Fabrication of microcomposites based on silk sericin and monetite for bone tissue engineering
- 28 Downloads
In the present study, silk sericin–monetite microcomposites (SS–MM) were prepared using a simple and cost-effective method. Hemolytic assay was performed to determine the blood compatibility of SS–MM. MTT assay was carried out using MG-63 osteoblasts, to determine the cytocompatibility and proliferative efficacy of SS–MM. Alkaline phosphatase activity was studied to examine the osteogenic potency of the prepared microcomposites. The results obtained showed that the SS–MM can be effectively used as a bone graft for tissue engineering applications.
KeywordsBiomaterials Sericin Monetite Composite materials Osteoblast Bone graft
Silk sericin–monetite microcomposites
Scanning electron microscopy
Fourier transform infrared
Red blood cells
The authors greatly acknowledge the financial support received from the Chettinad Academy of Research and Education (CARE Fund) (Grant No. 2015-16) for carrying out the research work.
- 2.Ratner BD, Hoffman AS, Schoen FJ, Lemons JE (2012) Biomaterials science: an introduction to materials in medicine, 3rd edn. Academic Press, LondonGoogle Scholar
- 3.Bao C, Teo E, Chong M, Liu Y, Choolani M, Chan J (2013) Advances in bone tissue engineering. Regen Med Tissue Eng 599–614Google Scholar
- 12.Padol AR, Jayakumar K, Mohan K, Manochaya S (2012) Natural biomaterial silk and silk proteins: applications in tissue repair. Int J Mater Biomater Appl 2:19–24Google Scholar
- 17.Patel RJ, Modasiya MK (2011) Sericin: pharmaceutical applications. Int J Res Pharm Biomed Sci 2:913–917Google Scholar
- 27.Document ASTM (2005) Standard practice for assessment of hemolytic properties of materials designation: F 756-00. Annu Book ASTM Stand, pp 309–313Google Scholar
- 28.Liu Y, Cai D, Yang J, Wang Y, Zhang X, Yin S (2014) In vitro hemocompatibility evaluation of poly (4-hydroxybutyrate) scaffold. Int J Clin Exp Med 7:1233–1243Google Scholar