Synergy Over Monotherapy

  • Zuzana JežíkováEmail author
  • Tomáš Pagáč
  • Ján Víglaš
  • Barbora Pfeiferová
  • Katarína Šoltys
  • Helena Bujdáková
  • Lucia Černáková
  • Petra Olejníková


Pathogenic fungi, as an increasing global threat to human health, represent a sizable risk. However, significant attention should also be paid to the yeast biofilms. One promising strategy for combating resistant microbes, as well as fungal biofilms, is to extend the lifespan and efficacy of our currently employed drugs by using combination therapy. Since the application of combined therapy of fungal infections is currently accepted, we have decided to verify the efficacy of derivative H in combination with fluconazole on C. albicans biofilm. The main advantage of synergy over monotherapy lies in reducing or even completely preventing the induction of resistance of fungal cells. We have decided to verify the derivative H (1,4-dihydropyridine-2,3,5-tricarboxylate), an intermediate of nilvadipine synthesis, in the resistance of C. albicans to fluconazole. Therefore, we have focused on the influence of derivative H on the gene expression of the main C. albicans adhesin (ALS3), which is important for the tissue colonization during the infection process. Our results show that the newly synthesized derivative H had an impact on biofilm eradication. The effect of biofilm diminution could, therefore, be explained as derivative H preventing the adherence of C. albicans cells. This study supports even more the attractiveness of this substance as a potential agent that could be used in synergy with commonly used azoles to treat various fungal infections.



This work was supported by the Project APVV-0719-12 and VEGA Project 1/0697/18.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Biochemistry and Microbiology, Faculty of Chemical and Food TechnologySlovak University of Technology in BratislavaBratislavaSlovakia
  2. 2.Comenius University Science ParkComenius UniversityBratislavaSlovakia
  3. 3.Department of Molecular Biology, Faculty of Natural SciencesComenius UniversityBratislava 4Slovakia
  4. 4.Department of Microbiology and Virology, Faculty of Natural SciencesComenius UniversityBratislava 4Slovakia

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