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Current Microbiology

, Volume 76, Issue 11, pp 1369–1373 | Cite as

Lautropia dentalis sp. nov., Isolated from Human Dental Plaque of a Gingivitis Lesion

  • Yun Kyong Lim
  • Soon-Nang Park
  • Won-Pyo Lee
  • Jeong Hwan Shin
  • Eojin Jo
  • Yeseul Shin
  • Jayoung Paek
  • Young-Hyo Chang
  • Hongik Kim
  • Joong-Ki KookEmail author
Article
  • 167 Downloads

Abstract

A novel Gram-stain-negative, motile, and facultative anaerobic coccus, strain ChDC F240T was isolated from human subgingival dental plaque of a gingivitis lesion. The phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) sequence showed that the strain belonged to the genus Lautropia. 16S rDNA of strain ChDC F240T had the highest similarity to that of Lautropia mirabilis ATCC 51599T (98.8%). Major cellular fatty acids of strain ChDC F240T were C16:0 (43.9%) and C16:1ω6C/C16:1ω7C (38.1%). Draft genome of the strain was 3,834,139 bp in length and the G+C content was 65.0 mol%. Average nucleotide identity and genome-to-genome distance values between strain ChDC F240T and L. mirabilis ATCC 51599 T were 81.99% and 28.50% (26.1–30.9%), respectively. These results reveal that strain ChDC F240T is a novel species within the genus Lautropia, for which the name Lautropia dentalis sp. nov. is proposed; type strain is ChDC F240T (= KCOM 2505T = JCM 33297T).

Notes

Acknowledgements

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science and ICT (2017M3A9B8065844), in part by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2018R1A2B5002239), and in part by the KRIBB Research Initiative Program funded by the Ministry of Science, ICT and Future Planning.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

284_2019_1761_MOESM1_ESM.docx (43 kb)
Supplementary material 1 (DOCX 42 kb) Supplementary Fig S1. Maximum likelihood (A) and the minimum evolution (B) phylogenetic trees based on 16S rDNA sequences of strain ChDC F240T and type strains of related species. Stability of phylogenetic trees was assessed by bootstrap analysis of 1,000 replicates with MEGA version 6.06 [15]. Bars indicate 0.01 (A) or 0.01 (B) changes per nucleotide position. GenBank accession numbers of 16S rDNA sequences of strains were given in parenthesis.
284_2019_1761_MOESM2_ESM.pptx (1.2 mb)
Supplementary material 2 (PPTX 1234 kb) Supplementary Fig S2. Flagella were stained using Leifson stain solution and methylene blue. Light microscope image was taken using a CX43 Biological Microscope. Arrows indicate the flagella. Original magnification: ×1,000. Bar = 30 μm.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yun Kyong Lim
    • 1
  • Soon-Nang Park
    • 1
  • Won-Pyo Lee
    • 2
  • Jeong Hwan Shin
    • 3
  • Eojin Jo
    • 1
  • Yeseul Shin
    • 4
  • Jayoung Paek
    • 4
  • Young-Hyo Chang
    • 4
  • Hongik Kim
    • 5
  • Joong-Ki Kook
    • 1
    Email author
  1. 1.Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of DentistryChosun UniversityGwangjuRepublic of Korea
  2. 2.Korean Collection for Oral Microbiology and Department of Periodontology, School of DentistryChosun UniversityGwangjuRepublic of Korea
  3. 3.Department of Laboratory MedicineInje University College of MedicineBusanRepublic of Korea
  4. 4.ABS Research Support CenterKRIBBDaejeonRepublic of Korea
  5. 5.Vitabio, Inc.DaejeonRepublic of Korea

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