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Polymer Bulletin

, Volume 75, Issue 9, pp 4103–4115 | Cite as

Microneedle-assisted transdermal delivery of acetylsalicylic acid (aspirin) from biopolymer films extracted from fish scales

  • O. Olatunji
  • M. Olubowale
  • C. Okereke
Original Paper

Abstract

This study investigates the transdermal release of aspirin (ASA) from films of hydrolyzed collagen obtained from fish scales. The patches were made with 0.2 g of ASA dissolved in 99% ethanol and 1.98 g of the extracted fish scale biopolymer (FSBP) dissolved in 10 mL distilled water which is then dried. Porcine skin is pre-treated with solid metal microneedles. ASA-loaded FSBP patch is then applied. The skin samples were placed on diffusion cells. Samples were taken at varying times: 5, 24, and 48 h and tested for the presence of ASA using UV spectrometry-based method introduced in this study. The results obtained showed that for all repeated study, no ASA was detected in the receiver compartment after 5 h. After 24 h up to 0.24 mg/mL ASA had released into the receiver compartment; and after 48 h, a concentration 0.74 mg/mL had been reached for skin samples treated with microneedles. ASA was not detected in the receiver compartment for skin samples that were not pre-treated with microneedles after 5 h. After 24 h, the concentration of 0.04 mg/mL was recorded, and at 48 h, concentration of 0.045 mg/mL was detected. This study presents for the first time transdermal drug delivery (TDD) films made of FSBP applied as drug delivery films for ASA and the use of microneedles to significantly enhance the release of ASA from the ASA-FSBP TDD films using the ‘poke and patch’ method. We also present a novel method for testing ASA in the TDD study using reaction with ferrous gluconate.

Keywords

Microneedles ASA Drug delivery Transdermal Biopolymers Acetyl salicylic acid 

Abbreviations

ASA

Acetyl salicylic acid

TDD

Transdermal drug delivery

FSBP

Fish scale biopolymer

HPLC

High-pressure liquid chromatography

LC–MS

Liquid chromatography–mass spectrometry

GC–MS

Gas chromatography–mass spectrometry

FTIR

Fourier transform infrared spectrometry

FeGluco

Ferrous gluconate

Notes

Acknowledgements

Funding was provided by the International Foundation for Science (Grant no. F/5544-1).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Chemical EngineeringUniversity of LagosLagosNigeria

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