Aureimonas leprariae sp. nov., Isolated from a Lepraria sp. Lichen
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A Gram-negative, motile, aerobic and coccoid rod-shaped bacterium, designated strain YIM132180T, was isolated from a Lepraria sp. lichen collected from Pu’er, Yunnan Province, China. The strain grew at 15–35 °C (optimum, 25–28 °C), at 0–2% (w/v) NaCl (optimum, 0–1%) and at pH 6.0–9.0 (optimum, pH 7.0). The 16S rRNA gene sequence showed that strain YIM132180T had highest similarity (96.4%) with Aureimonas endophytica 2T4P-2-4T, followed by Aureimonas ureilytica NBRC 106430T (95.7%) and Aureimonas rubiginis CC-CFT034T (95.6%). Phylogenetic analysis showed that the strain grouped with species of the genus Aureimonas. The genomic sequence was 4,779,519 bp and contained 4584 coding sequences (CDSs), 54 RNA genes, 3 complete rRNA genes and 47 tRNA genes. The major fatty acids (>10%) of strain YIM132180T were C18:1ω7c, C-16:0 and C19:0 cyclo ω8c. The predominant menaquinone was ubiquinone 10 (Q-10). The polar lipid profile comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipid, amino lipid, lipid and most importantly sulfoquinovosyldiacylglycerol (SQDG). Based on the draft genome sequence, the G +C content of strain YIM132180T was 68.4 mol%. The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic, and phylogenetic analyses, showed that strain YIM132180T represents a novel species of the genus Aureimonas, for which the name Aureimonas leprariae sp. nov. is proposed. The type strain is YIM 132180T (=KCTC 72462T = CGMCC 1.17389T).
This research was funded by National Natural Science Foundation of China (31460005).
KZ: performed the experiments and wrote the manuscript; L-QJ and G-DL: analysed the data; S-BS and Q-YL: performed the study; D-FA and LL: analysed the data; X-YW: collected the lichen samples; L-SW: identified the lichen samples; YJ: guided the experiments and revised the manuscript; C-LJ: designed the study.
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Conflict of interest
The authors declare that they have no conflict of interest.
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