Characterization of a Bi-directional Promoter OtrRp Involved in Oxytetracycline Biosynthesis
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Previous studies identified a MarR (multiple antibiotic resistance regulator) family transcription factor OtrR in the oxytetracycline biosynthetic gene cluster, which regulated the expression of an efflux pump OtrB. The genes otrB and otrR were divergent arranged and the inter-ORF (open reading frame) region between the two genes contained the promoter otrBp. In this study, we demonstrated that the reverse complementary sequence of otrBp contained the promoter of otrR, and its activity was also repressed by OtrR by sharing the same operator otrO within otrBp, and allosteric regulated by oxytetracycline. Our findings offered a solid base for the synthetic biological application of the bi-direction promoter in controlling two elements at the same time using only one signal molecule.
We dedicate this paper to and in memory of our beloved supervisor and colleague Prof. Keqian Yang, who devoted his life to the study about antibiotic biosynthesis and regulation. This work was supported by the National Natural Science Foundation of China (Grant No. 31570031) and the Ministry of Science and Technology of China (Grant No. 2013CB734000).
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Conflict of interest
The authors declare no conflict of interest.
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