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Current Microbiology

, Volume 76, Issue 3, pp 376–381 | Cite as

4-Methyl-5-Pentylbenzene-1,3-Diol Regulates Chemotactic Cell Aggregation and Spore Maturation Via Different Mechanisms in Dictyostelium discoideum

  • Anna P. Kondo
  • Takaaki B. Narita
  • Chihiro Murata
  • Tetsuhiro Ogura
  • Ayame Mikagi
  • Toyonobu Usuki
  • Tamao SaitoEmail author
Article

Abstract

4-Methyl-5-pentylbenzene-1,3-diol (MPBD), a product of the polyketide synthase SteelyA, is a signaling molecule that regulates Dictyostelium discoideum development. During early development, MPBD controls chemotactic cell aggregation by regulating the expression of genes in the cAMP signaling pathway; however, during culmination at late development, it induces spore maturation. In the present study, we analyzed the effects of MPBD, its derivatives, and a putative MPBD-derived metabolite on developmental defects in the MPBD-less stlA null mutant. Using structure–activity relationship studies, it was observed that in MPBD, the functional groups that were essential for induction of spore maturation were different from those essential for induction of cell aggregation. Dictyoquinone, a putative MPBD metabolite rescued the aggregation defect in stlA null mutant in early development, but not the spore maturation defect at the later stage. Our data suggest that MPBD regulates chemotactic cell aggregation and spore maturation via different mechanisms.

Notes

Acknowledgements

We would like to thank Mr. Shuhei Narita (Sophia University) for performing preliminary experiments. We would like to thank Dr. Kei Inouye and Dr. Akiko Oohata for their valuable comments and suggestions for this work.

Funding

This study was funded by JSPS KAKENHI [Grant Number 15K01807] to T.S. and a Sophia University Collaborative Research Grant (to T.S. and T.U.).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

284_2019_1639_MOESM1_ESM.docx (7.5 mb)
Supplementary material 1 (DOCX 7651 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Graduate School of Science and TechnologySophia UniversityTokyoJapan
  2. 2.Faculty of Science and TechnologySophia UniversityTokyoJapan

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