3D bioprinting of alginate scaffolds with controlled micropores by leaching of recrystallized salts

  • Xiaoyue Wei
  • Yongxiang LuoEmail author
  • Peng Huang
Original Paper


It is well known that three-dimensional scaffolds with controlled macropores and micropores are crucially important for tissue engineering. In the present study, a facile method based on leaching of recrystallized sodium chloride (NaCl) is used to create micropores in 3D printed alginate scaffolds. The macropores with size of 811 ± 78 µm were controlled by 3D printing, and the micropores with size of 3.2 ± 1.4 µm were produced by leaching of the recrystallized NaCl particles. The microporosity can be controlled by the added amount of sodium ions in the alginate inks. The properties of scaffolds including water adsorption, protein delivery and mechanical properties were tailored by the produced micropores. This simple and cell-friendly method might be interesting for 3D bioprinting of tissue engineering scaffolds with designed physical characteristics using alginate-based hydrogel bioinks.


Alginate scaffolds 3D printing Bioinks Micropores 



The Natural Science Foundation of China (Grant No. 81741107), Basic Research Program of Shenzhen (Grant Nos. JCYJ20170817094407954), Taipei University of Technology—Shenzhen University Joint Research Program (Grant No. 2018005) were granted.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science CenterShenzhen UniversityShenzhenChina

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