Current Microbiology

, Volume 76, Issue 3, pp 320–328 | Cite as

PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.

  • Katarina D. Novovic
  • Milka J. Malesevic
  • Brankica V. Filipic
  • Nemanja L. Mirkovic
  • Marija S. Miljkovic
  • Milan O. Kojic
  • Branko U. JovčićEmail author


Pseudomonas aeruginosa, which is a clinically important representative of Pseudomonas spp., has been recognized as causative agent of severe nosocomial infections worldwide. An increase in antibiotic resistance of P. aeruginosa clinical strains could be attributed to their capacity to acquire resistance through mobile genetic elements such as mobile integrons that are present in one-half of multidrug-resistant P. aeruginosa strains. Mobile class 1 integrons are recognized as genetic elements involved in the rapid dissemination of multiple genes encoding for antibiotic resistance. The LexA protein is a major repressor of integrase transcription, but differences in transcription regulation among bacterial species have also been noted. In this study, the promoter activity of class 1 integron integrase gene (intI1) and its variant lacking the LexA binding site in Pseudomonas putida WCS358 wild type, ΔrpoS and ΔpsrA was analysed. The results show that the activity of the intI1 gene promoter decreased in the rpoS and psrA mutants in the stationary phase of growth compared to the wild type, which indicates the role of RpoS and PsrA proteins in the positive regulation of integrase transcription. Additionally, it was determined that the activity of the lexA gene promoter decreased in ΔrpoS and ΔpsrA, and thus, we propose that PsrA indirectly regulates the intI1 gene promoter activity through regulation of lexA gene expression in co-operation with some additional regulators. In this study, intI1 gene expression was shown to be controlled by two major stress response (SOS and RpoS) regulons, which indicates that integrase has evolved to use both systems to sense the cell status.



This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia [Grant No. 173019].

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Katarina D. Novovic
    • 1
  • Milka J. Malesevic
    • 1
  • Brankica V. Filipic
    • 1
    • 2
  • Nemanja L. Mirkovic
    • 1
  • Marija S. Miljkovic
    • 1
  • Milan O. Kojic
    • 1
  • Branko U. Jovčić
    • 1
    • 3
    Email author
  1. 1.Institute of Molecular Genetics and Genetic EngineeringUniversity of BelgradeBelgradeSerbia
  2. 2.Faculty of PharmacyUniversity of BelgradeBelgradeSerbia
  3. 3.Faculty of BiologyUniversity of BelgradeBelgradeSerbia

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