Preparation of origanum minutiflorum oil-loaded core–shell structured chitosan nanofibers with tunable properties
- 221 Downloads
Novel core–shell nanofiber structures loaded by an essential oil using chitosan (CH) as a polymer have been successfully produced via the simple and effective production method of coaxial electrospinning. For this purpose, origanum minutiflorum (OM) oil was incorporated into the nanofibers. A blended form of the nanofibers (B–OM) was obtained by simply mixing OM with CH polymer solution, then this blended form was loaded separately into the core (C–OM) and the shell (S–OM) layers to obtain different composite core–shell nanofiber structures. The structures of the core and shell layers were investigated by TEM analysis. Furthermore, water contact angle analysis confirmed composition of the shell layer of each nanofiber type of B–OM, S–OM, C–OM, and differentiated it from the monolithic nanofiber of CH. The SEM images displayed the average diameter of the C–OM as 291 ± 10, while S–OM nanofibers demonstrated 284 ± 12 nm. The S–OM composite nanofibers showed the highest antibacterial activity during 24 h of the testing time. The nanofiber mats of B–OM and S–OM showed initial burst release with different profiles over an extended 7-day period of time after investigation with an in vitro drug release test. Moreover, C–OM nanofibers demonstrated prolonged time for in vitro drug release behavior with the initial burst profile at 8 h, then the release profile was relatively slow and sustained for about 7 days. The OM oil included nanofiber mats with different core–shell and blended morphologies that can hold a great promise for wound healing, antibacterial, and biomedical applications due to the controlled and tunable drug release and antibacterial activities. Another important advantage of our method over the traditional techniques is being eco-friendly, since it uses natural compound and natural polymer with controllable gas permeability of the nanofiber porous structure.
KeywordsChitosan Origanum minutiflorum oil Core–shell Nanofiber Drug delivery
The authors would like to thank Scientific Research Projects Funds (BAP 2014–614) of Eskisehir Osmangazi University for the support of this study.
- 4.Fang J, Niu H, Lin T, Wang X (2008) Applications of electrospun nanofibers. Chin Sci Bull 53(15):2265Google Scholar
- 12.Sp Z, Sinha-Ray S, Sinha-Ray S, Kristl J, Yarin AL (2015) Long-term sustained ciprofloxacin release from pmma and hydrophilic polymer blended nanofibers. Mol Pharm 13(1):295–305Google Scholar
- 13.Siegel RA, Rathbone MJ (2012) Overview of controlled release mechanisms. In: Fundamentals and applications of controlled release drug delivery, Springer, New York, p 19–43Google Scholar
- 15.Zahedi P, Rezaeian I, Ranaei-Siadat SO, Jafari SH, Supaphol P (2010) A review on wound dressings with an emphasis on electrospun nanofibrous polymeric bandages. Polym Adv Technol 21(2):77–95Google Scholar
- 22.Nurbas M, Ghorbanpoor H, Avci H (2017) An eco-friendly approach to synthesis and characterization of magnetite (Fe3O4) nanoparticles using Platanus orientalis L. leaf extrac. Dig J Nanomater Biostruct 12(4):993–1000Google Scholar
- 23.D’Souza S (2014) A review of in vitro drug release test methods for nano-sized dosage forms. Adv Pharm 2014:1–12Google Scholar
- 30.Pesavento G, Calonico C, Bilia A, Barnabei M, Calesini F, Addona R, Mencarelli L, Carmagnini L, Di Martino M, Nostro AL (2015) Antibacterial activity of Oregano, Rosmarinus and Thymus essential oils against Staphylococcus aureus and Listeria monocytogenes in beef meatballs. Food Control 54:188–199CrossRefGoogle Scholar
- 34.Ryan MP, Rea MC, Hill C, Ross RP (1996) An application in cheddar cheese manufacture for a strain of Lactococcus lactis producing a novel broad-spectrum bacteriocin, lacticin 3147. Appl Environ Microbiol 62(2):612–619Google Scholar
- 37.Wang C, Duan L, Qin J, Wu Z, Guo S (2016) Studies on antibacterial activities against S. aureus of chitosan metal chelates prepared in magnetic field. J Appl Biomater Funct Mater 14(1):80–82Google Scholar
- 39.Escárcega-Galaz AA, López-Cervantes J, Sánchez-Machado DI, Brito-Zurita OR, Campas-Baypoli ON (2017) Antimicrobial activity of chitosan membranes against Staphylococcus aureus of clinical origin. In: Enany S (ed) The rise of virulence and antibiotic resistance in Staphylococcus aureus. InTech, London, pp 109–124Google Scholar