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Edaphocola flava sp. nov., Isolated from Herbicide Bensulfuron-methyl-Contaminated Soil

  • Ting Hu
  • Yun Xiang
  • Zi-Yu Xing
  • Peng Qi
  • Xing HuangEmail author
Article

Abstract

A novel strain, HME-24T, was isolated from herbicide bensulfuron-methyl-contaminated soil in Jiangning District, Jiangsu Province, China. Strain HME-24T was a Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterium, and the colonies on R2A agar are yellow. The strain was non-sporulating, catalase- and oxidase-positive. Cells growth occurred at 15–37 °C (optimum, 30 °C), at pH 6.0–8.0 (optimum, 7.0) and with 0–1.5% (w/v) NaCl (optimum, 0.5% NaCl). Strain HME-24T showed the highest 16S rRNA gene sequence identity to Edaphocola aurantiacus H2T (99.58%), followed by Taibaiella chishuiensis AY17T (94.02%). The sole respiratory quinone was Menaquinone-7 (MK-7), the major polar lipids of strain HME-24T were two unidentified lipids, two unidentified aminolipids, an unidentified glycolipid and phosphatidylethanolamine, and the major fatty acids were iso-C15:0, iso-C15:1 G and iso-C17:0 3-OH. The genomic DNA G+C content values based on total genome sequences of strain HME-24T and H2T were 43.6 mol% and 43.8 mol%, respectively. Average nucleotide identity (ANI) for draft genomes between strain HME-24T and H2T was 94.8%, and the digital DNA–DNA hybridization (dDDH) for draft genomes between strain HME-24T and H2T was 59.2%. On the basis of phenotypic, genotypic and phylogenetic analysis, strain HME-24T represents a novel species of the genus Edaphocola, with the name Edaphocola flava sp. nov. being proposed. The type strain of Edaphocola flava is HME-24T (= KCTC 62977T = CGMCC 1.13935T).

Notes

Acknowledgements

This work was funded by the National Natural Science Fund of China (41671317) and the Jiangsu Agriculture Science and Technology Innovation Fund (CX(18)1005).

Compliance with Ethical Standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Research Involving Human Participants and/or Animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

284_2019_1824_MOESM1_ESM.doc (10.6 mb)
Electronic supplementary material 1 (DOC 10,852 kb)
284_2019_1824_MOESM2_ESM.seq (3.6 mb)
Electronic supplementary material 2 (SEQ 3669 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Life SciencesNanjing Agricultural UniversityNanjingPeople’s Republic of China

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