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Polymer Bulletin

, Volume 75, Issue 9, pp 4129–4144 | Cite as

Preparation of origanum minutiflorum oil-loaded core–shell structured chitosan nanofibers with tunable properties

  • Huseyin Avci
  • Hamed Ghorbanpoor
  • Macid Nurbas
Original Paper
  • 285 Downloads

Abstract

Novel core–shell nanofiber structures loaded by an essential oil using chitosan (CH) as a polymer have been successfully produced via the simple and effective production method of coaxial electrospinning. For this purpose, origanum minutiflorum (OM) oil was incorporated into the nanofibers. A blended form of the nanofibers (B–OM) was obtained by simply mixing OM with CH polymer solution, then this blended form was loaded separately into the core (C–OM) and the shell (S–OM) layers to obtain different composite core–shell nanofiber structures. The structures of the core and shell layers were investigated by TEM analysis. Furthermore, water contact angle analysis confirmed composition of the shell layer of each nanofiber type of B–OM, S–OM, C–OM, and differentiated it from the monolithic nanofiber of CH. The SEM images displayed the average diameter of the C–OM as 291 ± 10, while S–OM nanofibers demonstrated 284 ± 12 nm. The S–OM composite nanofibers showed the highest antibacterial activity during 24 h of the testing time. The nanofiber mats of B–OM and S–OM showed initial burst release with different profiles over an extended 7-day period of time after investigation with an in vitro drug release test. Moreover, C–OM nanofibers demonstrated prolonged time for in vitro drug release behavior with the initial burst profile at 8 h, then the release profile was relatively slow and sustained for about 7 days. The OM oil included nanofiber mats with different core–shell and blended morphologies that can hold a great promise for wound healing, antibacterial, and biomedical applications due to the controlled and tunable drug release and antibacterial activities. Another important advantage of our method over the traditional techniques is being eco-friendly, since it uses natural compound and natural polymer with controllable gas permeability of the nanofiber porous structure.

Keywords

Chitosan Origanum minutiflorum oil Core–shell Nanofiber Drug delivery 

Notes

Acknowledgements

The authors would like to thank Scientific Research Projects Funds (BAP 2014–614) of Eskisehir Osmangazi University for the support of this study.

References

  1. 1.
    Huang Z-M, Zhang Y-Z, Kotaki M, Ramakrishna S (2003) A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol 63(15):2223–2253CrossRefGoogle Scholar
  2. 2.
    Sun Z, Zussman E, Yarin AL, Wendorff JH, Greiner A (2003) Compound core–shell polymer nanofibers by co-electrospinning. Adv Mater 15(22):1929–1932CrossRefGoogle Scholar
  3. 3.
    Calisir MD, Erol M, Kilic A, Avci H (2016) Photophysical properties of phosphorescent elastomeric composite nanofibers. Dyes Pigm 125:95–99CrossRefGoogle Scholar
  4. 4.
    Fang J, Niu H, Lin T, Wang X (2008) Applications of electrospun nanofibers. Chin Sci Bull 53(15):2265Google Scholar
  5. 5.
    Bhardwaj N, Kundu SC (2010) Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv 28(3):325–347CrossRefPubMedGoogle Scholar
  6. 6.
    Pakravan M, Heuzey M-C, Ajji A (2012) Core–shell structured PEO-chitosan nanofibers by coaxial electrospinning. Biomacromol 13(2):412–421CrossRefGoogle Scholar
  7. 7.
    Cheng L, Ma S, Wang T, Li X, Luo J, Li W, Mao Y, Gz D (2014) Synthesis and characterization of SnO2 hollow nanofibers by electrospinning for ethanol sensing properties. Mater Lett 131:23–26CrossRefGoogle Scholar
  8. 8.
    Li Y, Lim CT, Kotaki M (2015) Study on structural and mechanical properties of porous PLA nanofibers electrospun by channel-based electrospinning system. Polymer 56:572–580CrossRefGoogle Scholar
  9. 9.
    Hu X, Liu S, Zhou G, Huang Y, Xie Z, Jing X (2014) Electrospinning of polymeric nanofibers for drug delivery applications. J Control Release 185:12–21CrossRefPubMedGoogle Scholar
  10. 10.
    Yarin A (2011) Coaxial electrospinning and emulsion electrospinning of core–shell fibers. Polym Adv Technol 22(3):310–317CrossRefGoogle Scholar
  11. 11.
    Nguyen TTT, Chung OH, Park JS (2011) Coaxial electrospun poly (lactic acid)/chitosan (core/shell) composite nanofibers and their antibacterial activity. Carbohydr Polym 86(4):1799–1806CrossRefGoogle Scholar
  12. 12.
    Sp Z, Sinha-Ray S, Sinha-Ray S, Kristl J, Yarin AL (2015) Long-term sustained ciprofloxacin release from pmma and hydrophilic polymer blended nanofibers. Mol Pharm 13(1):295–305Google Scholar
  13. 13.
    Siegel RA, Rathbone MJ (2012) Overview of controlled release mechanisms. In: Fundamentals and applications of controlled release drug delivery, Springer, New York, p 19–43Google Scholar
  14. 14.
    Boateng JS, Matthews KH, Stevens HN, Eccleston GM (2008) Wound healing dressings and drug delivery systems: a review. J Pharm Sci 97(8):2892–2923CrossRefPubMedGoogle Scholar
  15. 15.
    Zahedi P, Rezaeian I, Ranaei-Siadat SO, Jafari SH, Supaphol P (2010) A review on wound dressings with an emphasis on electrospun nanofibrous polymeric bandages. Polym Adv Technol 21(2):77–95Google Scholar
  16. 16.
    Avci H, Guzel FD, Erol S, Akpek A (2017) Recent advances in organ-on-a-chip technologies and future challenges: a review. Turk J Chem.  http://doi.org/10.3906/kim-1611-35 CrossRefGoogle Scholar
  17. 17.
    Avci H, Monticello R, Kotek R (2013) Preparation of antibacterial PVA and PEO nanofibers containing Lawsonia Inermis (henna) leaf extracts. J Biomater Sci Polym Ed 24(16):1815–1830CrossRefPubMedGoogle Scholar
  18. 18.
    Rodriguez-Garcia I, Silva-Espinoza B, Ortega-Ramirez L, Leyva J, Siddiqui M, Cruz-Valenzuela M, Gonzalez-Aguilar G, Ayala-Zavala J (2016) Oregano essential oil as an antimicrobial and antioxidant additive in food products. Crit Rev Food Sci Nutr 56(10):1717–1727CrossRefPubMedGoogle Scholar
  19. 19.
    Aslim B, Yucel N (2008) In vitro antimicrobial activity of essential oil from endemic origanum minutiflorum on ciprofloxacin-resistant Campylobacter spp. Food Chem 107(2):602–606CrossRefGoogle Scholar
  20. 20.
    Ifuku S (2014) Chitin and chitosan nanofibers: preparation and chemical modifications. Molecules 19(11):18367–18380CrossRefPubMedGoogle Scholar
  21. 21.
    Jayakumar R, Prabaharan M, Kumar PS, Nair S, Tamura H (2011) Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol Adv 29(3):322–337CrossRefPubMedGoogle Scholar
  22. 22.
    Nurbas M, Ghorbanpoor H, Avci H (2017) An eco-friendly approach to synthesis and characterization of magnetite (Fe3O4) nanoparticles using Platanus orientalis L. leaf extrac. Dig J Nanomater Biostruct 12(4):993–1000Google Scholar
  23. 23.
    D’Souza S (2014) A review of in vitro drug release test methods for nano-sized dosage forms. Adv Pharm 2014:1–12Google Scholar
  24. 24.
    Che H, Huo M, Peng L, Ye Q, Guo J, Wang K, Wei Y, Yuan J (2015) CO2-switchable drug release from magneto-polymeric nanohybrids. Polym Chem 6(12):2319–2326CrossRefGoogle Scholar
  25. 25.
    Karthikeyan K, Guhathakarta S, Rajaram R, Korrapati PS (2012) Electrospun zein/eudragit nanofibers based dual drug delivery system for the simultaneous delivery of aceclofenac and pantoprazole. Int J Pharm 438(1):117–122CrossRefPubMedGoogle Scholar
  26. 26.
    Zhang H, Wu C, Zhang Y, White CJB, Xue Y, Nie H, Zhu L (2010) Elaboration, characterization and study of a novel affinity membrane made from electrospun hybrid chitosan/nylon-6 nanofibers for papain purification. J Mater Sci 45(9):2296–2304CrossRefGoogle Scholar
  27. 27.
    Rieger KA, Birch NP, Schiffman JD (2016) Electrospinning chitosan/poly (ethylene oxide) solutions with essential oils: correlating solution rheology to nanofiber formation. Carbohydr Polym 139:131–138CrossRefPubMedGoogle Scholar
  28. 28.
    Ballester-Costa C, Sendra E, Fernández-López J, Pérez-Álvarez JA, Viuda-Martos M (2013) Chemical composition and in vitro antibacterial properties of essential oils of four Thymus species from organic growth. Ind Crops Prod 50:304–311CrossRefGoogle Scholar
  29. 29.
    Arumugam G, Swamy MK, Sinniah UR (2016) Plectranthus amboinicus (Lour.) Spreng: botanical, phytochemical, pharmacological and nutritional significance. Molecules 21(4):369CrossRefPubMedGoogle Scholar
  30. 30.
    Pesavento G, Calonico C, Bilia A, Barnabei M, Calesini F, Addona R, Mencarelli L, Carmagnini L, Di Martino M, Nostro AL (2015) Antibacterial activity of Oregano, Rosmarinus and Thymus essential oils against Staphylococcus aureus and Listeria monocytogenes in beef meatballs. Food Control 54:188–199CrossRefGoogle Scholar
  31. 31.
    Schulz H, Özkan G, Baranska M, Krüger H, Özcan M (2005) Characterisation of essential oil plants from Turkey by IR and Raman spectroscopy. Vib Spectrosc 39(2):249–256CrossRefGoogle Scholar
  32. 32.
    Wu Y, Luo Y, Wang Q (2012) Antioxidant and antimicrobial properties of essential oils encapsulated in zein nanoparticles prepared by liquid–liquid dispersion method. LWT Food Sci Technol 48(2):283–290CrossRefGoogle Scholar
  33. 33.
    Rodríguez-Solana R, Daferera DJ, Mitsi C, Trigas P, Polissiou M, Tarantilis PA (2014) Comparative chemotype determination of Lamiaceae plants by means of GC–MS, FT-IR, and dispersive-Raman spectroscopic techniques and GC-FID quantification. Ind Crops Prod 62:22–33CrossRefGoogle Scholar
  34. 34.
    Ryan MP, Rea MC, Hill C, Ross RP (1996) An application in cheddar cheese manufacture for a strain of Lactococcus lactis producing a novel broad-spectrum bacteriocin, lacticin 3147. Appl Environ Microbiol 62(2):612–619PubMedPubMedCentralGoogle Scholar
  35. 35.
    Goy RC, Britto D, Assis OB (2009) A review of the antimicrobial activity of chitosan. Polímeros 19(3):241–247CrossRefGoogle Scholar
  36. 36.
    Arkoun M, Daigle F, Heuzey M-C, Ajji A (2017) Mechanism of action of electrospun chitosan- based nanofibers against meat spoilage and pathogenic bacteria. Molecules 22(4):585CrossRefGoogle Scholar
  37. 37.
    Wang C, Duan L, Qin J, Wu Z, Guo S (2016) Studies on antibacterial activities against S. aureus of chitosan metal chelates prepared in magnetic field. J Appl Biomater Funct Mater 14(1):80–82Google Scholar
  38. 38.
    Rodríguez-Núñez JR, López-Cervantes J, Sánchez-Machado DI, Ramírez-Wong B, Torres-Chavez P, Cortez-Rocha MO (2012) Antimicrobial activity of chitosan-based films against Salmonella typhimurium and Staphylococcus aureus. Int J Food Sci Technol 47(10):2127–2133CrossRefGoogle Scholar
  39. 39.
    Escárcega-Galaz AA, López-Cervantes J, Sánchez-Machado DI, Brito-Zurita OR, Campas-Baypoli ON (2017) Antimicrobial activity of chitosan membranes against Staphylococcus aureus of clinical origin. In: Enany S (ed) The rise of virulence and antibiotic resistance in Staphylococcus aureus. InTech, London, pp 109–124Google Scholar
  40. 40.
    Yan S, Xiaoqiang L, Lianjiang T, Chen H, Xiumei M (2009) Poly (l-lactide-co-ɛ-caprolactone) electrospun nanofibers for encapsulating and sustained releasing proteins. Polymer 50(17):4212–4219CrossRefGoogle Scholar
  41. 41.
    Mickova A, Buzgo M, Benada O, Rampichova M, Fisar Z, Filova E, Tesarova M, Lukas D, Amler E (2012) Core/shell nanofibers with embedded liposomes as a drug delivery system. Biomacromol 13(4):952–962CrossRefGoogle Scholar
  42. 42.
    Wang C, Yan K-W, Lin Y-D, Hsieh PC (2010) Biodegradable core/shell fibers by coaxial electrospinning: processing, fiber characterization, and its application in sustained drug release. Macromolecules 43(15):6389–6397CrossRefGoogle Scholar
  43. 43.
    Ramakrishna S (2005) An introduction to electrospinning and nanofibers. World Scientific, SingaporeCrossRefGoogle Scholar
  44. 44.
    Wang X, Yue T, Lee T-C (2015) Development of pleurocidin-poly (vinyl alcohol) electrospun antimicrobial nanofibers to retain antimicrobial activity in food system application. Food Control 54:150–157CrossRefGoogle Scholar
  45. 45.
    Yu H, Jia Y, Yao C, Lu Y (2014) PCL/PEG core/sheath fibers with controlled drug release rate fabricated on the basis of a novel combined technique. Int J Pharm 469(1):17–22CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Metallurgical and Materials Engineering DepartmentEskisehir Osmangazi UniversityEskisehirTurkey
  2. 2.Department of Polymer Science and TechnologyEskisehir Osmangazi UniversityEskisehirTurkey
  3. 3.Chemical Engineering DepartmentEskisehir Osmangazi UniversityEskisehirTurkey

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