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Different Virulence Capabilities and ompA Expressions in ST2 and ST513 of Multidrug-Resistant Acinetobacter baumannii

  • Armaghan Soltani Shirazi
  • Morvarid Shafiei
  • Hamid Solgi
  • Mohammad Mehdi Aslani
  • Omid AziziEmail author
  • Farzad BadmastiEmail author
Article

Abstract

Successful clones of Acinetobacter baumannii cause a variety of nosocomial infections through serum resistance, biofilm formation, and antimicrobial resistance as virulence capabilities. Fifty clinical isolates of multidrug-resistant (MDR) A. baumannii were analyzed for clonal relatedness, serum resistance, biofilm formation, and in vivo assays. Furthermore, some virulence genes, sequence variation of ompA, and its expression were studied. The MLST (multilocus sequence typing) results showed that there were three sequence types among MDR isolates including ST2 (64%, 32/50), ST513 (30%, 15/50), and ST1 (6%, 3/50). The data showed that the clinical isolates recovered from sputum had mostly high biofilm-formation capacity, while isolates recovered from host interior fluids had high serum resistance. The results of PCR assays and in silico analysis represented patterns of virulence genes and even ompA sequence variations among MDR isolates which were clonally dependent. While quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that bacteremia-producing strains in C57/BL6 mice significantly overexpress ompA (P < 0.05) and have a direct relation with the level of IL-6 in bloodstream of mice. Moreover, the expressions of ompA among indistinguishable clones (ST2 or ST513) were clonally independent.

Notes

Acknowledgments

The authors would like to thank the personnel in the Bacteriology Department of the Pasture Institute of Iran for their help. This research was supported by the Pasture Institute of Iran under Grant No. B-0137.

Compliance with Ethical Standards

Conflict of interest

The authors have no conflicts of interest to declare.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Armaghan Soltani Shirazi
    • 1
  • Morvarid Shafiei
    • 1
  • Hamid Solgi
    • 2
  • Mohammad Mehdi Aslani
    • 1
  • Omid Azizi
    • 3
    • 4
    Email author
  • Farzad Badmasti
    • 1
    Email author
  1. 1.Department of BacteriologyPasteur Institute of IranTehranIran
  2. 2.Department of Microbiology, Faculty of MedicineIsfahan University of Medical SciencesIsfahanIran
  3. 3.Department of Laboratory Sciences, School of Paramedical SciencesTorbat Heydariyeh University of Medical SciencesTorbat HeydariyehIran
  4. 4.Health Sciences Research CenterTorbat Heydariyeh University of Medical SciencesTorbat HeydariyehIran

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