The Draft Genome Sequence of Methylophilus sp. D22, Capable of Growing Under High Concentration of Methanol

  • Zhongxue Dai
  • Feng Guo
  • Jiangfeng Ma
  • Weiliang Dong
  • Jie Zhou
  • Min JiangEmail author
  • Wenming ZhangEmail author
  • Fengxue XinEmail author


In this study, a wild type Methylophilus sp. strain D22 belonging to the family Methylophilus was isolated and characterized, which shows high tolerance towards methanol, as it can grow under 50 g/L of methanol. Methylophilus sp. strain D22 was isolated from the lake sludge in Nanjing Tech University, China. The assembled draft genome contains one circular chromosome with 3,004,398 bp, 49.7% of GC content, and 2107 predicted encoding proteins. Sequence-based genomic analysis demonstrates that the assimilation pathway of ribulose monophosphate (RuMP) pathway and dissimilation pathway of tetrahydromethanopterin (H4MPT) pathway are co-existing and contribute to the high methanol utilization efficiency.



This work was supported by the National Key Research and Development Program of China (Grant No. 2018YFA0901500), the Jiangsu Province Natural Science Foundation for Youths (Grant Nos. BK20170993, BK20170997), the Key Science and Technology Project of Jiangsu Province (Grant No. BE2016389), the National Natural Science Foundation of China (Grant Nos. 21706125, 21727818, 21706124, 31700092), and the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (Grant No. XTE1840).

Compliance with Ethical Standards

Conflict of interest

The authors have declared that there was no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjingPeople’s Republic of China
  2. 2.Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)Nanjing Tech UniversityNanjingPeople’s Republic of China

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