The potential use of cross-linked alginate/gelatin hydrogels containing silver nanoparticles for wound dressing applications
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In this study, alginate/gelatin (Alg/Gel) hydrogels containing silver nanoparticles (AgNPs) cross-linked with 1.0, 2.5, and 5.0% w/v calcium chloride (CaCl2) were fabricated using solvent casting method. The morphology and mechanical properties of Alg/Gel hydrogels containing AgNPs were observed by scanning electron microscopy and universal testing machine. The mechanical performance demonstrated that the Alg/Gel hydrogels containing AgNPs cross-linked with CaCl2 showed improved mechanical properties with increasing CaCl2 concentration. The water swelling and weight loss behaviors of these hydrogels were investigated in an acetate buffer solution. Increasing concentration of CaCl2 from 1.0 to 5.0% w/v, the hydrogels manifested the lower swelling and weight loss behaviors. For in vitro AgNP release, the results showed that the released silver (Ag+) ions amount from the hydrogels cross-linked with 5% w/v CaCl2 was the highest followed by those of 1.0% and 2.5% w/v CaCl2, respectively. Moreover, these hydrogels showed the antibacterial activity against S. aureus, P. aeruginosa, and E. coli. Lastly, these hydrogels were non-toxic to both NHDF and NCTC clone 929 cells. Thus, these Alg/Gel hydrogels containing AgNPs had a potential for use in various wound dressing and biomedical fields.
KeywordsAlginate/gelatin hydrogel Silver nanoparticles Calcium chloride Wound dressing
This work was supported by Grant for International Research Integration: Research Pyramid, Ratchadaphiseksomphot Endowment Fund (GCURP_58_02_63_01) of Chulalongkorn University. The authors would like to thank the Scientific and Technological Instruments Center (STIC), Mae Fah Luang University and Chulabhorn Research Institute, Thailand, for laboratory facilities. Jittap Pranjan gratefully acknowledges the Research and Researchers for Industries-RRI M.Sc. scholarship (MSD60I0006) and MG Cordy Gold Co., Ltd. for financial support. Atchara Kaolaor would like to acknowledge the Research and Researchers for Industries-RRI M.Sc. scholarship (MSD60I0010) and Jinnaluck Co., Ltd. for financial support.
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