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Preparation, characterization and in vitro biological study of silk fiber/methylcellulose composite for bone tissue engineering applications

  • Valarmathi Narayanan
  • Shanmugam Sumathi
Original Paper
  • 3 Downloads

Abstract

In the present work, silk fiber (SF) and methylcellulose (MC) composites were fabricated by solvent casting method and characterized in detail. The interactions between SF/MC composites were studied in detail by Fourier transform infrared (FT-IR) spectroscopy and powder X-ray diffraction (XRD). The surface morphology and thermal stability were studied. Viscosity, thickness, folding endurance, tensile strength and antioxidant activity were analyzed for different ratios of SF/MC composite. Antimicrobial activity, in vitro biomimetic mineralization, hemocompatibility and cell viability of the SF/MC composite were studied. The deposition of calcium and phosphorus ions from simulated body fluid (SBF) onto SF/MC composite surface was evidenced from XRD, FT-IR and SEM–EDS. Inductively coupled plasma-optical emission spectrometry analysis (ICP-OES) was utilized to analyze leaching of Ca and P ions from the SBF. Hemolytic assay proves that the composites were compatible with blood and hemolytic ratio is found to be less than 5%. The MTT assay test against MG-63 suggests that the SF/MC composites are promising biomaterials for bone tissue engineering applications.

Keywords

Silk fiber Methylcellulose Antioxidant activity Antimicrobial activity Hemocompatibility Biocompatibility 

Notes

Acknowledgements

We would like to thank VIT, Vellore, for providing all required facilities to carry out the work and IIT Madras for ICP-OES analysis.

References

  1. 1.
    Voicu SI, Condruz RM, Mitran V, Cimpean A, Miculescu F, Andronescu C, Miculescu M, Thakur VK (2016) Sericin covalent immobilization onto cellulose acetate membrane for biomedical applications. ACS Sustain Chem Eng.  https://doi.org/10.1021/acssuschemeng.5b01756 CrossRefGoogle Scholar
  2. 2.
    Miculescu F, Maidaniuc A, Voicu SI, Thakur VK, Stan GE, Ciocan LT (2017) Progress in hydroxyapatite-starch based sustainable biomaterials for biomedical bone substitution applications. ACS Sustain Chem Eng 5:8491–8512.  https://doi.org/10.1021/acssuschemeng.7b02314 CrossRefGoogle Scholar
  3. 3.
    Sonia Mandal BB, Kapoor KS (2009) Silk fibroin/polyacrylamide semi-interpenetrating network hydrogels for controlled drug release. Biomaterials 30:2826–2836.  https://doi.org/10.1016/j.biomaterials.2009.01.040 CrossRefGoogle Scholar
  4. 4.
    Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL (2003) Silk-based biomaterials. Biomaterials 24:401–416.  https://doi.org/10.1016/S0142-9612(02)00353-8 CrossRefGoogle Scholar
  5. 5.
    He J, Guo N, Cui S (2011) Structure and Mechanical properties of electrospun tussah silk fibroin nanofibres: variations in processing parameters. Iran Polym J 20:713–724Google Scholar
  6. 6.
    Mirahmadi F, Shadpour MT, Shokrgozar MA, Bonakdar S (2013) Enhanced mechanical properties of thermosensitive chitosan hydrogel by silk fibers for cartilage tissue engineering. Mater Sci Eng, C 33:4786–4794.  https://doi.org/10.1016/j.msec.2013.07.043 CrossRefGoogle Scholar
  7. 7.
    Iwamoto S, Isogai A, Iwata T (2011) Structure and mechanical properties of wet-spun fibers made from natural cellulose nanofibers. Biomacromol 12:831–836.  https://doi.org/10.1021/bm101510r CrossRefGoogle Scholar
  8. 8.
    Pandele AM, Neacsu P, Cimpean A, Staras AI, Miculescu F, Iordache A, Voicu SI, Thakur VK, Toader OD (2018) Cellulose acetate membranes functionalized with resveratrol by covalent immobilization for improved osseointegration. Appl Surf Sci 438:2–13.  https://doi.org/10.1016/j.apsusc.2017.11.102 CrossRefGoogle Scholar
  9. 9.
    Pandele AM, Comanici FE, Carp CA, Miculescu F, Voicu SI, Thakur VK, Serban BC (2017) Synthesis and characterization of cellulose acetate-hydroxyapatite micro and nano composites membranes for water purification and biomedical applications. Vacuum 146:599–605.  https://doi.org/10.1016/j.vacuum.2017.05.008 CrossRefGoogle Scholar
  10. 10.
    Li G, Li Y, Chen G, He J, Han Y, Wang X, Kaplan DL (2015) Silk-based biomaterials in biomedical textiles and fiber-based implants. Adv Healthc Mater 4:1134–1151.  https://doi.org/10.1002/adhm.201500002 CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Bhardwaj N, Kundu SC (2011) Silk fibroin protein and chitosan polyelectrolyte complex porous scaffolds for tissue engineering applications. Carbohydr Polym 85:325–333.  https://doi.org/10.1016/j.carbpol.2011.02.027 CrossRefGoogle Scholar
  12. 12.
    Cai ZX, Mo XM, Zhang KH, Fan LP, Yin AL, He CL, Wang HS (2010) Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications. Int J Mol Sci 11:3529–3539.  https://doi.org/10.3390/ijms11093529 CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Nonsee K, Supitchaya C, Thawien W (2011) Antimicrobial activity and the properties of edible hydroxypropyl methylcellulose based films incorporated with encapsulated clove (Eugenia caryophyllata Thunb.) oil. Int Food Res J 18:1531–1541Google Scholar
  14. 14.
    Jiang H, Zuo Y, Zou Q, Wang H, Du J, Li Y, Yang X (2013) Biomimetic spiral-cylindrical scaffold based on hybrid chitosan/cellulose/nano-hydroxyapatite membrane for bone regeneration. ACS Appl Mater Interfaces 5:12036–12044.  https://doi.org/10.1021/am4038432 CrossRefPubMedGoogle Scholar
  15. 15.
    Diba M, Fathi MH, Kharaziha M (2011) Novel forsterite/polycaprolactone nanocomposite scaffold for tissue engineering applications. Mater Lett 65:1931–1934.  https://doi.org/10.1016/j.matlet.2011.03.047 CrossRefGoogle Scholar
  16. 16.
    Balan V, Verestiuc L (2014) Strategies to improve chitosan hemocompatibility: a review. Eur Polym J 53:171–188.  https://doi.org/10.1016/j.eurpolymj.2014.01.033 CrossRefGoogle Scholar
  17. 17.
    Ming J, Jiang Z, Wang P, Bie S, Zuo B (2015) Silk fibroin/sodium alginate fibrous hydrogels regulated hydroxyapatite crystal growth. Mater Sci Eng, C 51:287–293.  https://doi.org/10.1016/j.msec.2015.03.014 CrossRefGoogle Scholar
  18. 18.
    Hima Bindu TVL, Vidyavathi M, Kavitha K, Sastry TP, Suresh Kumar RV (2010) Preparation and evaluation of chitosan-gelatin composite films for wound healing activity. Trends Biomater Artif Organs 24:123–130Google Scholar
  19. 19.
    Rajesha Shetty G, Lakshmeesha Rao B, Asha S, Wang Y, Sangappa Y (2015) Preparation and characterization of silk fibroin/hydroxypropyl methyl cellulose (HPMC) blend films. Fibers Polym 16:1734–1741.  https://doi.org/10.1007/s12221-015-5223-z CrossRefGoogle Scholar
  20. 20.
    Anderson JM, Rodriguez A, Chang DT (2008) foreign body reaction to biomaterials. Semin Immunol 20:86–100.  https://doi.org/10.1016/j.smim.2007.11.004 CrossRefGoogle Scholar
  21. 21.
    Pachiappan P, Mohanraj P, Mahalingam CA, Manimegalai S, Swathiga G, Thangamalar A (2016) In vitro evaluation of antioxidant activity of bioproducts extracted from silkworm pupae. Environ We Int J Sci Technol 11:33–39Google Scholar
  22. 22.
    Radha G, Balakumar S, Venkatesan B, Vellaichamy E (2015) Evaluation of hemocompatibility and in vitro immersion on microwave-assisted hydroxyapatite–alumina nanocomposites. Mater Sci Eng, C 50:143–150.  https://doi.org/10.1016/j.msec.2015.01.054 CrossRefGoogle Scholar
  23. 23.
    Chandra VS, Baskar G, Suganthi RV, Elayaraja K, Joshy MI, Beaula WS, Mythili R, Venkatraman G, Kalkura SN (2012) Blood compatibility of iron-doped nanosize hydroxyapatite and its drug release. ACS Appl Mater Interfaces 4:1200–1210.  https://doi.org/10.1021/am300140q CrossRefPubMedGoogle Scholar
  24. 24.
    Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity. Biomaterials 27:2907–2915.  https://doi.org/10.1016/j.biomaterials.2006.01.017 CrossRefGoogle Scholar
  25. 25.
    Shao W, He J, Sang F, Ding B, Chen L, Cu S, Li K, Han Q, Tan W (2016) Coaxial electrospun aligned tussah silk fibroin nanostructured fiber scaffolds embedded with hydroxyapatite–tussah silk fibroin nanoparticles for bone tissue engineering. Mater Sci Eng, C 58:342–351.  https://doi.org/10.1016/j.msec.2015.08.046 CrossRefGoogle Scholar
  26. 26.
    More MP, Patil MD, Pandey AP, Patil PO, Deshmukh PK (2017) Fabrication and characterization of graphene-based hybrid nanocomposite: assessment of antibacterial potential and biomedical application. Artif Cells Nanomed Biotechnol.  https://doi.org/10.1080/21691401.2016.1252384 CrossRefPubMedGoogle Scholar
  27. 27.
    Shahzad S, Yar M, Siddiqi SA, Mahmood N, Rauf A, Qureshi ZU, Anwar MS, Afzaal S (2015) Chitosan-based electrospun nano-fibrous mats, hydrogels and cast films: novel anti-bacterial wound dressing matrices. J Mater Sci Mater Med 26:136.  https://doi.org/10.1007/s10856-015-5462-y CrossRefPubMedGoogle Scholar
  28. 28.
    Oliveira Barud HG, Barud Hda S, Cavicchioli M, do Amaral TS, de Oliveira Junior OB, Santos DM, Petersen AL, Celes F, Borges VM, de Oliveira CL, de Oliveira PF, Furtado RA, Tavares DC, Ribeiro SJ (2015) Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration. Carbohydr Polym 128:41–51.  https://doi.org/10.1016/j.carbpol.2015.04.007 CrossRefPubMedGoogle Scholar
  29. 29.
    Lee JM, Kim JH, Lee OJ, Park CH (2013) The fixation effect of a silk fibroin-bacterial cellulose composite plate in segmental defects of the zygomatic arch. JAMA Otolaryngol Head Neck Surg 139:629–635.  https://doi.org/10.1001/jamaoto.2013.3044 CrossRefPubMedGoogle Scholar
  30. 30.
    Hamad WY (2017) Cellulose Nanocrystals: Properties. Production and Applications, Book ChapterCrossRefGoogle Scholar
  31. 31.
    Samal SK, Dash M, Chiellini F, Wang X, Chiellini E, Declercq HA, Kaplan DL (2014) Silk/chitosan biohybrid hydrogels and scaffolds via green technology. RSC Adv 4:53547–53556.  https://doi.org/10.1039/C4RA10070K CrossRefGoogle Scholar
  32. 32.
    Nogueira GM, Weska FR, Vieira WC, Polakiewicz B, Rodas ACD, Higa OZ, Beppu MM (2009) A new method to prepare porous silk fibroin membranes suitable for tissue scaffolding applications. J Appl Polym Sci 114:617–623.  https://doi.org/10.1002/app.30627 CrossRefGoogle Scholar
  33. 33.
    Calamak S, Erdogdu C, Ozalp M, Ulubayram K (2014) Silk fibroin based antibacterial bionanotextiles as wound dressing materials. Mater Sci Eng, C 43:11–20.  https://doi.org/10.1016/j.msec.2014.07.001 CrossRefGoogle Scholar
  34. 34.
    Ludueña LN, Ponce A, Alvarez VA (2014) Poly(vinyl alcohol)/cellulose nanowhiskers nanocomposite hydrogels for potential wound dressings. Mater Sci Eng, C 34:54–61.  https://doi.org/10.1016/j.msec.2013.10.006 CrossRefGoogle Scholar
  35. 35.
    Shalumon KT, Anulekha KH, Nair SV, Nair SV, Chennazhi KP, Jayakumar R (2011) Sodium alginate/poly(vinyl alcohol)/nano ZnO composite nanofibers for antibacterial wound dressings. Int J Biol Macromol 49:247–254.  https://doi.org/10.1016/j.ijbiomac.2011.04.005 CrossRefPubMedGoogle Scholar
  36. 36.
    Li DW, Lei X, He FL, He J, Liu YL, Ye YJ, Deng X, Duanb E, Yin DC (2017) Silk fibroin/chitosan scaffold with tunable properties and low inflammatory response assists the differentiation of bone marrow mesenchymal stem cells. Int J Biol Macromol 105:584–597.  https://doi.org/10.1016/j.ijbiomac.2017.07.080 CrossRefPubMedGoogle Scholar
  37. 37.
    Chahal S, Hussain FSJ, Kumar A, Rasad MSBA, Yusoff MM (2016) Fabrication, characterization and in vitro biocompatibility of electrospun hydroxyethyl cellulose/poly (vinyl) alcohol nano-fibrous composite biomaterial for bone tissue engineering. Chem Eng Sci 144:17–29.  https://doi.org/10.1016/j.ces.2015.12.030 CrossRefGoogle Scholar
  38. 38.
    Singh BN, Panda NN, Mund R, Pramanik K (2016) Carboxymethyl cellulose enables silk fibroin nanofibrous scaffold with enhanced biomimetic potential for bone tissue engineering application. Carbohydr Polym 151:335–347.  https://doi.org/10.1016/j.carbpol.2016.05.088 CrossRefPubMedGoogle Scholar
  39. 39.
    Ariaii P, Tavakolipour H, Rezai M, Rad AHE (2014) Properties and antimicrobial activity of edible methylcellulose based film incorporated with Pimpinella affinis oil. Eur J Exp Biol 4:670–676Google Scholar
  40. 40.
    Sabaa MW, Abdallah HM, Mohamed NA, Mohamed RR (2015) Synthesis, characterization and application of biodegradable crosslinked carboxymethyl chitosan/poly(vinyl alcohol) clay nanocomposites. Mater Sci Eng, C 56:363–373CrossRefGoogle Scholar
  41. 41.
    Tan H, Wu B, Li C, Mu C, Li H, Lin W (2015) Collagen cryogel cross-linked by naturally derived dialdehyde carboxymethyl cellulose. Carbohydr Polym 129:17–24.  https://doi.org/10.1016/j.carbpol.2015.04.029 CrossRefGoogle Scholar
  42. 42.
    Li D, Ye Y, Li D, Li X, Mu C (2016) Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin–PEG composite hydrogel fibers for wound dressings. Carbohydr Polym 137:508–514.  https://doi.org/10.1016/j.carbpol.2015.11.024 CrossRefPubMedGoogle Scholar
  43. 43.
    Yin N, Chen SY, Ouyang Y, Tang L, Yang JX, Wang HP (2011) Biomimetic mineralization synthesis of hydroxyapatite bacterial cellulose nanocomposites. Prog Nat Sci Mater Int 21:472–477.  https://doi.org/10.1016/S1002-0071(12)60085-9 CrossRefGoogle Scholar
  44. 44.
    Wang XX, Hayakawa S, Tsuru K, Osaka A (2002) Bioactive titania gel layers formed by chemical treatment of Ti substrate with a H2O2/HCl solution. Biomaterials 23:1353–1357.  https://doi.org/10.1016/S0142-9612(01)00254-X CrossRefPubMedGoogle Scholar
  45. 45.
    Zaharia C, Tudora MR, Stancu IC, Galateanu B, Lungu A, Cincu C (2012) Characterization and deposition behavior of silk hydrogels soaked in simulated body fluid. Mater Sci Eng, C 32:945–952.  https://doi.org/10.1016/j.msec.2012.02.018 CrossRefGoogle Scholar
  46. 46.
    Shanmugam S, Buvaneswari G (2014) Copper substituted hydroxyapatite and fluorapatite: synthesis, characterization and antimicrobial properties. Ceram Int 40:15655–15662.  https://doi.org/10.1016/j.ceramint.2014.07.086 CrossRefGoogle Scholar
  47. 47.
    Paşcu EI, Stokes J, McGuinness GB (2013) Electrospun composites of PHBV, silk fibroin and nano-hydroxyapatite for bone tissue engineering. Mater Sci Eng, C 33:4905–4916.  https://doi.org/10.1016/j.msec.2013.08.012 CrossRefGoogle Scholar
  48. 48.
    Diba M, Kharaziha M, Fathi MH, Gholipourmalekabadi M, Samadikuchaksaraei A (2012) Preparation and characterization of polycaprolactone/forsterite nanocomposite porous scaffolds designed for bone tissue regeneration. Compos Sci Technol 72:716–723.  https://doi.org/10.1016/j.compscitech.2012.01.023 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ChemistryVIT UniversityVelloreIndia

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