Preparation of multitarget Fusarium toxin (zearalenone, deoxynivalenol, T-2, and HT-2) immunoaffinity columns using poly(glycidyl methacrylate–divinylbenzene) as a matrix

  • Huijun Liu
  • Jianing Zhang
  • Ke DingEmail author
  • Xiangning Chen
  • Tao Han
Original Paper


This study aimed to establish a purifying immunoaffinity column (IAC) for four kinds of Fusarium toxins (zearalenone, deoxynivalenol, T-2, and HT-2) simultaneously using poly-glycidyl methacrylate–divinylbenzene [P(GMA–DVB)] as the column matrix. The P(GMA–DVB) microsphere was synthesized using glycidyl methacrylate (GMA) and diethylbenzene (DVB) at an optimal polymerization temperature of 80 °C, the initiator 2,2′-azodiisobutyronitrile concentration of 2.50 g/mL H2O, and the DVB-to-GMA ratio of 3:5 (wt.). The polymerized P(GMA–DVB) microspheres were modified with 1,6-hexanediamine, activated with glutaraldehyde, and coupled with the four toxin monoclonal antibodies. The coupling rate of the activated resin was calculated to be 95.95%. The maximum capacity of the IACs was 264 ng deoxynivalenol, 231 ng T-2, 210 ng HT-2, and 292 ng zearalenone. After eight uses, the maximum capacity had no obvious decrease. The recovery of the four Fusarium toxins on the P(GMA–DVB) IAC was 70.03–85.39% in flour, with the relative standard deviations of 4.67–15.93%. The IACs were applied to the actual flour samples.


Fusarium toxins Glycidyl methacrylate–divinylbenzene Immunoaffinity columns 



This study was funded by the Beijing Natural Science Fund (KZ201710020014), the National Natural Science Foundation of China (31601658), and the Beijing Natural Science Fund (14L00184).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Food Science and Engineering, Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide ResidueBeijing University of AgricultureBeijingChina

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