Prediction of breakthrough curves in packed-bed column as tool for lysozyme isolation using a green bed

  • M. Emilia Brassesco
  • Nadia Woitovich Valetti
  • Guillermo PicóEmail author
Original Paper


The adsorption of lysozyme onto alginate–guar gum bed cross-linked with epichlorohydrin has been studied in packed-bed column. Adsorption performance was evaluated with different bed heights, flow rates and initial protein concentrations. The Thomas and bed depth service time mathematical models fitted well the breakthrough curves experimental data with high correlation coefficients. Not modification of the working yield of the bed was observed in progressive ten cycles of reused. Lysozyme recovery of 75% was achieved from white egg with a purification factor around 15 under the condition of bed height 9 cm, flow rate 0.4 mL/min and total protein inlet 10 mg/mL, while the dynamic bed capacity under this experimental condition was 10.87 mg/g hydrated bed.


Alginate Guar gum Polyelectrolytes Lysozyme Breakthrough curves 



Guar gum










Alginate–guar gum bed



This work was supported by a grant from FonCyT, Project PICT 2013–271 – Argentina Innovator 2020. MEB is a fellowship from FonCyT.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • M. Emilia Brassesco
    • 1
  • Nadia Woitovich Valetti
    • 1
  • Guillermo Picó
    • 1
    Email author
  1. 1.Institute of Biotechnological and Chemistry Processes (IPROBYQ-CONICET) and Faculty of Biochemical and Pharmaceutical SciencesNational University of RosarioRosarioArgentina

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