The potential use of gentamicin sulfate-loaded poly(l-lactic acid)-sericin hybrid scaffolds for bone tissue engineering
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Poly(l-lactic acid) (PLLA) scaffolds were prepared by a particulate leaching method using sodium chloride (NaCl) with the size of 300–425 µm as a particulate leaching. The PLLA/NaCl weight ratios were varied to be 1:8, 1:10, 1:12, and 1:15. The gentamicin sulfate (GS)-loaded PLLA-sericin hybrid scaffolds were prepared by immersion of the PLLA scaffolds in sericin solution containing GS at a concentration of 5 mg mL−1 and subsequently freeze-drying. From the results, the pore sizes of the neat and the GS-loaded PLLA-sericin hybrid scaffolds ranged between 290 and 346 µm. The pore interconnectivity of these scaffolds increased with an increase in the amount of NaCl. Both the neat and the GS-loaded PLLA-sericin hybrid scaffolds that had been fabricated at PLLA/NaCl weight ratio of 1:15 showed high interconnected porous structure. Both the water retention and the weight loss increased with increasing NaCl content and submersion time. The increase in the porous structure of the scaffolds with the increasing NaCl content resulted in an observed decrease in the compressive modulus. Moreover, the cumulative released amounts of GS increased with increasing the porous structure of the scaffolds. All the GS-loaded PLLA-sericin hybrid scaffolds showed high activity against the growth of both E. coli TISTR 780 and S. aureus TISTR 1466. Lastly, all the GS-loaded PLLA-sericin hybrid scaffolds were proven non-toxic to MC3T3-E1 cells, indicating their potential uses for bone tissue engineering.
KeywordsPLLA scaffold Sericin Gentamicin sulfate Bone tissue engineering
The authors would like to acknowledge the financial support from Mae Fah Luang University (MFU). Porntipa Pankongadisak gratefully acknowledges the Royal Golden Jubilee Ph.D. scholarship (PHD/0094/2558), Thailand Research Fund (TRF). Kitipong Kiti also acknowledges the Royal Golden Jubilee Ph.D. scholarship (PHD/0043/2559), Thailand Research Fund (TRF).
- 9.Amini AR, Laurencin CT, Nukavarapu SP (2012) Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 40:363–408. https://doi.org/10.1615/CritRevBiomedEng.v40.i5.10 CrossRefGoogle Scholar
- 18.Puppi D, Chiellini F, Piras AM, Chiellini E (2010) Polymeric materials for bone and cartilage repair. Prog Polym Sci 35:403–440. https://doi.org/10.1016/j.progpolymsci.2010.01.006 CrossRefGoogle Scholar
- 19.Liu X, Ma PX (2004) Polymeric scaffolds for bone tissue engineering. Ann Biomed Eng 32:477–486. https://doi.org/10.1023/B:ABME.0000017544.36001.8e CrossRefGoogle Scholar
- 23.Doğan A, Demirci S, Bayir Y, Halici Z, Karakus E, Aydin A, Cadirci E, Albayrak A, Demirci E, Karaman A, Ayan AK, Gundogdu C, Şahin F (2014) Boron containing poly-(lactide-co-glycolide) (PLGA) scaffolds for bone tissue engineering. Mater Sci Eng C 44:246–253. https://doi.org/10.1016/j.msec.2014.08.035 CrossRefGoogle Scholar
- 34.Padamwar MN, Pawar AP (2004) Silk sericin and its applications: a review. J Sci Ind Res 63:323–329Google Scholar
- 38.Rocha LKH, Favaro LIL, Rios AC, Silva EC, Silva WF, Stigliani TP, Guilger M, Lima R, Oliveira JM Jr, Aranha N, Tubino M, Vila MMDC, Balcão VM (2017) Sericin from Bombyx mori cocoons. Part I: extraction and physicochemical-biological characterization for biopharmaceutical applications. Process Biochem 16:163–177. https://doi.org/10.1016/j.procbio.2017.06.019 CrossRefGoogle Scholar
- 48.Flores C, Degoutin S, Chai F, Raoul G, Hornez J-C, Martel B, Siepmann J, Ferri J, Blanchemain N (2016) Gentamicin-loaded poly(lactic-co-glycolic acid) microparticles for the prevention of maxillofacial and orthopedic implant infections. Mater Sci Eng C 64:108–116. https://doi.org/10.1016/j.msec.2016.03.064 CrossRefGoogle Scholar
- 49.Frutos P, Diez-Peña E, Frutos G, Barrales-Rienda JM (2002) Release of gentamicin sulphate from a modified commercial bone cement. Effect of (2-hydroxyethyl methacrylate) comonomer and poly(N-vinyl-2-pyrrolidone) additive on release mechanism and kinetics. Biomaterials 23:3787–3797. https://doi.org/10.1016/S0142-9612(02)00028-5 CrossRefGoogle Scholar
- 50.Aquino RP, Auriemma G, Mencherini T, Russo P, Porta A, Adami R, Liparoti S, Porta GD, Reverchon E, Gaudio PD (2013) Design and production of gentamicin/dextrans microparticles by supercritical assisted atomisation for the treatment of wound bacterial infections. Int J Pharm 440:188–194. https://doi.org/10.1016/j.ijpharm.2012.07.074 CrossRefGoogle Scholar
- 57.Deng Y, Zhang M, Chen X, Pu X, Liao X, Huang Z, Yin G (2017) A novel akermanite/poly (lactic-co-glycolic acid) porous composite scaffold fabricated via a solvent casting-particulate leaching method improved by solvent self-proliferating process. Regen Biomater 4:233–242. https://doi.org/10.1093/rb/rbx014 CrossRefGoogle Scholar
- 61.Chen Y, Zhou S, Li Q (2011) Microstructure design of biodegradable scaffold and its effect on tissue regeneration. Biomaterials 32:5003–5014. https://doi.org/10.1016/j.biomaterials.2011.03.064 CrossRefGoogle Scholar