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Polymer Bulletin

, Volume 76, Issue 1, pp 87–102 | Cite as

A facile fabrication of core–shell sodium alginate/gelatin beads for drug delivery systems

  • Ting Guo
  • Ning Zhang
  • Jinbao Huang
  • Ying Pei
  • Fang Wang
  • Keyong TangEmail author
Original Paper
  • 181 Downloads

Abstract

To improve the durability and bioavailability of alginate, a one-step extrusion method was successfully applied to prepare alginate/gelatin core–shell beads. Gelatin cross-linked with glutaraldehyde was used as the core, while sodium alginate was used as the shell. To evaluate the effect of the sodium alginate shell on the in vitro drug release properties of the beads for biomedical applications, two drug models were used: water-soluble metformin hydrochloride and water-insoluble indomethacin. The structure and properties of different core–shell beads were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and swelling tests. The results showed that the beads consist of obvious inner core and outer skin layer which coats the surface stably. In addition, the core–shell structure improved the thermal stability of the beads and the entrapment efficiency reached 90% with a sodium alginate shell. As demonstrated, there is a gradual decrease in the swelling degree as the GTA or alginate concentration increases. For indomethacin, the cumulative release was 8.7% after 360 min in HCl buffer. The anomalous transport mechanism was the predominant factor affecting the release behavior of the metformin hydrochloride-loaded beads and indomethacin loaded beads were controlled by case-II transport. This work suggests that the core–shell structure could improve the swelling properties and drug release behavior of the beads.

Keywords

Alginate Gelatin Core–shell Drug delivery system Biomedical applications 

Notes

Acknowledgements

The financial support from the National Natural Science Foundation Commission of China (Nos. 51373158, 51673177) and the Sci-Tech. Innovation Talent Foundation of Henan Province (No. 144200510018) is gratefully acknowledged.

References

  1. 1.
    Poojari R, Srivastava R (2013) Composite alginate microspheres as the next-generation egg-box carriers for biomacromolecules delivery. Expert Opin Drug Deliv 10:1061–1076CrossRefGoogle Scholar
  2. 2.
    Chen Y, Chen H et al (2010) Core shell structured hollow mesoporous nanocapsules: a potential platform for simultaneous cell imaging and anticancer drug delivery. ACS Nano 4:6001–6013CrossRefGoogle Scholar
  3. 3.
    Sun J, Tan H (2013) Alginate-based biomaterials for regenerative medicine applications. Materials 6:1285–1309CrossRefGoogle Scholar
  4. 4.
    Sagiri SS, Singh VK et al (2015) Core–shell-type organogel–alginate hybrid microparticles: a controlled delivery vehicle. Chem Eng J 264:134–145CrossRefGoogle Scholar
  5. 5.
    Matricardi P, Di Meo C et al (2013) Interpenetrating polymer networks polysaccharide hydrogels for drug delivery and tissue engineering. Adv Drug Deliv Rev 65:1172–1187CrossRefGoogle Scholar
  6. 6.
    Islan GA, Castro GR (2014) Tailoring of alginate–gelatin microspheres properties for oral Ciprofloxacin-controlled release against Pseudomonas aeruginosa. Drug Deliv 21:615–626CrossRefGoogle Scholar
  7. 7.
    Lee KY, Mooney DJ (2012) Alginate: properties and biomedical applications. Prog Polym Sci 37:106–126CrossRefGoogle Scholar
  8. 8.
    Yao R, Zhang R et al (2012) Alginate and alginate/gelatin microspheres for human adipose-derived stem cell encapsulation and differentiation. Biofabrication 4:025007CrossRefGoogle Scholar
  9. 9.
    Sakai S, Yamaguchi S et al (2008) Oxidized alginate-cross-linked alginate/gelatin hydrogel fibers for fabricating tubular constructs with layered smooth muscle cells and endothelial cells in collagen gels. Biomacromol 9:2036–2041CrossRefGoogle Scholar
  10. 10.
    Thu HE, Ng SF (2013) Gelatine enhances drug dispersion in alginate bilayer film via the formation of crystalline microaggregates. Int J Pharm 454:99–106CrossRefGoogle Scholar
  11. 11.
    Sarbon NM, Badii F et al (2013) Preparation and characterisation of chicken skin gelatin as an alternative to mammalian gelatin. Food Hydrocoll 30:143–151CrossRefGoogle Scholar
  12. 12.
    Michon C, Cuvelier G et al (1993) Concentration dependence of the critical viscoelastic properties of gelatin at the gel point. Rheol Acta 32:94–103CrossRefGoogle Scholar
  13. 13.
    Hiwale P, Lampis S et al (2011) In vitro release of lysozyme from gelatin microspheres: effect of cross-linking agents and thermoreversible gel as suspending medium. Biomacromol 12:3186–3193CrossRefGoogle Scholar
  14. 14.
    Mariod AA, Adam HF (2013) Review: gelatin, source, extraction and industrial applications. Acta Sci Pol Technol Aliment 12:135–147Google Scholar
  15. 15.
    Paques JP, van der Linden E et al (2014) Preparation methods of alginate nanoparticles. Adv Colloid Interface Sci 209:163–171CrossRefGoogle Scholar
  16. 16.
    Wu C, Fan W et al (2011) In situ preparation and protein delivery of silicate–alginate composite microspheres with core–shell structure. J R Soc Interface 8:1804–1814CrossRefGoogle Scholar
  17. 17.
    Lim MPA, Lee WL et al (2013) One-step fabrication of core–shell structured alginate-PLGA/PLLA microparticles as a novel drug delivery system for water soluble drugs. Biomater Sci 1:486–493CrossRefGoogle Scholar
  18. 18.
    Perez RA, Kim HW (2015) Core–shell designed scaffolds for drug delivery and tissue engineering. Acta Biomater 21:2–19CrossRefGoogle Scholar
  19. 19.
    Poudel BK, Pradhan R et al (2014) Preparation and characterization of alginate gel core-lipid nanocapsules for co-delivery of hydrophilic and hydrophobic anti-cancer drugs. J Pharm Investig 44:485–491CrossRefGoogle Scholar
  20. 20.
    Haidar ZS, Azari F et al (2009) Modulated release of OP-1 and enhanced preosteoblast differentiation using a core–shell nanoparticulate system. J Biomed Mater Res A 91:919–928CrossRefGoogle Scholar
  21. 21.
    Del Gaudio P, Auriemma G et al (2014) Novel co-axial prilling technique for the development of core–shell particles as delayed drug delivery systems. Eur J Pharm Biopharm 87:541–547CrossRefGoogle Scholar
  22. 22.
    Yang CH, Wang CY et al (2014) Core–shell structure microcapsules with dual pH-responsive drug release function. Electrophoresis 35:2673–2680CrossRefGoogle Scholar
  23. 23.
    Huang KS, Yang CH et al (2014) Synthesis of uniform core–shell gelatin-alginate microparticles as intestine-released oral delivery drug carrier. Electrophoresis 35:330–336CrossRefGoogle Scholar
  24. 24.
    Zheng X, Smit M et al (2005) Fragmentation behavior of silica-supported metallocene/MAO catalyst in the early stages of olefin polymerization. Macromolecules 38:4673–4678CrossRefGoogle Scholar
  25. 25.
    Mi FL, Sung HW et al (2002) Drug release from chitosan–alginate complex beads reinforced by a naturally occurring cross-linking agent. Carbohydr Polym 48:61–72CrossRefGoogle Scholar
  26. 26.
    Saarai A, Kasparkova V et al (2013) On the development and characterisation of crosslinked sodium alginate/gelatine hydrogels. J Mech Behav Biomed 18:152–166CrossRefGoogle Scholar
  27. 27.
    Kumbar SG, Soppimath KS et al (2003) Synthesis and characterization of polyacrylamide-grafted chitosan hydrogel microspheres for the controlled release of indomethacin. J Appl Polym Sci 87:1525–1536CrossRefGoogle Scholar
  28. 28.
    Işiklan N (2006) Controlled release of insecticide carbaryl from sodium alginate, sodium alginate/gelatin, and sodium alginate/sodium carboxymethyl cellulose blend beads crosslinked with glutaraldehyde. J Appl Polym Sci 99:1310–1319CrossRefGoogle Scholar
  29. 29.
    Kumar P, Singh I (2010) Formulation and characterization of tramadol-loaded IPN microgels of alginate and gelatin: optimization using response surface methodology. Acta Pharm 60:295–310CrossRefGoogle Scholar
  30. 30.
    Dong Z, Wang Q et al (2006) Alginate/gelatin blend films and their properties for drug controlled release. J Membr Sci 280:37–44CrossRefGoogle Scholar
  31. 31.
    Devi N, Kakati DK (2013) Smart porous microparticles based on gelatin/sodium alginate polyelectrolyte complex. J Food Eng 117:193–204CrossRefGoogle Scholar
  32. 32.
    Saito T, Kuramaeet R et al (2013) An ultrastrong nanofibrillar biomaterial: the strength of single cellulose nanofibrils revealed via sonication-induced fragmentation. Biomacromol 14:248–253CrossRefGoogle Scholar
  33. 33.
    Li L, Li J et al (2015) Effect of formulation variables on in vitro release of a water-soluble drug from chitosan–sodium alginate matrix tablets. Asian J Pharm Sci 10:314–321CrossRefGoogle Scholar
  34. 34.
    Peppas N (1985) Analysis of Fickian and non-Fickian drug release from polymers. Pharm Acta Helv 60:110–111Google Scholar
  35. 35.
    Krstić J, Spasojević J et al (2014) In vitro silver ion release kinetics from nanosilver/poly(vinyl alcohol) hydrogels synthesized by gamma irradiation. J Appl Polym Sci 131:40321Google Scholar
  36. 36.
    Lin N, Huang J et al (2011) Effect of polysaccharide nanocrystals on structure, properties, and drug release kinetics of alginate-based microspheres. Colloids Surf B 85:270–279CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ting Guo
    • 1
  • Ning Zhang
    • 1
  • Jinbao Huang
    • 1
  • Ying Pei
    • 1
  • Fang Wang
    • 1
  • Keyong Tang
    • 1
    Email author
  1. 1.College of Materials Science and EngineeringZhengzhou UniversityZhengzhouPeople’s Republic of China

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