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Paenibacillus lutimineralis sp. nov., Isolated From Bentonite

  • Eui-Sang Cho
  • In-Tae Cha
  • Dong-Ho Seo
  • Young-Do Nam
  • Seong Woon Roh
  • Jae-Hwan KimEmail author
  • Myung-Ji SeoEmail author
Article
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Abstract

Strain MBLB1234T was isolated from bentonite samples collected at Guryong mining area located in Pohang, Republic of Korea and was taxonomically characterized by a polyphasic approach. This strain was a Gram-stain-negative, motile, endospore-forming, facultative anaerobic, catalase-positive, oxidase-negative, and rod-shaped bacterium. Strain MBLB1234T was able to grow at 20‒45 °C (optimum, 37 °C), pH 6.0‒10.0 (optimum, 7.0–8.0), and 0‒5.0% (w/v) NaCl (optimum, 0.5%). Genome size was 6,497,679 bp with a G + C content of 46.4 mol %. The genome was predicted to contain 5233 protein-coding genes, and 135 rRNA genes consisted of 10 5S rRNAs, 10 16S rRNAs, 10 23S rRNAs, and 105 tRNAs. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain MBLB1234T clustered with Paenibacillus motobuensis JCM 12774T and P. aceti JCM 31170T with 98.3–98.5% and 97.2–97.4% sequencing similarity, respectively. The major fatty acids of strain MBLB1234T were anteiso-C15:0 (35.7%), anteiso-C17:0 (17.8%), iso-C17:0 (14.5%), and C16:0 (11.0%). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, and one unidentified phospholipid, six unidentified aminophospholipids, and one unidentified lipid. The predominant isoprenoid quinone was menaquinone-7. DNA–DNA hybridization values between strain MBLB1234T and P. motobuensis JCM 12774T and P. aceti JCM 31170T were 34 and 38%, respectively. Average nucleotide identity value between strains MBLB1234T and P. aceti L14T was 82.3%. Based on characteristics of genomic, phenotypic, chemotaxonomic, and phylogenetic analyses, strain MBLB1234T represents a novel species of the genus P. , for which the name P. lutimineralis sp. nov. is proposed. The type strain is MBLB1234T (= JCM 32684T = KCTC 33978T).

Notes

Acknowledgements

This research was supported by the Basic Research Project (18-3214) of the Korea Institute of Geoscience and Mineral Resources (KIGAM) funded by the Ministry of Science and ICT. This research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03931582), and the World Institute of Kimchi (KE1902-2) funded by the Ministry of Science, ICT & Future Planning.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Bioengineering and Nano-BioengineeringGraduate School of Incheon National UniversityIncheonRepublic of Korea
  2. 2.Microbiology and Functionality Research GroupWorld Institute of KimchiGwangjuRepublic of Korea
  3. 3.Research Group of HealthcareKorea Food Research InstituteWanjuRepublic of Korea
  4. 4.Advanced Geo-materials R&D Department, Pohang BranchKorea Institute of Geoscience and Mineral ResourcesPohangRepublic of Korea
  5. 5.Division of BioengineeringIncheon National UniversityIncheonRepublic of Korea

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