Genome Analysis of Two Novel Lytic Vibrio maritimus Phages Isolated from the Coastal Surface Seawater of Qingdao, China

  • Yuye Han
  • Min Wang
  • Qian Liu
  • Yundan Liu
  • Qi Wang
  • Xueping Duan
  • Lu Liu
  • Yong Jiang
  • Hongbing Shao
  • Cui GuoEmail author


Two novel Vibrio phages, LP.1 and LP.2 that infected Vibrio maritimus R-40493, were isolated from surface seawater in Qingdao coastal area by the double-agar layer method. Morphological analysis by transmission electron microscope showed that the two phages displayed head–tail structures with icosahedral heads of 62.37 and 54.00 nm in diameter and long non-contractile tails of 119.00 and 105.20 nm in length, respectively, and can be grouped into the Siphoviridae family. Thermal and pH sensitivity tests exhibited that LP.1 was stable at temperature ranging from − 20 to 65 °C and at pH ranging from 5 to 12, and LP.2 showed vitality over a wider range of temperature (− 20–75 °C) and pH (3–12). Both LP.1 and LP.2 contained linear and double-stranded DNA genomes with a length of 46,791-bp and 37,128-bp, respectively. The genome of both phages can be classified into four functional groups, including DNA replication and regulation, phage packaging, phage structure, and additional function. The bioinformatic analysis demonstrated that the Vibrio phages LP.1 and LP.2 are novel phages. By conducting morphological, biochemical, and genomic analysis, our study provides useful information for further research on the interaction between Vibrio phages and their host.



The study was funded by National Key R&D Program of China (No. 2018YFC1406704); Open research fund of LMB (No. LMB20091001); the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (No. 2018SDKJ0406-6); Qingdao National Laboratory for Marine Science and Technology (No. 2016ASKJ14); The Fundamental Research Funds for the Central Universities (Nos. 201562018, 201812002, 201762017); State Oceanic Administration People’s republic of China (Nos. GASI-02-PAC-ST-MSwin, GASI-02-PAC-ST-MSaut); and Natural Science Foundation of China (Nos. 41076088, 41676178, 31500339).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yuye Han
    • 1
  • Min Wang
    • 1
    • 2
    • 3
  • Qian Liu
    • 1
  • Yundan Liu
    • 1
  • Qi Wang
    • 1
  • Xueping Duan
    • 1
  • Lu Liu
    • 1
  • Yong Jiang
    • 1
    • 2
    • 3
  • Hongbing Shao
    • 1
  • Cui Guo
    • 1
    • 2
    • 3
    Email author
  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoChina
  2. 2.Institute of Evolution and Marine BiodiversityOcean University of ChinaQingdaoChina
  3. 3.Key Lab of Polar Oceanography and Global Ocean ChangeOcean University of ChinaQingdaoChina

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