Kafirin-derived films for sustainable development by amidation and esterification

  • Umesh R. Mahajan
  • Shashank T. MhaskeEmail author
Original Paper


The current study represents the modification of the side-chain functionality of kafirin to prepare amide and ester derivatives. To explore the effect of the pendant group on kafirin protein, the derivatives were subjected to film formation by compression molding. Mechanical and thermal behaviors of these films were investigated. FTIR and NMR confirmed the formation of amide and ester derivatives. Amide-derived films showed a reduction in the water absorption capacity as compared to the ester-derived films. The mechanical properties of amide-derived films were higher than that of unmodified kafirin films due to the high proportion of grafted pendant groups. Kafirin treated with benzoyl chloride system has the highest tensile modulus as compared to the other modification. These findings suggest that the kafirin has the potential to be used as a packaging material in various sectors.

Graphic abstract


Kafirin Amidation Esterification compression molding Mechanical properties Thermal properties 


Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

289_2019_2876_MOESM1_ESM.docx (638 kb)
Supplementary material 1 (DOCX 638 kb)


  1. 1.
    Krochta JM (2002) Proteins as raw materials for films and coatings: definitions, current status and opportunities. In: Gennadios A (ed) Protein-based films and coatings. CRC Press, Boca RatonGoogle Scholar
  2. 2.
    Gao C, Taylor J, Wellner N, Yaruhanga Y, Parker M, Mills E, Belton P (2005) Effect of preparation conditions on protein secondary structure and biofilm formation of kafirin. J Agric Food Chem 53:306–312CrossRefGoogle Scholar
  3. 3.
    Torres-Giner S, Gimenez E, Largaron J (2008) Characterization of the morphology and thermal properties of zein prolamin nanostructure obtained by electrospinning. Food Hydrocoll 22:601–614CrossRefGoogle Scholar
  4. 4.
    Taylor J, Belton P, Minaar A (2009) Formation of kafirin microparticles by phase separation from an organic acid and their characterization. J Cereal Sci 50:99–105CrossRefGoogle Scholar
  5. 5.
    Hiks K (2005) Encapsulation of essential oils in zein nano spherical particles. J Agric Food Chem 53:4788–4792CrossRefGoogle Scholar
  6. 6.
    Gong S, Wang H, Sun Q, Xue S, Wang J (2006) Mechanical properties and in vitro biocompatibility of porous zein scaffolds. Biomaterials 27:3793–3799CrossRefGoogle Scholar
  7. 7.
    Erickson D, Campanella O, Hamaker B (2012) Functionalizing maize zein in viscoelastic dough system through fibrous, β- Sheet rich protein network: an alternative, physicochemical approach to gluten-free breadmaking trends. Food Sci Technol 24:74–81CrossRefGoogle Scholar
  8. 8.
    Perez C, Regaldo-gonz C, Rogriguez C, Barbosa J (2006) Incorporation of antimicrobial agents in food packaging films and coating. Adv Agric Food Biotechnol 37:193–216Google Scholar
  9. 9.
    Buchner S, Kinnear M, Crouch I, Taylor J, Minnaar A (2011) Extending the post-harvest sensory quality and shelflife of ‘Packham’s Triumph’ pears with a kafirin protein coating. J Sci Food Agric 91:2814–2820CrossRefGoogle Scholar
  10. 10.
    Valerini D, Tammaro L, Di Benedetto F (2018) Aluminum-doped zinc coatings on polylactic acid films for antimicrobial food packaging. Thin Solid Films 645:187–192CrossRefGoogle Scholar
  11. 11.
    Briassoulis D, Giannoulis A (2018) Evaluation of Biobased plastic mulching films. J Polym Test 67:99–109CrossRefGoogle Scholar
  12. 12.
    Wu X, Zheng S, Daniel A, Aguilar B (2018) Transparent ice-phobic coatings using bio-based epoxy resin. Mater Design 140:516–523CrossRefGoogle Scholar
  13. 13.
    Soltani S, Zerafat M, Sabbaghi S (2018) A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications. Carbohydr Polym 189:48–55CrossRefGoogle Scholar
  14. 14.
    Cazóna P, Vázquezb M, Velazqueza G (2018) Cellulose-glycerol-polyvinyl alcohol composite films for food packaging: evaluation of water adsorption, mechanical properties, light-barrier properties and transparency. Carbohydr Polym 195:432–443CrossRefGoogle Scholar
  15. 15.
    Xiao J, Yunqi L, Aljandro P, Qiuyang X, Qingrong H (2014) Structure, morphology and assembly behavior of kafirin. J Agric Food Chem 63:216–224CrossRefGoogle Scholar
  16. 16.
    Belton P, Delgadillo I, Halford N, Shewry P (2006) Kafirin structure and functionality. J Cereal Sci 44(3):272–286CrossRefGoogle Scholar
  17. 17.
    Taylor J, Anyango J (2013) Developments in the science of zein, kafirin and gluten protein bio-plastic materials. Cereal Chem 90:344–357CrossRefGoogle Scholar
  18. 18.
    Raza H, Pasha I, Shoib M, Zaaboul F, Niazi S, Aboshora W (2017) Review on functional and rheological attributes of kafirin for utilization in the gluten-free baking industry. Am J Food Sci Nutr Res 4:150–157Google Scholar
  19. 19.
    De Mesa-Stonestreet N, Alavi S, Bean S (2010) Sorghum proteins: the concentration, isolation, modification, and food application of kafirins. J Food Sci 75(5):R90–R104Google Scholar
  20. 20.
    Xiao J, Chen Y, Huang Q (2017) Physicochemical properties of kafirin protein and its applications as building blocks of functional delivery systems. Food Funct. Google Scholar
  21. 21.
    Fountoulakis M, Lahm H (1998) Hydrolysis and amino acid composition analysis of protein. J Chromatogr A 826(2):109–134CrossRefGoogle Scholar
  22. 22.
    Zean Acetate (1941) Collins Veatch, La Grange III, assigner to corn product refining company, New York, NY. A corporation of New Jersey, Serial no. 2459822, US PATENT2236768Google Scholar
  23. 23.
    Biswas A, Sessa D, Lawton J, Gordon S, Willett J (2005) Microwave-assisted rapid modification of zein by octenyl succinic anhydride. Cereal Chem 82:1–3CrossRefGoogle Scholar
  24. 24.
    Sessa D, Cheng H, Kim S, Selling G, Biswas A (2013) Zein based polymers formed by modifications with isocyanates. Ind Crops Prod 43:106–113CrossRefGoogle Scholar
  25. 25.
    Yin H, Lu T, Liu L, Lu C (2014) Preparation, characterization and application of a novel biodegradable macromolecule: carboxymethyl. Int J Biol Macromol. Google Scholar
  26. 26.
    Qiangxian W, Tomoyuki Y, Hiroshi S, Hongkang Z, Seiichiro I (2003) Chemical modification of zein by bifunctional polycaprolactone. Polymer 44:3909–3919CrossRefGoogle Scholar
  27. 27.
    Emmambux M, Stading M, Taylor J (2004) Sorghum kafirin film property modification with hydrolyzable and condensed tannins. J Cereal Sci 40:127–135CrossRefGoogle Scholar
  28. 28.
    Byarhanga Y, Emmambux M, Wellner N, Ng KG, Taylor J (2006) Alteration of kafirin and kafirin film structure by heating with microwave energy and tannin complexation. J Agric Food Chem 54:4198–4207CrossRefGoogle Scholar
  29. 29.
    Olivera N, Roufa T, Bonillaa J, Carriazob J, Dianda N, Kokinia J (2019) Effect of LAPONITE® addition on the mechanical, barrier and surface properties of novel biodegradable kafirin nanocomposite films. J Food Eng 245:24–32CrossRefGoogle Scholar
  30. 30.
    Beckwith A, Wall J, Dimler R (1963) Amino groups as interaction sites in wheat gluten proteins: effect of amide-ester conversion. Arch Biochem Biophys 103:319–330CrossRefGoogle Scholar
  31. 31.
    Biswas A, Sessa DJ, Gordon SH, Lawton JW, Willett JL (2005) Synthesis of zein derivatives and their mechanical properties. Polym biocatal biomater. Google Scholar
  32. 32.
    Padua G, Lai H, Geil P (1999) X-ray diffraction characterization of structure of zein-oleic acid films. J Appl Polym Sci 71:1267–1281CrossRefGoogle Scholar
  33. 33.
    Park S, Baker J, Himmel M, Parilla P, Johnson D (2010) Cellulose crystallinity index: measurement technique and their impact on interpreting cellulose performance. Biotechnol Biofuel 3:10CrossRefGoogle Scholar
  34. 34.
    Xiao J, Chen Y, Huang Q (2017) Physicochemical properties of kafirin protein and its applications as building blocks of functional delivery systems. Food Funct. Google Scholar
  35. 35.
    Anyango J, Taylor J, Taylor J (2013) Role of γ-kafirin in the formation and organization of kafirin microstructures. J Agric Food Chem 61:10757–10765CrossRefGoogle Scholar
  36. 36.
    Matveev Y, Grinberg V, Tolstoguzov V (2000) The plasticizing effect of water on proteins, polysaccharides and their mixtures: glassy state of biopolymer. Food Seed Food Hydrocoll 14:425–437CrossRefGoogle Scholar
  37. 37.
    Reddy N, Tan Y, Li Y (2008) Effect of glutaraldehyde cross-linking condition on the strength and water stability of wheat gluten fibers. Macromol Mater Eng 293:614–620CrossRefGoogle Scholar
  38. 38.
    Ringe D, Petsko G (2003) The glass transition on protein dynamics: what is it, why it occurs and how to exploit it. Biophys Chem 105:667–680CrossRefGoogle Scholar
  39. 39.
    Brostow W, Chiu R, Kalogerqas IM, Vassilikou-Dova A (2008) Prediction of glass transition temperature, binary blends and copolymers. Mater Lett 62:3152–3155CrossRefGoogle Scholar
  40. 40.
    Wang Y, Tilley M, Bean S, Sun X, Wang D (2009) Comparision of methods for extracting kafirin protein from sorghum distillers dried grains with solubles. J Agric Food Chem 57:8366–8372CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Polymer and Surface EngineeringInstitute of Chemical TechnologyMumbaiIndia

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