antimicrob Agents Chemother 48:1876–1878
Hasan A, Memic A, Annabi N, Hossain M, Paul A, Dokmeci MR et al (2014) Electrospun scaffolds for tissue engineering of vascular grafts. Acta Biomater 10:11–25
Zhang Y, Liu X, Wang Y, Jiang P, Quek S (2016) Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Food Control 59:282–289
Vasita R, Katti DS (2006) Nanofibers and their applications in tissue engineering. Int J Nanomed 1:15
Diaz-Gomez L, Alvarez-Lorenzo C, Concheiro A, Silva M, Dominguez F, Sheikh FA et al (2014) Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation. Mater Sci Eng C 40:180–188
Nanstad R, McCabe D, Swain R, Miller M (1992) Chemical composition and RT NDT determinations for Midland weld WF-70. Nuclear Regulatory Commission
Oussalah M, Caillet S, Saucier L, Lacroix M (2007) Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157: H7, Salmonella typhimurium, Staphylococcus aureus, and Listeria monocytogenes. Food Control 18:414–420
Abbasi A, Bahador A, Esmaeili D, Mahbubi A, Amiri M, Amiri M (2014) The study of inhibitory effects of Satureja khuzestanica against MDR isolates of pseudomonas aeruginosa. Int J Curr Microbiol Appl Sci 3:614–618
Zainab Waheed ZA, Dong Y, Han N, Liu S (2019) Data for holographic polymer nanocomposites with ordered structures and improved electro-optical performance by doping POS. Compos B 174
Zinatloo-Ajabshir S, Salehi Z, Amiri O, Salavati-Niasari M (2019) Simple fabrication of Pr2Ce2O7 nanostructures via a new and eco-friendly route; a potential electrochemical hydrogen storage material. J Alloys Compd 791(30):792–799
Zinatloo-Ajabshir S, SadatMorassaei M, Salavati-Niasari M (2019) Simple approach for the synthesis of Dy2Sn2O7 nanostructures as a hydrogen storage material from banana juice. J Clean Prod 222:103–110
Wang Y, ShiPan M, Zhong S, Wu Y, Yang R, Han Y, Zhou J (2017) Combined ANFIS and numerical methods to simulate ultrasound-assisted extraction of phenolics from chokeberry cultivated in China and analysis of phenolic composition. Technology 178:178–188
Esmaeilli D, Mobarez AM, Salmanian AH, Hosseini AZ (2013) Bioactivity and immunological evaluation of LPS from different serotypes of Helicobacter pylori. Iran J Microbiol 5(2):142
We would like to thank our colleagues at BMSU, for their help.
This work was supported by the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Hosseinpor, H., Khaledi, A. & Esmaeili, D. The properties of nanofiber scaffolds of polyurethane-Cinnamomum zeylanicum against pathogens of Pseudomonas aeruginosa and Staphylococcus aureus. Polym. Bull. (2020). https://doi.org/10.1007/s00289-019-03095-1