Current Microbiology

, Volume 76, Issue 3, pp 355–360 | Cite as

Effects of Different Carbon Sources on Enzyme Production and Ultrastructure of Cellulosimicrobium cellulans

  • Tong-Yi DouEmail author
  • Jing Chen
  • Yi-Fu Hao
  • Xiaohui Qi


The secretomes of the strain Cellulosimicrobium cellulans F16 grown on different carbon sources were analyzed by zymography, and the subcellular surface structures were extensively studied by electron microscope. The exo-cellulase and xylanase systems were sparse when cells were grown on soluble oligosaccharides, but were significantly increased when grown on complex and water-insoluble polysaccharides, such as Avicel, corn cob, and birchwood xylan. The cellulosome-like protuberant structures were clearly observed on the cell surfaces of strain F16 grown on cellulose, with diameters of 15–20 nm. Fibrous structures that connected the adjacent cells can be seen under microscope. Moreover, protuberances that adsorbed the cell to cellulose were also observed. As the adhesion of Cellulosimicrobium cellulans cells onto cellulose surfaces occurs via thick bacterial curdlan-type exopolysaccharides (EPS), such surface layer is potentially important in the digestion of insoluble substrates such as cellulose or hemicellulose, and the previously reported xylanosomes are part of such complex glycocalyx layer on the surface of the bacterial cell.











Scanning electron microscopy


Transmission electron microscopy


Microcrystalline cellulose


Cationized ferritin



This work was supported by the National Natural Science Foundation of China (No. 31600641), the Fundamental Research Funds for the Central Universities (No. DUT18RC(4)057), and the National Key Research and Development Program of China (2017YFC1702006).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Bayer EA, Lamed R, White BA, Flint HJ (2008) From cellulosomes to cellulosomics. Chem Rec 8(6):364–377CrossRefGoogle Scholar
  2. 2.
    Artzi L, Bayer EA, Morais S (2017) Cellulosomes: bacterial nanomachines for dismantling plant polysaccharides. Nat Rev Microbiol 15(2):83–95CrossRefGoogle Scholar
  3. 3.
    Lamed R, Morag E, Moryosef O, Bayer EA (1991) Cellulosome-like entities in Bacteroides cellulosolvens. Curr Microbiol 22(1):27–33CrossRefGoogle Scholar
  4. 4.
    Bayer EA, Shimon LJ, Shoham Y, Lamed R (1998) Cellulosomes-structure and ultrastructure. J Struct Biol 124(2–3):221–234CrossRefGoogle Scholar
  5. 5.
    Fontes CMGA, Gilbert HJ (2010) Cellulosomes: highly efficient nanomachines designed to designed to deconstruct plant cell wall complex carbohydrates. Annu Rev Biochem 79:655–681CrossRefGoogle Scholar
  6. 6.
    Artzi L, Dadosh T, Milrot E, Morais S, Levin-Zaidman S, Morag E, Bayer EA (2018) Colocalization and disposition of cellulosomes in Clostridium clariflavum as revealed by correlative superresolution imaging. MBio 9(1):e00012–e00018CrossRefGoogle Scholar
  7. 7.
    Lamed R, Naimark J, Morgenstern E, Bayer EA (1987) Specialized cell-surface structures. Cellulolytic Bacteria J Bacteriol 169(8):3792–3800CrossRefGoogle Scholar
  8. 8.
    Kenyon WJ, Esch SW, Buller CS (2005) The curdlan-type exopolysaccharide produced by Cellulomonas flavigena KU forms part of an extracellular glycocalyx involved in cellulose degradation. Antonie Van Leeuwenhoek 87(2):143–148CrossRefGoogle Scholar
  9. 9.
    Vladuttalor M, Kauri T, Kushner DJ (1986) Effects of cellulose on growth, enzyme-production, and ultrastructure of a Cellulomonas species. Arch Microbiol 144(3):191–195CrossRefGoogle Scholar
  10. 10.
    Dou TY, Luan HW, Liu XB, Li SY, Du XF, Yang L (2015) Enzymatic hydrolysis of 7-xylosyltaxanes by an extracellular xylosidase from Cellulosimicrobium cellulans. Biotechnol Lett 37(9):1905–1910CrossRefGoogle Scholar
  11. 11.
    Dou TY, Luan HW, Ge GB, Dong MM, ZH F, Cui QHY, Wang P, Hao JY, Yang DC, Yang SL L (2015) Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel. Sci Rep 5:13768CrossRefGoogle Scholar
  12. 12.
    Garrity GM, Goodfellow M, Whitman WB, Kämpfer P, Busse H-J, Ludwig W, Trujillo M, Suzuki KI, Parte A (2012) The Actinobacteria. In: Bergey’s manual of systematic bacteriology, vol 5. 2 edn. Springer, New York, pp 995–1016Google Scholar
  13. 13.
    Wang W, Yu Y, Dou TY, Wang JY, Sun C (2018) Species of family Promicromonosporaceae and family Cellulomonadeceae that produce cellulosome-like multiprotein complexes. Biotechnol Lett 40(2):335–341CrossRefGoogle Scholar
  14. 14.
    Ferrer P (2006) Revisiting the Cellulosimicrobium cellulans yeast-lytic β-1,3-glucanases toolbox: a review. Microb Cell Fact 5:10–10CrossRefGoogle Scholar
  15. 15.
    Andrews BA, Asenjo JA (1987) Continuous-culture studies of synthesis and regulation of extracellular beta(1–3) glucanase and protease enzymes from Oerskovia xanthineolytica. Biotechnol Bioeng 30(5):628–637CrossRefGoogle Scholar
  16. 16.
    Vandooren J, Geurts N, Martens E, Van den Steen PE, Opdenakker G (2013) Zymography methods for visualizing hydrolytic enzymes. Nat Methods 10(3):211–220CrossRefGoogle Scholar
  17. 17.
    Deutscher J (2008) The mechanisms of carbon catabolite repression in bacteria. Curr Opin Microbiol 11(2):87–93CrossRefGoogle Scholar
  18. 18.
    Oh M, Kim JH, Yoon JH, Schumann P, Kim W (2018) Cellulosimicrobium arenosum sp. nov., Isolated from marine sediment sand. Curr Microbiol 75(7):901–906CrossRefGoogle Scholar
  19. 19.
    Scheller HV, Ulvskov P (2010) Hemicelluloses. Annu Rev Plant Biol 61:263–289CrossRefGoogle Scholar
  20. 20.
    Gibson LJ (2012) The hierarchical structure and mechanics of plant materials. J R Soc Interface 9(76):2749–2766CrossRefGoogle Scholar
  21. 21.
    Danon D, Goldstein L, Marikovsky Y, Skutelsky E (1972) Use of cationized ferritin as a label of negative charges on cell surfaces. J Ultrastruct Res 38(5):500–510CrossRefGoogle Scholar
  22. 22.
    Bayer EA, Setter E, Lamed R (1985) Organization and distribution of the cellulosome in Clostridium thermocellum. J Bacteriol 163(2):552–559Google Scholar
  23. 23.
    Bayer EA, Lamed R (1986) Ultrastructure of the cell-surface cellulosome of Clostridium-thermocellum and its interaction with cellulose. J Bacteriol 167(3):828–836CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Life Science and MedicineDalian University of TechnologyPanjinChina
  2. 2.College of Life ScienceDalian Minzu UniversityDalianChina

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