The Genome Sequence of a Novel Cyanophage S-B64 from the Yellow Sea, China
- 61 Downloads
A novel cyanophage, S-B64, which can infect marine Synechococcus WH8102, was isolated from the coastal waters of the Yellow Sea using the liquid serial dilution method. Morphological study by transmission electron microscopy revealed that the cyanophage belongs to Podovirus. It’s genome, which was completely sequenced, contains a 151,867 bp DNA molecule with a G+C content of 41.78% and 186 potential open reading frames. The functions of the genes include cyanophage structure, cyanophage packaging, DNA replication and regulation. After primary characterization, it was found that the latent period is about 3 h, and it lysed after 8 h, the burst size is about 23 virions per cell. This information will provide an important benchmark for further research on the interaction between cyanophages and their hosts.
This study was support by the fund from the National Natural Science Foundation of China (No. 31500339, 41676178 and 41076088), and the Marine Scientific and Technological Innovation Project Financially Supported by Pilot National Laboratory for Marine Science and Technology (Qingdao) (2018SDKJ0406-6, 2016ASKJ14), at the same time, thanks are due to the Fundamental Research Funds for the Central University of Ocean University of China (Grant nos. 201812002, 201762017, 201562018).
- 1.Waterbury J et al (1986) Biological and ecological characterization of the marine unicellular cyanobacterium Synechococcus. Science 214:71–120Google Scholar
- 5.Suttle CA (2000) Cyanophages and their role in the ecology of cyanobacteria. In: Whitton BA, Potts M (eds) The ecology of cyanobacteria. Springer, Dordrecht, pp 563–589Google Scholar
- 6.Wilson WH, Joint IR, Carr NG et al (1993) Isolation and molecular characterization of five marine cyanophages propagated on Synechococcus sp. strain WH7803. Appl Environ Microbiol 59(11):3736–3743Google Scholar
- 15.Waterbury JB et al (1993) Resistance to co-occurring phages enables marine Synechococcus communities to coexist with cyanophages abundant in seawater. Appl Environ Microbiol 59(10):3393–3399Google Scholar